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The map label for this gene is pyrC [H]

Identifier: 59713595

GI number: 59713595

Start: 465059

End: 466087

Strand: Reverse

Name: pyrC [H]

Synonym: VF_A0412

Alternate gene names: 59713595

Gene position: 466087-465059 (Counterclockwise)

Preceding gene: 59713599

Following gene: 59713593

Centisome position: 35.04

GC content: 40.04

Gene sequence:

>1029_bases
ATGACAACTTTAACAATTACTCGTCCTGATGATTGGCATTTACACTTACGTGATGGTGATGTGTTAACTGACACAGTTCG
TGACTCTGGTCGTTACAACGGCAGAGCTTTAATCATGCCAAACCTTGTACCACCTGTAACAACTACCGAACAAGCATTAA
GTTACCGTGAACGCATTCAGGCTGAAAACAAATCTGATTCTTTTGCTCCTCTTATGTCTCTTTATCTAACAGAAAAGACA
ACATCAGAAGAGATCCGAAAAGCGAAAGCGACTGGACATATCGTAGCAGCAAAACTGTATCCAGCTGGTGCTACAACAAA
CTCAGACTCTGGTGTAAGCGACGTTAAAAACGTTTACCCTATTCTAAAAACGATGCAAGAAGAAGGCATGCTTTTACTTA
TTCATGGGGAAGTAACAACTCATGATATTGATATCTTCGATCGTGAAAAGACTTTCCTTGATACAGTTTTAGCGCCTATC
GTTAATGATTTTCCTGAGCTAAAAATTGTACTTGAGCACATCACAACTAAAGATGCGGCAGATTTTGTTAAAAACGCAGG
TCCAAACGTTGCTGCAACCATTACAGCGCATCACTTATTGTTTAACCGTAACCACATGTTAGTTGGTGGTATTAAACCTC
ACTTCTACTGTTTACCAATTTTAAAACGCAATACACACCAACAAGCTCTAGTGGAAGCTGCAACAAGCGGTAATCCTAAA
TTTTTCTTAGGTACTGATTCAGCACCTCATGCAAAAGACAAAAAAGAAGCGGCTTGTGGTTGTGCTGGTTCATATACTGC
CCACGCTTCAATCGAGCTTTATGCAGAAGTATTTGAAAACGAAGGTAAGTTAGAAAACCTAGAAGCATTCGCAAGCTTTA
ATGGTCCAGATTTTTATAACCTACCTCGCAACACTGACACAATAACGCTTGTAAAAGAAGCGTGGATTGCACCAGAGACA
ATGGCATTTGGAAATGACTTTGTTGTACCAATTCGTGCTGGTGAAGCTGTTGAGTGGCTAGTAAAGTAA

Upstream 100 bases:

>100_bases
TCGTTCTCTTTTATTTTGCTTTAGAATCCTACCGCCTAAGCAAACGATTAACTTCATTATTTAGGCTCCTTAATTATTCT
TTTACTGGTGGGAGCAGCAA

Downstream 100 bases:

>100_bases
GTCATAGATAAAATAAGCTAACAATAAAAATGGGATGCCAAATTGCATCCCATTTTTGTTTCTTACTTAATCACAGAAGA
GCTCTACGGCACACCGTGAC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTTLTITRPDDWHLHLRDGDVLTDTVRDSGRYNGRALIMPNLVPPVTTTEQALSYRERIQAENKSDSFAPLMSLYLTEKT
TSEEIRKAKATGHIVAAKLYPAGATTNSDSGVSDVKNVYPILKTMQEEGMLLLIHGEVTTHDIDIFDREKTFLDTVLAPI
VNDFPELKIVLEHITTKDAADFVKNAGPNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALVEAATSGNPK
FFLGTDSAPHAKDKKEAACGCAGSYTAHASIELYAEVFENEGKLENLEAFASFNGPDFYNLPRNTDTITLVKEAWIAPET
MAFGNDFVVPIRAGEAVEWLVK

Sequences:

>Translated_342_residues
MTTLTITRPDDWHLHLRDGDVLTDTVRDSGRYNGRALIMPNLVPPVTTTEQALSYRERIQAENKSDSFAPLMSLYLTEKT
TSEEIRKAKATGHIVAAKLYPAGATTNSDSGVSDVKNVYPILKTMQEEGMLLLIHGEVTTHDIDIFDREKTFLDTVLAPI
VNDFPELKIVLEHITTKDAADFVKNAGPNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALVEAATSGNPK
FFLGTDSAPHAKDKKEAACGCAGSYTAHASIELYAEVFENEGKLENLEAFASFNGPDFYNLPRNTDTITLVKEAWIAPET
MAFGNDFVVPIRAGEAVEWLVK
>Mature_341_residues
TTLTITRPDDWHLHLRDGDVLTDTVRDSGRYNGRALIMPNLVPPVTTTEQALSYRERIQAENKSDSFAPLMSLYLTEKTT
SEEIRKAKATGHIVAAKLYPAGATTNSDSGVSDVKNVYPILKTMQEEGMLLLIHGEVTTHDIDIFDREKTFLDTVLAPIV
NDFPELKIVLEHITTKDAADFVKNAGPNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALVEAATSGNPKF
FLGTDSAPHAKDKKEAACGCAGSYTAHASIELYAEVFENEGKLENLEAFASFNGPDFYNLPRNTDTITLVKEAWIAPETM
AFGNDFVVPIRAGEAVEWLVK

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=340, Percent_Identity=54.1176470588235, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323452, Length=357, Percent_Identity=37.8151260504202, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 37794; Mature: 37663

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTITRPDDWHLHLRDGDVLTDTVRDSGRYNGRALIMPNLVPPVTTTEQALSYRERIQ
CCEEEEECCCCCEEEEECCCEEEHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
AENKSDSFAPLMSLYLTEKTTSEEIRKAKATGHIVAAKLYPAGATTNSDSGVSDVKNVYP
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
ILKTMQEEGMLLLIHGEVTTHDIDIFDREKTFLDTVLAPIVNDFPELKIVLEHITTKDAA
HHHHHHHCCEEEEEECCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
DFVKNAGPNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALVEAATSGNPK
HHHHCCCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCE
FFLGTDSAPHAKDKKEAACGCAGSYTAHASIELYAEVFENEGKLENLEAFASFNGPDFYN
EEEECCCCCCCCCCHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
LPRNTDTITLVKEAWIAPETMAFGNDFVVPIRAGEAVEWLVK
CCCCCCEEEEEEHHHCCCCCEECCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure 
TTLTITRPDDWHLHLRDGDVLTDTVRDSGRYNGRALIMPNLVPPVTTTEQALSYRERIQ
CEEEEECCCCCEEEEECCCEEEHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
AENKSDSFAPLMSLYLTEKTTSEEIRKAKATGHIVAAKLYPAGATTNSDSGVSDVKNVYP
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
ILKTMQEEGMLLLIHGEVTTHDIDIFDREKTFLDTVLAPIVNDFPELKIVLEHITTKDAA
HHHHHHHCCEEEEEECCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
DFVKNAGPNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALVEAATSGNPK
HHHHCCCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCE
FFLGTDSAPHAKDKKEAACGCAGSYTAHASIELYAEVFENEGKLENLEAFASFNGPDFYN
EEEECCCCCCCCCCHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
LPRNTDTITLVKEAWIAPETMAFGNDFVVPIRAGEAVEWLVK
CCCCCCEEEEEEHHHCCCCCEECCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA