Click here to switch to the map view.

The map label for this gene is yhcK [H]

Identifier: 59713581

GI number: 59713581

Start: 451254

End: 452837

Strand: Reverse

Name: yhcK [H]

Synonym: VF_A0398

Alternate gene names: 59713581

Gene position: 452837-451254 (Counterclockwise)

Preceding gene: 59713582

Following gene: 59713578

Centisome position: 34.04

GC content: 34.34

Gene sequence:

>1584_bases
ATGGACAGTCTTTTAAATCGAATAGCTGAAAATAACATTAATTTATTAAAGCTAAACACACACCAGTGCCTAGAGTTTTG
GTTATATATTGTAGACAACTTCGCTGAAACAAAAACCGAAAAAGCATATGCCCTATTAATGAGTTCTGAGTATGAGGTCG
AATTCAATAACCTGACAAACAGCATCAAACATTTACACCGTGCTTTATTGTTATTAGATCCATCTAAAGACATCGAATTA
ATGCTGCAAATATATTCGGCATTAAGTTATCGTTATACTGATATTGGACAGTACCAAAAAGCATTAAAGTACCTTCATTC
GCTTTCTGAACTCGCCGTAGAATACGGTGATAATGAATTTTATATACAAGCTATTTTAGGTATAGGAAATTTATGCTCTA
TTTATGGTGATCACCTAAAAGCACTGCGCTATTACCAAAAGTTAGAAGCGTTAAGCAACAGTATTAATAGCAATAATCTC
TCGCTTCGTTACCGACTTTACATGATTGCTTGCCTTCTCGATTTAAAAAGACTTTCCAAAGCTAAAGAGCTTCTTGATGA
ATGTTATGCTTTAAAATACGTTTCTGATGACCCCCAATTAACAGCGCAAGTATTGCTTTATTCCGCAAAACTATTGCGCC
TGAAAAACCAACCTGAAACGGCACTTAACCTACTGATACAATATAGACAAGAGTATAGCGACCAACACTCTTTTCCTTGG
TTAAACAAGTTATTTGCTGTTGAATCAGCCTACTGTCTTATTAAGCAACAGCGTGGGGAAATTGCCGATGTGATCATTTC
ACATCAATTAAAGCGAACAATAAAATACTCTTATGGCTACTATATTCGCCAATTACTTGAAGTGAAAAGCGATGCTCTAG
CAAGTTGTAAAAACTTTGCTCTTGCACTTGAATGTGAAAAAAAAGCGCAGCAGTTAACTGTCGAAATAATCAAAAACTTC
CCAATTAATGAACTTGGTGGTCACTCTTTAAGACGATTAACTCGATTAGAACTTCAATTACGCTTAAATATTTCTGAAAC
TGAAAACTTAAAATTAAAAAAAGTATCTGATCAACAAAAAGACACGGTAGCCAAGTTACAACAAGATGTTTTTCATGACA
GTTTAACGAAACTGTATAACCGAAGATGGTTTGAATCTACGTTTATAAAAGAGATTTTACCTACGATTAAACACTATCAG
TTATTGGTTATTGATATCGATGATTTTAAATCAATAAATGATGAATATTCCCACTTAAAAGGCGATATTGTATTAAAACT
AGTTGGTAAAATTTTGCAAAATTCAGTTTCACCTTCTCACTATACACTACGTTATGGAGGAGAAGAGTTTCTTATTATTA
TTCCTTCTGGCGACCTTGAGCATGGGAAAAAAATCGCAGAACAATGCCGATCAGAAATTGCAAATTACGATTGGCGAGAT
ACCCTTAAAGACCGCTCAATTACCGTTAGTATCGGCTTAACATTGAATCGAAATAAAGAAGATCATAAAGCTACGTTTCT
TCGTGCAGATAAAGCGCTTTACCAAGCAAAACGCTCCGGAAAGAATAAAGTATGTTCTTATTAA

Upstream 100 bases:

>100_bases
CGTTACTCTTTTGAGGCATCTTCCCCAATGATTAACTATTTCACAATTACGGAATATTTTGCGACGGTGCGGCCTTCTCA
AACACCAATGGAAATATGAT

Downstream 100 bases:

>100_bases
ACAAATAACTTTTATTAAGATATAAAAAAAGCTAGCAAACTTGCTAGCTTTTTCCATTCGTCTATATCTTAACGATAAAG
ACCTAAATCTTTTTGTAAGT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDSLLNRIAENNINLLKLNTHQCLEFWLYIVDNFAETKTEKAYALLMSSEYEVEFNNLTNSIKHLHRALLLLDPSKDIEL
MLQIYSALSYRYTDIGQYQKALKYLHSLSELAVEYGDNEFYIQAILGIGNLCSIYGDHLKALRYYQKLEALSNSINSNNL
SLRYRLYMIACLLDLKRLSKAKELLDECYALKYVSDDPQLTAQVLLYSAKLLRLKNQPETALNLLIQYRQEYSDQHSFPW
LNKLFAVESAYCLIKQQRGEIADVIISHQLKRTIKYSYGYYIRQLLEVKSDALASCKNFALALECEKKAQQLTVEIIKNF
PINELGGHSLRRLTRLELQLRLNISETENLKLKKVSDQQKDTVAKLQQDVFHDSLTKLYNRRWFESTFIKEILPTIKHYQ
LLVIDIDDFKSINDEYSHLKGDIVLKLVGKILQNSVSPSHYTLRYGGEEFLIIIPSGDLEHGKKIAEQCRSEIANYDWRD
TLKDRSITVSIGLTLNRNKEDHKATFLRADKALYQAKRSGKNKVCSY

Sequences:

>Translated_527_residues
MDSLLNRIAENNINLLKLNTHQCLEFWLYIVDNFAETKTEKAYALLMSSEYEVEFNNLTNSIKHLHRALLLLDPSKDIEL
MLQIYSALSYRYTDIGQYQKALKYLHSLSELAVEYGDNEFYIQAILGIGNLCSIYGDHLKALRYYQKLEALSNSINSNNL
SLRYRLYMIACLLDLKRLSKAKELLDECYALKYVSDDPQLTAQVLLYSAKLLRLKNQPETALNLLIQYRQEYSDQHSFPW
LNKLFAVESAYCLIKQQRGEIADVIISHQLKRTIKYSYGYYIRQLLEVKSDALASCKNFALALECEKKAQQLTVEIIKNF
PINELGGHSLRRLTRLELQLRLNISETENLKLKKVSDQQKDTVAKLQQDVFHDSLTKLYNRRWFESTFIKEILPTIKHYQ
LLVIDIDDFKSINDEYSHLKGDIVLKLVGKILQNSVSPSHYTLRYGGEEFLIIIPSGDLEHGKKIAEQCRSEIANYDWRD
TLKDRSITVSIGLTLNRNKEDHKATFLRADKALYQAKRSGKNKVCSY
>Mature_527_residues
MDSLLNRIAENNINLLKLNTHQCLEFWLYIVDNFAETKTEKAYALLMSSEYEVEFNNLTNSIKHLHRALLLLDPSKDIEL
MLQIYSALSYRYTDIGQYQKALKYLHSLSELAVEYGDNEFYIQAILGIGNLCSIYGDHLKALRYYQKLEALSNSINSNNL
SLRYRLYMIACLLDLKRLSKAKELLDECYALKYVSDDPQLTAQVLLYSAKLLRLKNQPETALNLLIQYRQEYSDQHSFPW
LNKLFAVESAYCLIKQQRGEIADVIISHQLKRTIKYSYGYYIRQLLEVKSDALASCKNFALALECEKKAQQLTVEIIKNF
PINELGGHSLRRLTRLELQLRLNISETENLKLKKVSDQQKDTVAKLQQDVFHDSLTKLYNRRWFESTFIKEILPTIKHYQ
LLVIDIDDFKSINDEYSHLKGDIVLKLVGKILQNSVSPSHYTLRYGGEEFLIIIPSGDLEHGKKIAEQCRSEIANYDWRD
TLKDRSITVSIGLTLNRNKEDHKATFLRADKALYQAKRSGKNKVCSY

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=35.2601156069364, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.1851851851852, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=32.7272727272727, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=31.9767441860465, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=33.5443037974684, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=159, Percent_Identity=32.7044025157233, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=28.021978021978, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=154, Percent_Identity=31.1688311688312, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=196, Percent_Identity=30.6122448979592, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=155, Percent_Identity=31.6129032258064, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 61223; Mature: 61223

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLLNRIAENNINLLKLNTHQCLEFWLYIVDNFAETKTEKAYALLMSSEYEVEFNNLTN
CCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHH
SIKHLHRALLLLDPSKDIELMLQIYSALSYRYTDIGQYQKALKYLHSLSELAVEYGDNEF
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YIQAILGIGNLCSIYGDHLKALRYYQKLEALSNSINSNNLSLRYRLYMIACLLDLKRLSK
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHH
AKELLDECYALKYVSDDPQLTAQVLLYSAKLLRLKNQPETALNLLIQYRQEYSDQHSFPW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCH
LNKLFAVESAYCLIKQQRGEIADVIISHQLKRTIKYSYGYYIRQLLEVKSDALASCKNFA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALECEKKAQQLTVEIIKNFPINELGGHSLRRLTRLELQLRLNISETENLKLKKVSDQQK
EEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCEEEEECCCHHH
DTVAKLQQDVFHDSLTKLYNRRWFESTFIKEILPTIKHYQLLVIDIDDFKSINDEYSHLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCHHHHHHH
GDIVLKLVGKILQNSVSPSHYTLRYGGEEFLIIIPSGDLEHGKKIAEQCRSEIANYDWRD
HHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHH
TLKDRSITVSIGLTLNRNKEDHKATFLRADKALYQAKRSGKNKVCSY
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDSLLNRIAENNINLLKLNTHQCLEFWLYIVDNFAETKTEKAYALLMSSEYEVEFNNLTN
CCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHH
SIKHLHRALLLLDPSKDIELMLQIYSALSYRYTDIGQYQKALKYLHSLSELAVEYGDNEF
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YIQAILGIGNLCSIYGDHLKALRYYQKLEALSNSINSNNLSLRYRLYMIACLLDLKRLSK
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHH
AKELLDECYALKYVSDDPQLTAQVLLYSAKLLRLKNQPETALNLLIQYRQEYSDQHSFPW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCH
LNKLFAVESAYCLIKQQRGEIADVIISHQLKRTIKYSYGYYIRQLLEVKSDALASCKNFA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALECEKKAQQLTVEIIKNFPINELGGHSLRRLTRLELQLRLNISETENLKLKKVSDQQK
EEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCEEEEECCCHHH
DTVAKLQQDVFHDSLTKLYNRRWFESTFIKEILPTIKHYQLLVIDIDDFKSINDEYSHLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCHHHHHHH
GDIVLKLVGKILQNSVSPSHYTLRYGGEEFLIIIPSGDLEHGKKIAEQCRSEIANYDWRD
HHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHH
TLKDRSITVSIGLTLNRNKEDHKATFLRADKALYQAKRSGKNKVCSY
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]