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The map label for this gene is vpsT [H]

Identifier: 59713015

GI number: 59713015

Start: 2693255

End: 2693899

Strand: Reverse

Name: vpsT [H]

Synonym: VF_2408

Alternate gene names: 59713015

Gene position: 2693899-2693255 (Counterclockwise)

Preceding gene: 172087772

Following gene: 59713014

Centisome position: 92.97

GC content: 25.27

Gene sequence:

>645_bases
ATGAGTATTTATTATTTATCGTCAGATCCAACATTGTATTCATCATTACTTATTGATTTGTTGGAGAAAACCTCTGGTCG
AGCGATTATTTCTCTTAATTGTGATGAATTAGTAAATAAGGGGAAAATAGACGATGATATTCTAGTTATCTTGGATATAA
AAAAACAAACAGATAAAAAGTATAAAAATTATATAGATACTATTAATCAAAATAAATTAAAGGTAAGAGAAATACTTTTT
AATATAACTGATGAGAATATAAAAAAAAACATAATGAAATACCCAAATGTTGTAGGTATTTTTTATGAAAAAGATAATAT
GGATATTATATCTTATGGAGTAAAAAAAATAATAGAAGGAGAAATGTGGCTTAGTCGTAAAATAATTAGCGAGTTAATAT
TTACATATAGAGAAAAACAGAATGCAATATTGTCGACATCTATTTCATTAACGACAAGAGAGAGTGAAATATTAAGATTA
TTAGCATCTGGAGCTTCAAATTTAGATATCGCTAATTCATTGTTTGTAAGTGAAAATACAGTTAAAACGCATTTATATAA
TGTCTTTAAAAAATTAAATGTAAAAAATAGATTACAAGCAATGATGTGGGCTAAAGGGTATGACTTTGAAGGAAATAATC
GATGA

Upstream 100 bases:

>100_bases
AGTGATACATGTTGGGTATTATTAATAATTATTTAGTTATTCAGTGGATATGTAGAGGTTTTAAGCTAGTTTTAAATAAC
AGTCATCATGGAGGAGTGAG

Downstream 100 bases:

>100_bases
ATATTAAATATATTAGTATCGTTTTTTTTACATCTTTTATTTCTTTTGCTTCAGATAATAAAGCTTCGTTAGAGTCTAAA
GCTCCTTTGGAAAACAACAG

Product: DNA-binding transcriptional activator VpsT (CsgD-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSIYYLSSDPTLYSSLLIDLLEKTSGRAIISLNCDELVNKGKIDDDILVILDIKKQTDKKYKNYIDTINQNKLKVREILF
NITDENIKKNIMKYPNVVGIFYEKDNMDIISYGVKKIIEGEMWLSRKIISELIFTYREKQNAILSTSISLTTRESEILRL
LASGASNLDIANSLFVSENTVKTHLYNVFKKLNVKNRLQAMMWAKGYDFEGNNR

Sequences:

>Translated_214_residues
MSIYYLSSDPTLYSSLLIDLLEKTSGRAIISLNCDELVNKGKIDDDILVILDIKKQTDKKYKNYIDTINQNKLKVREILF
NITDENIKKNIMKYPNVVGIFYEKDNMDIISYGVKKIIEGEMWLSRKIISELIFTYREKQNAILSTSISLTTRESEILRL
LASGASNLDIANSLFVSENTVKTHLYNVFKKLNVKNRLQAMMWAKGYDFEGNNR
>Mature_213_residues
SIYYLSSDPTLYSSLLIDLLEKTSGRAIISLNCDELVNKGKIDDDILVILDIKKQTDKKYKNYIDTINQNKLKVREILFN
ITDENIKKNIMKYPNVVGIFYEKDNMDIISYGVKKIIEGEMWLSRKIISELIFTYREKQNAILSTSISLTTRESEILRLL
ASGASNLDIANSLFVSENTVKTHLYNVFKKLNVKNRLQAMMWAKGYDFEGNNR

Specific function: The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA t

COG id: COG2771

COG function: function code K; DNA-binding HTH domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787277, Length=170, Percent_Identity=38.8235294117647, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 24741; Mature: 24610

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYYLSSDPTLYSSLLIDLLEKTSGRAIISLNCDELVNKGKIDDDILVILDIKKQTDKK
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCEEEEEECCHHHHHH
YKNYIDTINQNKLKVREILFNITDENIKKNIMKYPNVVGIFYEKDNMDIISYGVKKIIEG
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCC
EMWLSRKIISELIFTYREKQNAILSTSISLTTRESEILRLLASGASNLDIANSLFVSENT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCHHHHEEECCCH
VKTHLYNVFKKLNVKNRLQAMMWAKGYDFEGNNR
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SIYYLSSDPTLYSSLLIDLLEKTSGRAIISLNCDELVNKGKIDDDILVILDIKKQTDKK
EEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCEEEEEECCHHHHHH
YKNYIDTINQNKLKVREILFNITDENIKKNIMKYPNVVGIFYEKDNMDIISYGVKKIIEG
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCC
EMWLSRKIISELIFTYREKQNAILSTSISLTTRESEILRLLASGASNLDIANSLFVSENT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCHHHHEEECCCH
VKTHLYNVFKKLNVKNRLQAMMWAKGYDFEGNNR
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8817489; 8905232; 9278503 [H]