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The map label for this gene is ghrA [H]

Identifier: 59713007

GI number: 59713007

Start: 2687259

End: 2688173

Strand: Direct

Name: ghrA [H]

Synonym: VF_2400

Alternate gene names: 59713007

Gene position: 2687259-2688173 (Clockwise)

Preceding gene: 59713005

Following gene: 59713011

Centisome position: 92.74

GC content: 37.27

Gene sequence:

>915_bases
ATGAATAAATTACTTATCATTTCAAAAGAAAAAGAGAAATACCTCTCACTTTTAGAGCAAGCGGATCTCCCGGATCTTGA
AATTTGTCACGATATTAGAAAAGCCGAGATCCTTCTTGCCGATCCACCACTGGTGATAGATCTCCTAGACCAATTCAAAA
ATCTAAAATGGTTACAGTCTACTTTTGCGGGTGTTGACTCTCTAATCCAACCTAATTTAAGAACCGATTACCATTTAACA
AATGTTCGTGGCATTTTCGGACAACCAATATCAGAGTATGTTTTGGGTTACTCCATTCAATACTTTCGTCATTTCAAACA
ATATGCTGAACAGCAGAAAAAGAAACAATGGCAGCCCCATACATACTCTTCATTAGTCAATAAGTGCATGGTTATTTTAG
GAACAGGTTCAATAGGAAGTGAGTTAGCCGCAACCGTTAAGCAACTTGGCTTTATCGTTTATGGTATTAACCGCTCAGGG
ATCCCTCCTAAAGATTCTCCCTTTGATTGTATATTCCATATTTCAGAAATTGAAAATGCTTTTCAATATGCAGATGTGAT
TGTAAGTACATTACCAAAGACTGAATTAACTATGGACTTACTAAATAGTCAAACCTTAAAAAAATGCAATGGTGCATTGC
TATTTAATGTTGGACGAGGAGAAGTACTGGAAAATTACGGTTTACTAAACGCTCTTGAGTCTGGCTCTATTTCTCATGCA
TTTTTAGATGTGTTTGCTCATGAACCACTGTCTCAAGAATGTCCTTACTGGAATCACCCAAATATCACGATTACACCACA
CATTGCGGCACTCAGCTTTCCTGAGCAAGTGTTTGAGCAGTTCTATGACAACTACTTACGTTGGCGAGATGGGTTCTCTT
TAATTAACTCTATAGATTTTACTAAGGGCTATTAG

Upstream 100 bases:

>100_bases
AAACGAACTTGGATCGGGTTGCGTAACATAGACGATTCTGCACTATTCAAAAAGGACGCTTTAAGTGTACTTGATAACCG
CGAATGACAAAAGGAAGGTT

Downstream 100 bases:

>100_bases
AATATTTAGCCTGTTGTTTCTCGTTTAATTAACAGGCTAAGTATATTAATCCTTAGGTGCAGCCGCTAATTTAGCTCGAA
TAAACTCTAAGTGATCAAGA

Product: 2-ketoacid reductase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNKLLIISKEKEKYLSLLEQADLPDLEICHDIRKAEILLADPPLVIDLLDQFKNLKWLQSTFAGVDSLIQPNLRTDYHLT
NVRGIFGQPISEYVLGYSIQYFRHFKQYAEQQKKKQWQPHTYSSLVNKCMVILGTGSIGSELAATVKQLGFIVYGINRSG
IPPKDSPFDCIFHISEIENAFQYADVIVSTLPKTELTMDLLNSQTLKKCNGALLFNVGRGEVLENYGLLNALESGSISHA
FLDVFAHEPLSQECPYWNHPNITITPHIAALSFPEQVFEQFYDNYLRWRDGFSLINSIDFTKGY

Sequences:

>Translated_304_residues
MNKLLIISKEKEKYLSLLEQADLPDLEICHDIRKAEILLADPPLVIDLLDQFKNLKWLQSTFAGVDSLIQPNLRTDYHLT
NVRGIFGQPISEYVLGYSIQYFRHFKQYAEQQKKKQWQPHTYSSLVNKCMVILGTGSIGSELAATVKQLGFIVYGINRSG
IPPKDSPFDCIFHISEIENAFQYADVIVSTLPKTELTMDLLNSQTLKKCNGALLFNVGRGEVLENYGLLNALESGSISHA
FLDVFAHEPLSQECPYWNHPNITITPHIAALSFPEQVFEQFYDNYLRWRDGFSLINSIDFTKGY
>Mature_304_residues
MNKLLIISKEKEKYLSLLEQADLPDLEICHDIRKAEILLADPPLVIDLLDQFKNLKWLQSTFAGVDSLIQPNLRTDYHLT
NVRGIFGQPISEYVLGYSIQYFRHFKQYAEQQKKKQWQPHTYSSLVNKCMVILGTGSIGSELAATVKQLGFIVYGINRSG
IPPKDSPFDCIFHISEIENAFQYADVIVSTLPKTELTMDLLNSQTLKKCNGALLFNVGRGEVLENYGLLNALESGSISHA
FLDVFAHEPLSQECPYWNHPNITITPHIAALSFPEQVFEQFYDNYLRWRDGFSLINSIDFTKGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=309, Percent_Identity=23.6245954692557, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI87081824, Length=261, Percent_Identity=31.8007662835249, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87082289, Length=197, Percent_Identity=27.9187817258883, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789279, Length=222, Percent_Identity=23.4234234234234, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=246, Percent_Identity=26.4227642276423, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=163, Percent_Identity=32.5153374233129, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=159, Percent_Identity=35.2201257861635, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921140, Length=295, Percent_Identity=26.1016949152542, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34659; Mature: 34659

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLLIISKEKEKYLSLLEQADLPDLEICHDIRKAEILLADPPLVIDLLDQFKNLKWLQS
CCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHH
TFAGVDSLIQPNLRTDYHLTNVRGIFGQPISEYVLGYSIQYFRHFKQYAEQQKKKQWQPH
HHHHHHHHHCCCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TYSSLVNKCMVILGTGSIGSELAATVKQLGFIVYGINRSGIPPKDSPFDCIFHISEIENA
HHHHHHHHHEEEEECCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHEEHHHHHHH
FQYADVIVSTLPKTELTMDLLNSQTLKKCNGALLFNVGRGEVLENYGLLNALESGSISHA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHHCCCCHHHH
FLDVFAHEPLSQECPYWNHPNITITPHIAALSFPEQVFEQFYDNYLRWRDGFSLINSIDF
HHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKGY
CCCC
>Mature Secondary Structure
MNKLLIISKEKEKYLSLLEQADLPDLEICHDIRKAEILLADPPLVIDLLDQFKNLKWLQS
CCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHH
TFAGVDSLIQPNLRTDYHLTNVRGIFGQPISEYVLGYSIQYFRHFKQYAEQQKKKQWQPH
HHHHHHHHHCCCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TYSSLVNKCMVILGTGSIGSELAATVKQLGFIVYGINRSGIPPKDSPFDCIFHISEIENA
HHHHHHHHHEEEEECCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHEEHHHHHHH
FQYADVIVSTLPKTELTMDLLNSQTLKKCNGALLFNVGRGEVLENYGLLNALESGSISHA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHHCCCCHHHH
FLDVFAHEPLSQECPYWNHPNITITPHIAALSFPEQVFEQFYDNYLRWRDGFSLINSIDF
HHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKGY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA