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The map label for this gene is sapB [H]

Identifier: 59713491

GI number: 59713491

Start: 342287

End: 343234

Strand: Reverse

Name: sapB [H]

Synonym: VF_A0308

Alternate gene names: 59713491

Gene position: 343234-342287 (Counterclockwise)

Preceding gene: 59713492

Following gene: 59713490

Centisome position: 25.8

GC content: 37.45

Gene sequence:

>948_bases
ATGTGGATTTATACCATTAGACGCTTTAATTTATTTGTTATTACCTTACTCATTTTAACTCTGATTGGTTTTAATATTTT
ACGCCTAGATGATACATCTGCTTGGGCGCAAACGCCTTTTTTATCTGGTTGGTTAACTTATCTTGGTCAACTATTAAACG
GTAATTTAGGAGTTAATCAATATGGCGTAAAAATGCTTAATGAAATACTTCGAGTCTTCCCTGCGACTATCGAACTTTGT
TTCTTTGCTTTCTTTATTGCACTGATTATAGGTACGCCGATTGGTACTATTGCCGGAATGCGTAGAGGTAAGCCTTTAGA
TACACTAATCTCATCAATCGCATTAGTTGGTTATTCCATGCCTTTATTCTGGATTGCTTTATTAGGTTTACAACTAGGCG
TAACTCCAGTTTCAGGTCGTTACAGCTTATTGTATGAATTCGATGCTAAAACAGGGTTTGCCTTTATTGATGTGCTTGCC
TCAAAATCGCCTTATCGGGCAGAAATGCTTGAGAACATTATTCAGCATTTAATTTTACCCACATTAGTACTTTCCATTGC
GCCGATGACAGAAGTTGTTCGTTTAATGCGCTCGTCCGTCGCAACAGTTATTACACAAAACTACATCAAAGTTGCTCATA
CTAAAGGCCTGTCTAAATTTGAAATCATGGTCAAGCACATAATCCGTAATGCTTTACCTCCAATTATTCCAAAATTAGGA
GTTCAATTATCGGGCTTATTTACCGCGGCGATCTTAACTGAATCCATTTTTAATTGGCCAGGTATTGGGCACTGGCTACT
TGATGCCATTATTAATCAAGATTTTGTCGCCATTCAGGCTGGCGTTATAACTGTTGCGTCATTCATTTTATTAGCCAACA
TTTTATCTGATTTACTGGGTGCAGCAGTTAACCCGTTAGTAAGGAAAGAGTTCTATGCTCTCAAATAA

Upstream 100 bases:

>100_bases
TTAAATATCAAGCAAATCAATCTTCTTTAACAGGCTTCACATTAAGCCCTTTTAATACTTACTCTTTCCATAACGTTGTT
AGAGTTAAGGAGTTGAACTA

Downstream 100 bases:

>100_bases
TGTTTATCAAGAAGAACATATCCCTACTCAAATGGAACGTTTCTGGTTGAGTTATCGAGCGAATTCACTCGCGATGTTCG
GTTTTTGGTGCTTATTACTG

Product: peptide transport system permease protein SapB

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MWIYTIRRFNLFVITLLILTLIGFNILRLDDTSAWAQTPFLSGWLTYLGQLLNGNLGVNQYGVKMLNEILRVFPATIELC
FFAFFIALIIGTPIGTIAGMRRGKPLDTLISSIALVGYSMPLFWIALLGLQLGVTPVSGRYSLLYEFDAKTGFAFIDVLA
SKSPYRAEMLENIIQHLILPTLVLSIAPMTEVVRLMRSSVATVITQNYIKVAHTKGLSKFEIMVKHIIRNALPPIIPKLG
VQLSGLFTAAILTESIFNWPGIGHWLLDAIINQDFVAIQAGVITVASFILLANILSDLLGAAVNPLVRKEFYALK

Sequences:

>Translated_315_residues
MWIYTIRRFNLFVITLLILTLIGFNILRLDDTSAWAQTPFLSGWLTYLGQLLNGNLGVNQYGVKMLNEILRVFPATIELC
FFAFFIALIIGTPIGTIAGMRRGKPLDTLISSIALVGYSMPLFWIALLGLQLGVTPVSGRYSLLYEFDAKTGFAFIDVLA
SKSPYRAEMLENIIQHLILPTLVLSIAPMTEVVRLMRSSVATVITQNYIKVAHTKGLSKFEIMVKHIIRNALPPIIPKLG
VQLSGLFTAAILTESIFNWPGIGHWLLDAIINQDFVAIQAGVITVASFILLANILSDLLGAAVNPLVRKEFYALK
>Mature_315_residues
MWIYTIRRFNLFVITLLILTLIGFNILRLDDTSAWAQTPFLSGWLTYLGQLLNGNLGVNQYGVKMLNEILRVFPATIELC
FFAFFIALIIGTPIGTIAGMRRGKPLDTLISSIALVGYSMPLFWIALLGLQLGVTPVSGRYSLLYEFDAKTGFAFIDVLA
SKSPYRAEMLENIIQHLILPTLVLSIAPMTEVVRLMRSSVATVITQNYIKVAHTKGLSKFEIMVKHIIRNALPPIIPKLG
VQLSGLFTAAILTESIFNWPGIGHWLLDAIINQDFVAIQAGVITVASFILLANILSDLLGAAVNPLVRKEFYALK

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4168

COG function: function code V; ABC-type antimicrobial peptide transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787550, Length=319, Percent_Identity=47.6489028213166, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1789965, Length=283, Percent_Identity=36.7491166077738, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1787761, Length=249, Percent_Identity=36.144578313253, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787053, Length=275, Percent_Identity=28.3636363636364, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789888, Length=275, Percent_Identity=27.2727272727273, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787497, Length=329, Percent_Identity=24.9240121580547, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788504, Length=239, Percent_Identity=26.7782426778243, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34884; Mature: 34884

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIYTIRRFNLFVITLLILTLIGFNILRLDDTSAWAQTPFLSGWLTYLGQLLNGNLGVNQ
CEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCHH
YGVKMLNEILRVFPATIELCFFAFFIALIIGTPIGTIAGMRRGKPLDTLISSIALVGYSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
PLFWIALLGLQLGVTPVSGRYSLLYEFDAKTGFAFIDVLASKSPYRAEMLENIIQHLILP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLVLSIAPMTEVVRLMRSSVATVITQNYIKVAHTKGLSKFEIMVKHIIRNALPPIIPKLG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHC
VQLSGLFTAAILTESIFNWPGIGHWLLDAIINQDFVAIQAGVITVASFILLANILSDLLG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVNPLVRKEFYALK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MWIYTIRRFNLFVITLLILTLIGFNILRLDDTSAWAQTPFLSGWLTYLGQLLNGNLGVNQ
CEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCHH
YGVKMLNEILRVFPATIELCFFAFFIALIIGTPIGTIAGMRRGKPLDTLISSIALVGYSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
PLFWIALLGLQLGVTPVSGRYSLLYEFDAKTGFAFIDVLASKSPYRAEMLENIIQHLILP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLVLSIAPMTEVVRLMRSSVATVITQNYIKVAHTKGLSKFEIMVKHIIRNALPPIIPKLG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHC
VQLSGLFTAAILTESIFNWPGIGHWLLDAIINQDFVAIQAGVITVASFILLANILSDLLG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVNPLVRKEFYALK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]