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The map label for this gene is agaD [H]

Identifier: 59714184

GI number: 59714184

Start: 1125152

End: 1126069

Strand: Reverse

Name: agaD [H]

Synonym: VF_A1001

Alternate gene names: 59714184

Gene position: 1126069-1125152 (Counterclockwise)

Preceding gene: 59714185

Following gene: 59714183

Centisome position: 84.65

GC content: 40.2

Gene sequence:

>918_bases
ATGGTATCTAAAACAGAAAATCCGAACGCTCTAATTGATGGCATTGATGAGTTTCAAGGTACTCAAGTGGCGAATCCTAA
GAGCTTGGTTGATGGTATCGACGAATATCAAGACAAGGAAGTTCGTAAAGTTATCTCGAAAAAAGATCTTTGGAAATGTG
CAATTCGTGGCCTTTTCATGGAAGGGAACTTCAACTTTGAACGTATGCAAGGTGGTGGCTTTGCTTACTCAATTGTTCCT
GCACTAAAGAAGATTCATGGTAATAACAAACGTGATTTAGCAACGGCAACTAAGAACCATCTTCAGTTCTTTAATGCGAG
TCCTAAACTGTTTACTTTCTTGCTAGGTACTGCCATTGCAATGGAAGAAAACAAGGAAAAACCGTCTACAGTAAATGTAA
TGAAAGTAGCGGGTATGGGACCTACTGGTGGTATTGGTGATGCAATTGACCATATGACTCTTATGCCGTTAACGTTGGCG
TTAGGTGCATCAATTGCAATGCAAGGCTCTATTGCAGGACCGTTTGTTTTCTTCATTTTGTATCAAATTGTGCACTTCTT
TGTGTATTTCGGTTTGATGTTTATGGGATATAAAGCCGGTACAGCAGCCATGGTTAACATGAGCGATGCGACTGAGAAAT
TAGCCAAAGCGGCAAATATCATGGGTATCTTTGTTATTGGTGCATTGTCGGCATCCTTTATTAAAGTTCAAACCACCTTA
TCTCTCGAACTCGGAGGGAAAGTCGTTGAACTTCAAACCGCACTGTTTGACAAGATAATGCCTAATATTTTGCCACTAGC
ATTGGTGTTCTTTATGTTAAAAATGGTGAAAGGAACTGGTTTTTGGGCGAAACCTCCTGTGTTGATTTTCTCAACGTTAG
CCGGTGGTGTTATTGGTCACTACCTAGGTATTTTGTAA

Upstream 100 bases:

>100_bases
ATGGCCGTGGCAATTTTAGGGGCATCAGCAGCCGCTCTATATTACTTCTTTAGTGGAAATGGAAACAAAAATTCAAATCA
AGAATTTGAGGACTTTGAAG

Downstream 100 bases:

>100_bases
CACAAAAAGAAATGAAGTCGTATAAGGGGATTTTCCCATCCCCTTAATTTCAAAGCATTATAAAAGCATTGGTAACAAGG
ATTAAATCATGATTGGTATT

Product: N-acetylgalactosamine-specific enzyme IID component of PTS

Products: protein histidine; sugar phosphate

Alternate protein names: EIID-Aga; PTS system N-acetylgalactosamine-specific EIID component [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MVSKTENPNALIDGIDEFQGTQVANPKSLVDGIDEYQDKEVRKVISKKDLWKCAIRGLFMEGNFNFERMQGGGFAYSIVP
ALKKIHGNNKRDLATATKNHLQFFNASPKLFTFLLGTAIAMEENKEKPSTVNVMKVAGMGPTGGIGDAIDHMTLMPLTLA
LGASIAMQGSIAGPFVFFILYQIVHFFVYFGLMFMGYKAGTAAMVNMSDATEKLAKAANIMGIFVIGALSASFIKVQTTL
SLELGGKVVELQTALFDKIMPNILPLALVFFMLKMVKGTGFWAKPPVLIFSTLAGGVIGHYLGIL

Sequences:

>Translated_305_residues
MVSKTENPNALIDGIDEFQGTQVANPKSLVDGIDEYQDKEVRKVISKKDLWKCAIRGLFMEGNFNFERMQGGGFAYSIVP
ALKKIHGNNKRDLATATKNHLQFFNASPKLFTFLLGTAIAMEENKEKPSTVNVMKVAGMGPTGGIGDAIDHMTLMPLTLA
LGASIAMQGSIAGPFVFFILYQIVHFFVYFGLMFMGYKAGTAAMVNMSDATEKLAKAANIMGIFVIGALSASFIKVQTTL
SLELGGKVVELQTALFDKIMPNILPLALVFFMLKMVKGTGFWAKPPVLIFSTLAGGVIGHYLGIL
>Mature_305_residues
MVSKTENPNALIDGIDEFQGTQVANPKSLVDGIDEYQDKEVRKVISKKDLWKCAIRGLFMEGNFNFERMQGGGFAYSIVP
ALKKIHGNNKRDLATATKNHLQFFNASPKLFTFLLGTAIAMEENKEKPSTVNVMKVAGMGPTGGIGDAIDHMTLMPLTLA
LGASIAMQGSIAGPFVFFILYQIVHFFVYFGLMFMGYKAGTAAMVNMSDATEKLAKAANIMGIFVIGALSASFIKVQTTL
SLELGGKVVELQTALFDKIMPNILPLALVFFMLKMVKGTGFWAKPPVLIFSTLAGGVIGHYLGIL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1789529, Length=265, Percent_Identity=38.8679245283019, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788122, Length=283, Percent_Identity=32.8621908127208, Blast_Score=151, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 33080; Mature: 33080

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKTENPNALIDGIDEFQGTQVANPKSLVDGIDEYQDKEVRKVISKKDLWKCAIRGLFM
CCCCCCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
EGNFNFERMQGGGFAYSIVPALKKIHGNNKRDLATATKNHLQFFNASPKLFTFLLGTAIA
ECCCCCEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MEENKEKPSTVNVMKVAGMGPTGGIGDAIDHMTLMPLTLALGASIAMQGSIAGPFVFFIL
HHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHH
YQIVHFFVYFGLMFMGYKAGTAAMVNMSDATEKLAKAANIMGIFVIGALSASFIKVQTTL
HHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
SLELGGKVVELQTALFDKIMPNILPLALVFFMLKMVKGTGFWAKPPVLIFSTLAGGVIGH
EEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YLGIL
HHHCC
>Mature Secondary Structure
MVSKTENPNALIDGIDEFQGTQVANPKSLVDGIDEYQDKEVRKVISKKDLWKCAIRGLFM
CCCCCCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
EGNFNFERMQGGGFAYSIVPALKKIHGNNKRDLATATKNHLQFFNASPKLFTFLLGTAIA
ECCCCCEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MEENKEKPSTVNVMKVAGMGPTGGIGDAIDHMTLMPLTLALGASIAMQGSIAGPFVFFIL
HHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHH
YQIVHFFVYFGLMFMGYKAGTAAMVNMSDATEKLAKAANIMGIFVIGALSASFIKVQTTL
HHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
SLELGGKVVELQTALFDKIMPNILPLALVFFMLKMVKGTGFWAKPPVLIFSTLAGGVIGH
EEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YLGIL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10931310; 9278503; 8932697 [H]