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The map label for this gene is sbmA [H]

Identifier: 59713856

GI number: 59713856

Start: 743673

End: 744692

Strand: Reverse

Name: sbmA [H]

Synonym: VF_A0673

Alternate gene names: 59713856

Gene position: 744692-743673 (Counterclockwise)

Preceding gene: 59713857

Following gene: 59713855

Centisome position: 55.98

GC content: 39.22

Gene sequence:

>1020_bases
ATGTTTCGTTGTTTCTTTTTAAATAGAAAATGGTTCTTTTGGTCAATTTACGGCACTACTGCGGTGGTTGCTGTTAATTG
GTTCAGAGTCGGTATTGATGTGGAACTTAACTACTGGAACGGTCATTTTTATGATCTTATCCAAGAGGCGTTAGTTTCTC
CCAATGCCGTCAAGTTTGTTGATTTTAGAAATGTTATTTTTGATTTTTTTAAGATCACAACTATGTACGTCGTTATTTCT
GTGGTGGTTGATTACTTCGTAAGGCACTGGATCTTTAGGTGGCGTTGCGCCATGAACGAGCACTATACAAAGCACTGGCC
TTTAATTCGTCAAATCGAAGGAGCAAGTCAGAGGATACAAGAAGACACAATGAGGTTTGCAAAGCTGGTTGAAGACTTAG
GAATAAGCATTCTTAGGAATTTCATGACACTAGCGACGTTTATCCCGTTGTTGTGGGAACTATCCAAGCACGTAAAGGTG
ATCCCGTTCTTTGGTGGGGTTGAACATTCAATGGTTATCGTTGCCGTTGTTTCCTCATTCGGTGGAATCGCGTTTTTAAT
GTTCATTGGTAGGAAATTACCAGCTTATGAATACAACAACCAAAAAGTAGAGGCGGCATTCAGGAAAGAATTAGTTTTAG
GGGAAGATGATGAAAGCCGAGCTACACCGAAAGGCTTAAAAGAACTGTTTGATGATGTGCGCCACAACTATTTTAGTATG
TATCGTGCTTATCTTTGGTTTGATAGCTGTTCAAGTGCATATAGCAAGTATTCGGAACTTACCGTTTACATTTTTATGAG
TCCAACAATATTACAAGCGGCGATTACCTTGGGAGTGTTGAAGCAAATCACTTCATCATACTCAAAGGTTGAGAGTTCCT
TAAGGTTCCTAATCCGCCGATGGTCGAGCTGTGTCGAGTTAATGTCAATTTACAAGCGCCTACGCGCTTTTGAAATGCAA
ATAGAAATTTTCTCTTTAGAAGAAAACTCGAATTTAACCTTAAAAGGCAAAAAAATATGA

Upstream 100 bases:

>100_bases
TACCAGCAATGGGATAATCGTTTATTTCGTATGGAACCAGTGATTATTAAAAACTAATAAAAGCAATAAAAGGTAACTTT
ATTAATTGAGGTTATCGCTT

Downstream 100 bases:

>100_bases
CCAAAACTAAATTAACCACTCTTGCTATTGCTATTGCTGCGTCATTATCGCCCGTTAAATCTTTTGCTGATACAGATAAT
GTTTCTACGTTTGAAGCCAT

Product: putative transporter

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MFRCFFLNRKWFFWSIYGTTAVVAVNWFRVGIDVELNYWNGHFYDLIQEALVSPNAVKFVDFRNVIFDFFKITTMYVVIS
VVVDYFVRHWIFRWRCAMNEHYTKHWPLIRQIEGASQRIQEDTMRFAKLVEDLGISILRNFMTLATFIPLLWELSKHVKV
IPFFGGVEHSMVIVAVVSSFGGIAFLMFIGRKLPAYEYNNQKVEAAFRKELVLGEDDESRATPKGLKELFDDVRHNYFSM
YRAYLWFDSCSSAYSKYSELTVYIFMSPTILQAAITLGVLKQITSSYSKVESSLRFLIRRWSSCVELMSIYKRLRAFEMQ
IEIFSLEENSNLTLKGKKI

Sequences:

>Translated_339_residues
MFRCFFLNRKWFFWSIYGTTAVVAVNWFRVGIDVELNYWNGHFYDLIQEALVSPNAVKFVDFRNVIFDFFKITTMYVVIS
VVVDYFVRHWIFRWRCAMNEHYTKHWPLIRQIEGASQRIQEDTMRFAKLVEDLGISILRNFMTLATFIPLLWELSKHVKV
IPFFGGVEHSMVIVAVVSSFGGIAFLMFIGRKLPAYEYNNQKVEAAFRKELVLGEDDESRATPKGLKELFDDVRHNYFSM
YRAYLWFDSCSSAYSKYSELTVYIFMSPTILQAAITLGVLKQITSSYSKVESSLRFLIRRWSSCVELMSIYKRLRAFEMQ
IEIFSLEENSNLTLKGKKI
>Mature_339_residues
MFRCFFLNRKWFFWSIYGTTAVVAVNWFRVGIDVELNYWNGHFYDLIQEALVSPNAVKFVDFRNVIFDFFKITTMYVVIS
VVVDYFVRHWIFRWRCAMNEHYTKHWPLIRQIEGASQRIQEDTMRFAKLVEDLGISILRNFMTLATFIPLLWELSKHVKV
IPFFGGVEHSMVIVAVVSSFGGIAFLMFIGRKLPAYEYNNQKVEAAFRKELVLGEDDESRATPKGLKELFDDVRHNYFSM
YRAYLWFDSCSSAYSKYSELTVYIFMSPTILQAAITLGVLKQITSSYSKVESSLRFLIRRWSSCVELMSIYKRLRAFEMQ
IEIFSLEENSNLTLKGKKI

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=331, Percent_Identity=44.1087613293051, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1787772, Length=317, Percent_Identity=22.7129337539432, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 39945; Mature: 39945

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRCFFLNRKWFFWSIYGTTAVVAVNWFRVGIDVELNYWNGHFYDLIQEALVSPNAVKFV
CEEEEEECCCEEEEEEECCCEEEEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEHH
DFRNVIFDFFKITTMYVVISVVVDYFVRHWIFRWRCAMNEHYTKHWPLIRQIEGASQRIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHH
EDTMRFAKLVEDLGISILRNFMTLATFIPLLWELSKHVKVIPFFGGVEHSMVIVAVVSSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHH
GGIAFLMFIGRKLPAYEYNNQKVEAAFRKELVLGEDDESRATPKGLKELFDDVRHNYFSM
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRAYLWFDSCSSAYSKYSELTVYIFMSPTILQAAITLGVLKQITSSYSKVESSLRFLIRR
HHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSCVELMSIYKRLRAFEMQIEIFSLEENSNLTLKGKKI
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCC
>Mature Secondary Structure
MFRCFFLNRKWFFWSIYGTTAVVAVNWFRVGIDVELNYWNGHFYDLIQEALVSPNAVKFV
CEEEEEECCCEEEEEEECCCEEEEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEHH
DFRNVIFDFFKITTMYVVISVVVDYFVRHWIFRWRCAMNEHYTKHWPLIRQIEGASQRIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHH
EDTMRFAKLVEDLGISILRNFMTLATFIPLLWELSKHVKVIPFFGGVEHSMVIVAVVSSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHH
GGIAFLMFIGRKLPAYEYNNQKVEAAFRKELVLGEDDESRATPKGLKELFDDVRHNYFSM
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRAYLWFDSCSSAYSKYSELTVYIFMSPTILQAAITLGVLKQITSSYSKVESSLRFLIRR
HHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSCVELMSIYKRLRAFEMQIEIFSLEENSNLTLKGKKI
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]