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The map label for this gene is kbl [C]

Identifier: 59713601

GI number: 59713601

Start: 472244

End: 473440

Strand: Reverse

Name: kbl [C]

Synonym: VF_A0418

Alternate gene names: 59713601

Gene position: 473440-472244 (Counterclockwise)

Preceding gene: 172087804

Following gene: 59713600

Centisome position: 35.59

GC content: 41.52

Gene sequence:

>1197_bases
ATGTCTTCTGCATTCTACACACAGATTCAAAACCAAATTGAAGAAGTAAAAAACGAAGGTTTATATAAATCAGAACGTAT
TATTACTTCAGCTCAAAAAGCATCAGTTTCTATTTCTACTGGCCAAGAAGTACTAAATTTCTGTGCAAATAACTACTTAG
GTTTAGCTAACCACCCTGCTCTTATTGAAGCAGCAAAAGATGGAATGGATGAGCATGGCTTTGGTATGGCATCAGTTCGT
TTTATTTGTGGTACTCAAGATTCACATAAAGAATTAGAACAAAAGCTATCTACTTTCTTAGGTAAAGAAGACACTATTCT
TTACACATCATGTTTTGATGCAAATGCTGGTTTGTTTGAAACCATTCTAGGTGCAGAAGATGCGATTATCTCAGATGCAC
TAAACCACGCATCAATCATTGATGGTGTGCGTCTATGTAAAGCAATGCGTTTCCGCTACGCAAACAACAACATGACTGAA
CTTGAAGAGCAATTAATTGCAGCAAAAGAAGCTGGCGCTCGTCATACGCTTATCGTAACTGATGGTGTGTTCTCAATGGA
TGGCGTTGTTGCAAACCTTCCTGCAATCTGTGACTTAGCAGATAAATACGACGCCCTTGTGATGGTTGATGACTCTCACG
CAGTTGGCTTTATGGGTGAAAACGGTGCTGGTACTCATGAGTATCACGATGTAATCGACCGTATCGATATCATCACAGGC
ACACTTGGTAAAGCAATGGGTGGCGCATCAGGCGGCTACACTTCAGGTAAAAAAGAAGTGATTGACTGGCTTCGTCAGCG
TTCTCGCCCTTACCTATTCTCAAACTCTGTTGCTCCGGCCATTGTATCTGCATCTATTCGTGTTTTAGATCTTCTGGCTG
AATCAGGTGACCTGCGTTCTAAATTATGGGAGAACTCAGCGCATTTCCGTACACGTATGGAAGCAGCAGGCTTTACTATG
GGCGGTGCTGATCACGCAATTATTCCAATCATGCTTGGAGATGCAAAAATCGCTGTTGAGTTTGCTGAACGTGCACTAGA
AAAAGGGATTTATGTTATTGCATTCTCTTTCCCAGTAGTACCAAAAGGCCAAGCTCGTATTCGTACACAAATGTCCGCTG
CTCACACTCGTGAACAGCTAGATCGTGCAATTGATGCCTTTATCGAAGTAGGTAAAGACATGGAGATCATTAAATAA

Upstream 100 bases:

>100_bases
TTTAGGGATAATAGTCGTAATTGATTTGAATTAGAATCTATTTATCGGATTTTGATTACACTTTTACGTGCAACAAACAT
AACTAAAAAAAGGAACCGCA

Downstream 100 bases:

>100_bases
TGAAAATTAAAGCGCTTTCAAAATTAAAACCTGAAGAAGGTATCTGGCTAACTGAGGTTGAAAAACCTGAGATGGGTCAT
AACGATCTTCTTATCCGCAT

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSSAFYTQIQNQIEEVKNEGLYKSERIITSAQKASVSISTGQEVLNFCANNYLGLANHPALIEAAKDGMDEHGFGMASVR
FICGTQDSHKELEQKLSTFLGKEDTILYTSCFDANAGLFETILGAEDAIISDALNHASIIDGVRLCKAMRFRYANNNMTE
LEEQLIAAKEAGARHTLIVTDGVFSMDGVVANLPAICDLADKYDALVMVDDSHAVGFMGENGAGTHEYHDVIDRIDIITG
TLGKAMGGASGGYTSGKKEVIDWLRQRSRPYLFSNSVAPAIVSASIRVLDLLAESGDLRSKLWENSAHFRTRMEAAGFTM
GGADHAIIPIMLGDAKIAVEFAERALEKGIYVIAFSFPVVPKGQARIRTQMSAAHTREQLDRAIDAFIEVGKDMEIIK

Sequences:

>Translated_398_residues
MSSAFYTQIQNQIEEVKNEGLYKSERIITSAQKASVSISTGQEVLNFCANNYLGLANHPALIEAAKDGMDEHGFGMASVR
FICGTQDSHKELEQKLSTFLGKEDTILYTSCFDANAGLFETILGAEDAIISDALNHASIIDGVRLCKAMRFRYANNNMTE
LEEQLIAAKEAGARHTLIVTDGVFSMDGVVANLPAICDLADKYDALVMVDDSHAVGFMGENGAGTHEYHDVIDRIDIITG
TLGKAMGGASGGYTSGKKEVIDWLRQRSRPYLFSNSVAPAIVSASIRVLDLLAESGDLRSKLWENSAHFRTRMEAAGFTM
GGADHAIIPIMLGDAKIAVEFAERALEKGIYVIAFSFPVVPKGQARIRTQMSAAHTREQLDRAIDAFIEVGKDMEIIK
>Mature_397_residues
SSAFYTQIQNQIEEVKNEGLYKSERIITSAQKASVSISTGQEVLNFCANNYLGLANHPALIEAAKDGMDEHGFGMASVRF
ICGTQDSHKELEQKLSTFLGKEDTILYTSCFDANAGLFETILGAEDAIISDALNHASIIDGVRLCKAMRFRYANNNMTEL
EEQLIAAKEAGARHTLIVTDGVFSMDGVVANLPAICDLADKYDALVMVDDSHAVGFMGENGAGTHEYHDVIDRIDIITGT
LGKAMGGASGGYTSGKKEVIDWLRQRSRPYLFSNSVAPAIVSASIRVLDLLAESGDLRSKLWENSAHFRTRMEAAGFTMG
GADHAIIPIMLGDAKIAVEFAERALEKGIYVIAFSFPVVPKGQARIRTQMSAAHTREQLDRAIDAFIEVGKDMEIIK

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=385, Percent_Identity=52.7272727272727, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI284448556, Length=411, Percent_Identity=49.6350364963504, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI83977442, Length=394, Percent_Identity=32.2335025380711, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI83977440, Length=394, Percent_Identity=32.2335025380711, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI83977444, Length=394, Percent_Identity=32.2335025380711, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI119220554, Length=371, Percent_Identity=34.7708894878706, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI4758668, Length=370, Percent_Identity=35.6756756756757, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI4502025, Length=400, Percent_Identity=31, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI40316939, Length=400, Percent_Identity=31, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI5454084, Length=389, Percent_Identity=25.4498714652956, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790046, Length=397, Percent_Identity=71.7884130982368, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=33.0508474576271, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71994529, Length=399, Percent_Identity=54.3859649122807, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=32.3369565217391, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=31.404958677686, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=31.404958677686, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71982625, Length=376, Percent_Identity=23.1382978723404, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982617, Length=376, Percent_Identity=23.1382978723404, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320438, Length=382, Percent_Identity=30.8900523560209, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=30.4347826086957, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323954, Length=266, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24662918, Length=389, Percent_Identity=56.2982005141388, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17137420, Length=349, Percent_Identity=31.8051575931232, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17136286, Length=376, Percent_Identity=33.5106382978723, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24653280, Length=362, Percent_Identity=23.2044198895028, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653276, Length=362, Percent_Identity=23.2044198895028, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653278, Length=362, Percent_Identity=23.2044198895028, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43315; Mature: 43184

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAFYTQIQNQIEEVKNEGLYKSERIITSAQKASVSISTGQEVLNFCANNYLGLANHPA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCCCCCCH
LIEAAKDGMDEHGFGMASVRFICGTQDSHKELEQKLSTFLGKEDTILYTSCFDANAGLFE
HHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
TILGAEDAIISDALNHASIIDGVRLCKAMRFRYANNNMTELEEQLIAAKEAGARHTLIVT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
DGVFSMDGVVANLPAICDLADKYDALVMVDDSHAVGFMGENGAGTHEYHDVIDRIDIITG
CCCCCCCCHHHCCCHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHH
TLGKAMGGASGGYTSGKKEVIDWLRQRSRPYLFSNSVAPAIVSASIRVLDLLAESGDLRS
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
KLWENSAHFRTRMEAAGFTMGGADHAIIPIMLGDAKIAVEFAERALEKGIYVIAFSFPVV
HHHCCCHHHHHHHHHCCCEECCCCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCC
PKGQARIRTQMSAAHTREQLDRAIDAFIEVGKDMEIIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
>Mature Secondary Structure 
SSAFYTQIQNQIEEVKNEGLYKSERIITSAQKASVSISTGQEVLNFCANNYLGLANHPA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCCCCCCH
LIEAAKDGMDEHGFGMASVRFICGTQDSHKELEQKLSTFLGKEDTILYTSCFDANAGLFE
HHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
TILGAEDAIISDALNHASIIDGVRLCKAMRFRYANNNMTELEEQLIAAKEAGARHTLIVT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
DGVFSMDGVVANLPAICDLADKYDALVMVDDSHAVGFMGENGAGTHEYHDVIDRIDIITG
CCCCCCCCHHHCCCHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHH
TLGKAMGGASGGYTSGKKEVIDWLRQRSRPYLFSNSVAPAIVSASIRVLDLLAESGDLRS
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
KLWENSAHFRTRMEAAGFTMGGADHAIIPIMLGDAKIAVEFAERALEKGIYVIAFSFPVV
HHHCCCHHHHHHHHHCCCEECCCCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCC
PKGQARIRTQMSAAHTREQLDRAIDAFIEVGKDMEIIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]