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The map label for this gene is ybiV [H]

Identifier: 59714330

GI number: 59714330

Start: 1289296

End: 1290093

Strand: Reverse

Name: ybiV [H]

Synonym: VF_A1147

Alternate gene names: 59714330

Gene position: 1290093-1289296 (Counterclockwise)

Preceding gene: 59714331

Following gene: 59714328

Centisome position: 96.98

GC content: 37.84

Gene sequence:

>798_bases
ATGCAAAAACATAACGTAAAATTTATTGCTGCTGATATGGACGGCACACTGCTTAATGAACATAGCGAATTAGATCCAAG
TTTTTTCGATCTTTATCAGCAATTAGAAGATAAAAACATCATTTTTGCCGCTGCCTCTGGGCGTCAATACTACAGCTTGT
TAGAAACGTTTGAGCCAGTAAAAGATCGCATGATGTTTATTGCTGAAAATGGCACGTTAGTGATGTATCCCGGTGAAGAA
CTTTATAGCTGTTCAATGGATACGCCATCGGTAATCAATATCATTAAAACTGCTCGTGCGATTGAAGGTACACACATTGT
GCTTTGTGGCAAACGTTCAGCATACATTGAAACACAAGATCCAAAAGCACTGGCTGAATTTTCAAAATATTACCAGCGTT
GTGAATATGTCGAAGACTTACTTGCGGTAGATGACGAGTTTATTAAAGTAGCGATTTGTCATTTTGATGGAACTGAAGAG
TTCGTTTTCCCATCTTTTGATGCTAACTTTGGTGAGAGTCATCAAGTGGTGGTGAGTGCTAAGATCTGGCTTGATGTAAT
GAATGTGGTTGCTTCTAAAGGAGCTGCAATCAAACATCTACAAAACACACTGGACTTTAGTTTTGAAGAAACAATGAGCT
TTGGTGATTACTTAAATGATTTAGAAATGCTGCAAGAGAGTTATTACTCTTATGCGATGGAAAATGCACATCAAAAAGTA
AAAGAGACAGCACGCTTTATGGCTCCAAGTAATAAAGATGCAGGGGTATTTACCGTGATTAAAGAGCAAGTGTTGTAA

Upstream 100 bases:

>100_bases
GTAGCCTCTCATTTTACGGTGGAGTTGTTCGGTGTAACGTCCTGAACAGTATATACTTCTGGGTGTTTATTGAATTTAAA
TTATCTCTGAGTACCAATTA

Downstream 100 bases:

>100_bases
GCGTTTACTTATCGCATACTCGATATTAAAAAGCCCTAACAACACGTTAGGGCTTTTGGTTTTCTCATCGAACCATTATG
CTGGTGTTAATTCGCCATCA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQKHNVKFIAADMDGTLLNEHSELDPSFFDLYQQLEDKNIIFAAASGRQYYSLLETFEPVKDRMMFIAENGTLVMYPGEE
LYSCSMDTPSVINIIKTARAIEGTHIVLCGKRSAYIETQDPKALAEFSKYYQRCEYVEDLLAVDDEFIKVAICHFDGTEE
FVFPSFDANFGESHQVVVSAKIWLDVMNVVASKGAAIKHLQNTLDFSFEETMSFGDYLNDLEMLQESYYSYAMENAHQKV
KETARFMAPSNKDAGVFTVIKEQVL

Sequences:

>Translated_265_residues
MQKHNVKFIAADMDGTLLNEHSELDPSFFDLYQQLEDKNIIFAAASGRQYYSLLETFEPVKDRMMFIAENGTLVMYPGEE
LYSCSMDTPSVINIIKTARAIEGTHIVLCGKRSAYIETQDPKALAEFSKYYQRCEYVEDLLAVDDEFIKVAICHFDGTEE
FVFPSFDANFGESHQVVVSAKIWLDVMNVVASKGAAIKHLQNTLDFSFEETMSFGDYLNDLEMLQESYYSYAMENAHQKV
KETARFMAPSNKDAGVFTVIKEQVL
>Mature_265_residues
MQKHNVKFIAADMDGTLLNEHSELDPSFFDLYQQLEDKNIIFAAASGRQYYSLLETFEPVKDRMMFIAENGTLVMYPGEE
LYSCSMDTPSVINIIKTARAIEGTHIVLCGKRSAYIETQDPKALAEFSKYYQRCEYVEDLLAVDDEFIKVAICHFDGTEE
FVFPSFDANFGESHQVVVSAKIWLDVMNVVASKGAAIKHLQNTLDFSFEETMSFGDYLNDLEMLQESYYSYAMENAHQKV
KETARFMAPSNKDAGVFTVIKEQVL

Specific function: Catalyzes the hydrolysis of sugar phosphate to sugar and inorganic phosphate. Has a wide substrate specificity catalyzing the hydrolysis of fructose-1-P most efficiently, but it remains uncertain if this is the real substrate in vivo [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family. SupH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787043, Length=259, Percent_Identity=32.4324324324324, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI87081790, Length=258, Percent_Identity=30.6201550387597, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI2367265, Length=274, Percent_Identity=24.8175182481752, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994981, Length=241, Percent_Identity=23.6514522821577, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: =3.1.3.23 [H]

Molecular weight: Translated: 30180; Mature: 30180

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKHNVKFIAADMDGTLLNEHSELDPSFFDLYQQLEDKNIIFAAASGRQYYSLLETFEPV
CCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
KDRMMFIAENGTLVMYPGEELYSCSMDTPSVINIIKTARAIEGTHIVLCGKRSAYIETQD
HHHEEEEEECCEEEEECCHHHEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCC
PKALAEFSKYYQRCEYVEDLLAVDDEFIKVAICHFDGTEEFVFPSFDANFGESHQVVVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCEEEEEH
KIWLDVMNVVASKGAAIKHLQNTLDFSFEETMSFGDYLNDLEMLQESYYSYAMENAHQKV
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETARFMAPSNKDAGVFTVIKEQVL
HHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQKHNVKFIAADMDGTLLNEHSELDPSFFDLYQQLEDKNIIFAAASGRQYYSLLETFEPV
CCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
KDRMMFIAENGTLVMYPGEELYSCSMDTPSVINIIKTARAIEGTHIVLCGKRSAYIETQD
HHHEEEEEECCEEEEECCHHHEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCC
PKALAEFSKYYQRCEYVEDLLAVDDEFIKVAICHFDGTEEFVFPSFDANFGESHQVVVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCEEEEEH
KIWLDVMNVVASKGAAIKHLQNTLDFSFEETMSFGDYLNDLEMLQESYYSYAMENAHQKV
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETARFMAPSNKDAGVFTVIKEQVL
HHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]