Click here to switch to the map view.

The map label for this gene is gapA2 [H]

Identifier: 59712967

GI number: 59712967

Start: 2648525

End: 2649526

Strand: Reverse

Name: gapA2 [H]

Synonym: VF_2360

Alternate gene names: 59712967

Gene position: 2649526-2648525 (Counterclockwise)

Preceding gene: 59712968

Following gene: 59712966

Centisome position: 91.44

GC content: 41.12

Gene sequence:

>1002_bases
ATGAAAATTAAAGTAGGTATTAACGGATTTGGTCGTATTGGACGTTTGGCGCTAAGAGCCGCATTTGATTGGCCTGAACT
TGAATTTGTACAAATCAATGATGCTGCGGGTGATGCACATACTTTAGCGCACTTATTGGAGTTTGATTCTGTTCAGGGGC
GTTGGCACCATGAAGTGATAAGCAATGGAAGCGACATGATCATTGCAGGTCAAACTCTTAAAACAACGCAAGAAAAAGAC
ATCGATGCGGTTGATTGGTCTGGCTGTGATGTTGTGATTGAAGCGACGGGGGTACACCGCAAGCGTGTATTTTTAGATAA
ATATTTAGCTCAAGGTGTTAAGCGTGTAGTTGTTTCAGCTCCAGTAAAAGAAGAAGGGGTAGTAAACATTGTCGTTGGTG
TGAATGATGATATTTTTGATCCTGCTGTTCACCAAATTGTCACTGCGGCATCTTGTACAACAAACTGTCTCGCTCCAATT
GTTAAAGTTATCAATAAGGGGTTGGGTATCGAAAACGCAGCATTTACCACTATTCATGATTTGACCAATACACAAACCAT
TCTTGATGCACCTCATAAAGATCTTCGCCGTGCACGTGCGTGTGGCATGAGCTTAATCCCAACCACAACGGGTTCAGCTA
AAGCGATTGTAGAAATTTTCCCTGAGCTAAAAGATAAAATCGATGGTCATGCTGTTCGAGTTCCTTTAGCGAATGCATCA
CTCACCGACATTATCTTTGAAGTAAAAAGAGATACCACAGTAGAAGAAGTAAATCAGTTATTAAAGGATGCGGCACAGGG
TGATCTAAAAGGTATTTTAGGTTACGAAGAGCGTCCATTAGTGTCTATTGATTATAAGGGTGACCAACGTTCAACGATTG
TTGATGCGCCATCTACTATGCTTGTAGGTAAACGTATGGTGAAAATTTACGCTTGGTATGACAATGAAATGGGGTATGCA
ACGCGTACTGCTGAATTAGTTCGTAATGTTGGTTTAGCTTAA

Upstream 100 bases:

>100_bases
TATAGAGGGTTTGAAGCAATCGAATTGTTTGCAGGCTCAATATCAAAAAGACACTGATAAATTAAACGCAATGAAAGCGT
GCTGTTAAAGGATAATTAAG

Downstream 100 bases:

>100_bases
TAGTAATCATTGGTTTAAGGAATTTTAAATGACACATCCTACTTGGGAATTACCCGTATCTGACAATGGTAGCGCATTGG
TTTTAACTCCGTGCCCAGGA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKIKVGINGFGRIGRLALRAAFDWPELEFVQINDAAGDAHTLAHLLEFDSVQGRWHHEVISNGSDMIIAGQTLKTTQEKD
IDAVDWSGCDVVIEATGVHRKRVFLDKYLAQGVKRVVVSAPVKEEGVVNIVVGVNDDIFDPAVHQIVTAASCTTNCLAPI
VKVINKGLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELKDKIDGHAVRVPLANAS
LTDIIFEVKRDTTVEEVNQLLKDAAQGDLKGILGYEERPLVSIDYKGDQRSTIVDAPSTMLVGKRMVKIYAWYDNEMGYA
TRTAELVRNVGLA

Sequences:

>Translated_333_residues
MKIKVGINGFGRIGRLALRAAFDWPELEFVQINDAAGDAHTLAHLLEFDSVQGRWHHEVISNGSDMIIAGQTLKTTQEKD
IDAVDWSGCDVVIEATGVHRKRVFLDKYLAQGVKRVVVSAPVKEEGVVNIVVGVNDDIFDPAVHQIVTAASCTTNCLAPI
VKVINKGLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELKDKIDGHAVRVPLANAS
LTDIIFEVKRDTTVEEVNQLLKDAAQGDLKGILGYEERPLVSIDYKGDQRSTIVDAPSTMLVGKRMVKIYAWYDNEMGYA
TRTAELVRNVGLA
>Mature_333_residues
MKIKVGINGFGRIGRLALRAAFDWPELEFVQINDAAGDAHTLAHLLEFDSVQGRWHHEVISNGSDMIIAGQTLKTTQEKD
IDAVDWSGCDVVIEATGVHRKRVFLDKYLAQGVKRVVVSAPVKEEGVVNIVVGVNDDIFDPAVHQIVTAASCTTNCLAPI
VKVINKGLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELKDKIDGHAVRVPLANAS
LTDIIFEVKRDTTVEEVNQLLKDAAQGDLKGILGYEERPLVSIDYKGDQRSTIVDAPSTMLVGKRMVKIYAWYDNEMGYA
TRTAELVRNVGLA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=329, Percent_Identity=38.9057750759878, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI7657116, Length=330, Percent_Identity=36.969696969697, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1788079, Length=333, Percent_Identity=40.5405405405405, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=40.0611620795107, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=38.2789317507418, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=38.2789317507418, Blast_Score=243, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17534677, Length=334, Percent_Identity=38.0239520958084, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17534679, Length=334, Percent_Identity=37.4251497005988, Blast_Score=237, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321631, Length=334, Percent_Identity=41.3173652694611, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=40.4191616766467, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=39.5209580838323, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=38.719512195122, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=38.719512195122, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=38.4146341463415, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=38.4146341463415, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=35.8895705521472, Blast_Score=234, Evalue=4e-62,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36381; Mature: 36381

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVGINGFGRIGRLALRAAFDWPELEFVQINDAAGDAHTLAHLLEFDSVQGRWHHEVI
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHH
SNGSDMIIAGQTLKTTQEKDIDAVDWSGCDVVIEATGVHRKRVFLDKYLAQGVKRVVVSA
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PVKEEGVVNIVVGVNDDIFDPAVHQIVTAASCTTNCLAPIVKVINKGLGIENAAFTTIHD
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELKDKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
LTDIIFEVKRDTTVEEVNQLLKDAAQGDLKGILGYEERPLVSIDYKGDQRSTIVDAPSTM
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCCHH
LVGKRMVKIYAWYDNEMGYATRTAELVRNVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKVGINGFGRIGRLALRAAFDWPELEFVQINDAAGDAHTLAHLLEFDSVQGRWHHEVI
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHH
SNGSDMIIAGQTLKTTQEKDIDAVDWSGCDVVIEATGVHRKRVFLDKYLAQGVKRVVVSA
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PVKEEGVVNIVVGVNDDIFDPAVHQIVTAASCTTNCLAPIVKVINKGLGIENAAFTTIHD
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELKDKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
LTDIIFEVKRDTTVEEVNQLLKDAAQGDLKGILGYEERPLVSIDYKGDQRSTIVDAPSTM
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCCHH
LVGKRMVKIYAWYDNEMGYATRTAELVRNVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]