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The map label for this gene is dmlR [H]

Identifier: 59714231

GI number: 59714231

Start: 1182759

End: 1183688

Strand: Reverse

Name: dmlR [H]

Synonym: VF_A1048

Alternate gene names: 59714231

Gene position: 1183688-1182759 (Counterclockwise)

Preceding gene: 59714234

Following gene: 59714228

Centisome position: 88.98

GC content: 38.17

Gene sequence:

>930_bases
ATGGATAAGTTTTCTGATATGACGATGTTTGTGAGTATCGTAAAGCATCAAGGCTTAGCGGCAGCAGGGCGAGAGCTTGG
TTTATCACCTGCAACGATGACAGCCAGACTTCAAGCTCTTGAAGAAAGATATCGAGTGAAATTACTAAATCGCAGTACAC
GACATGTGTCTCTAACCGATGCGGGGGAGCTTTATTACAAAGCCAGTCTTGAAATTTTAGATAATGTAAACGAAACCGAG
AATTTGATTTTAAACGGAGTTAAAGAGGTTAAAGGTGCGTTAAGAGTGGCAGCGCCTAAAGACATTGGTAAGCAATATAT
TCTTCCTATCTTGTCTGAATTTTGTGAAAAATACCCAGACGTAGTGCCATATTTATATTTGAATGATGATGTTTCAAATA
TTGCCGAATCAGGCATAGATATTGTCGTTCGATATGGTGAACTGGCCGATAGCAGTTTGATATCTCGACGATTATCATCT
AGCCATCGAGTGTTGTGTGCATCACCTGACTATTTAGCTAAGTATGGGACGCCTTTAACTCCACAAGATTTGATTAATCA
TCATTGTCTTGCGATGGTTCGTAGCCATGAGGAATTAAAAACATGGCATTTCCAAGGCTCTGATAAAAAGAATGTTATTA
CTGTTTTACCTAAGCGATTTTCAGATGATGGAGAAGTTGTACGTTATTGGGCTCTTGAAGGGGCTGGGATAGCATTAAAA
TCCATATTAGATGTGCAAGATGACATTAATAATCGACGCCTTGTTACTGTTCTTAATGGATATATGGCCAATTTTAATAC
ATCGACATCTGTATCAAGTGCTGATCTTAATGTGGTATACATGAGTAAAAAATATCAGCCAAAACGTATCCGGTTATTCT
TAGATTTTCTTTTGGAGAGATTTGAGACTATGAAAAACGGTGGTAAGTGA

Upstream 100 bases:

>100_bases
TAGTATATTCATTCGATTTATTTTGATAATTGAGTAATATATAAAATCATTATTCGGAAAATCTGAATAATGTGTATTCG
TAAAATTAAAGGGGCGATAG

Downstream 100 bases:

>100_bases
GATTAGAACCCTCTCTGATTTGCTTATATCAATTCAGAGAGGGGATTTTACAGAGGTTTACTTTACATCATTACTAGGCT
TGCTGTACCTAAGAAAGCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDKFSDMTMFVSIVKHQGLAAAGRELGLSPATMTARLQALEERYRVKLLNRSTRHVSLTDAGELYYKASLEILDNVNETE
NLILNGVKEVKGALRVAAPKDIGKQYILPILSEFCEKYPDVVPYLYLNDDVSNIAESGIDIVVRYGELADSSLISRRLSS
SHRVLCASPDYLAKYGTPLTPQDLINHHCLAMVRSHEELKTWHFQGSDKKNVITVLPKRFSDDGEVVRYWALEGAGIALK
SILDVQDDINNRRLVTVLNGYMANFNTSTSVSSADLNVVYMSKKYQPKRIRLFLDFLLERFETMKNGGK

Sequences:

>Translated_309_residues
MDKFSDMTMFVSIVKHQGLAAAGRELGLSPATMTARLQALEERYRVKLLNRSTRHVSLTDAGELYYKASLEILDNVNETE
NLILNGVKEVKGALRVAAPKDIGKQYILPILSEFCEKYPDVVPYLYLNDDVSNIAESGIDIVVRYGELADSSLISRRLSS
SHRVLCASPDYLAKYGTPLTPQDLINHHCLAMVRSHEELKTWHFQGSDKKNVITVLPKRFSDDGEVVRYWALEGAGIALK
SILDVQDDINNRRLVTVLNGYMANFNTSTSVSSADLNVVYMSKKYQPKRIRLFLDFLLERFETMKNGGK
>Mature_309_residues
MDKFSDMTMFVSIVKHQGLAAAGRELGLSPATMTARLQALEERYRVKLLNRSTRHVSLTDAGELYYKASLEILDNVNETE
NLILNGVKEVKGALRVAAPKDIGKQYILPILSEFCEKYPDVVPYLYLNDDVSNIAESGIDIVVRYGELADSSLISRRLSS
SHRVLCASPDYLAKYGTPLTPQDLINHHCLAMVRSHEELKTWHFQGSDKKNVITVLPKRFSDDGEVVRYWALEGAGIALK
SILDVQDDINNRRLVTVLNGYMANFNTSTSVSSADLNVVYMSKKYQPKRIRLFLDFLLERFETMKNGGK

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=31.1036789297659, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=31.6831683168317, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=31.1643835616438, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=30.9210526315789, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789639, Length=304, Percent_Identity=28.2894736842105, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=28.7625418060201, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788706, Length=187, Percent_Identity=28.8770053475936, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=19.1275167785235, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=262, Percent_Identity=25.1908396946565, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=22.9166666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790011, Length=306, Percent_Identity=21.8954248366013, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34813; Mature: 34813

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFSDMTMFVSIVKHQGLAAAGRELGLSPATMTARLQALEERYRVKLLNRSTRHVSLTD
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
AGELYYKASLEILDNVNETENLILNGVKEVKGALRVAAPKDIGKQYILPILSEFCEKYPD
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCC
VVPYLYLNDDVSNIAESGIDIVVRYGELADSSLISRRLSSSHRVLCASPDYLAKYGTPLT
CCCEEEECCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
PQDLINHHCLAMVRSHEELKTWHFQGSDKKNVITVLPKRFSDDGEVVRYWALEGAGIALK
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHH
SILDVQDDINNRRLVTVLNGYMANFNTSTSVSSADLNVVYMSKKYQPKRIRLFLDFLLER
HHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
FETMKNGGK
HHHHHCCCC
>Mature Secondary Structure
MDKFSDMTMFVSIVKHQGLAAAGRELGLSPATMTARLQALEERYRVKLLNRSTRHVSLTD
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
AGELYYKASLEILDNVNETENLILNGVKEVKGALRVAAPKDIGKQYILPILSEFCEKYPD
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCC
VVPYLYLNDDVSNIAESGIDIVVRYGELADSSLISRRLSSSHRVLCASPDYLAKYGTPLT
CCCEEEECCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
PQDLINHHCLAMVRSHEELKTWHFQGSDKKNVITVLPKRFSDDGEVVRYWALEGAGIALK
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHH
SILDVQDDINNRRLVTVLNGYMANFNTSTSVSSADLNVVYMSKKYQPKRIRLFLDFLLER
HHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
FETMKNGGK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]