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The map label for this gene is metF [H]

Identifier: 59712916

GI number: 59712916

Start: 2596201

End: 2597088

Strand: Reverse

Name: metF [H]

Synonym: VF_2309

Alternate gene names: 59712916

Gene position: 2597088-2596201 (Counterclockwise)

Preceding gene: 59712917

Following gene: 59712913

Centisome position: 89.63

GC content: 41.1

Gene sequence:

>888_bases
ATGGCATATTCACACGCAAGCCACTTAGAGTCACTGAATCAAAATATTGCTGAATTAGACGGCAATATCAACGTATCTTT
TGAGTTTTTTCCGCCAAGTTCAGAGAAGATGGAAGAAACACTATGGAACTCTATTCACCGTTTGAAAACACTAAAACCTA
AATTTGTATCGGTAACTTATGGTGCAAACTCAGGTGAACGTGATCGTACTCACTCAATTATTAAAGAAATTAAAGAGCAG
ACCGGTCTTATTGCCGCGCCTCATCTAACGTGTATTGATGCGAGCCGCTCAGAGCTAATTGATATTGCTAACGATTATTG
GGCAAATGGTATTAAAGACATTGTGGCTTTACGTGGTGATATTCCACCGGGTGGTGGGGCTCCTGAAATGTATGCTTCAG
ATTTAGTAGAGCTACTTAAGTCTCAACATGATTTTGATATTTCTGTTGCAGCTTTCCCAGAAGTACACCCAGAAGCGAAA
AGTGCTCAAGCTGATTTACTGAATTTAAAACGCAAAGTAGATGCTGGTGCAAGCCGTGCGATTACTCAGTTCTTTTTTGA
TGTAGAGAGCTATTTACGTTTTCGTGATCGTTGCGTATCTGCTGGTATTGATGTGGAAATCATTCCAGGGATTTTACCAG
TATCGAACTTTAAGCAAGCTTCCCGTTTTGCCAAAATGAACAATGTGAAGATTCCTGGTTGGATGGCACAACAGTTTGAA
GGGCTAGATGATGATCCTGTGACTCGTCAAATGGTTGGAGCAAGTCATGCGATTGATATGACTCGTATTTTGAGCCGTGA
AGGTGTGAAAGATTTCCATTTCTATACGCTTAACCGTGCCGAGCATACTTACGCTCTCTGTCATACATTAGGCGTTCGTC
CAAAATAG

Upstream 100 bases:

>100_bases
TGCATACATTCTGTAGACATATAGACGTCTAAATGATGTCATCATGGATGATGGAATTTTGTAGTATCCCCTTTTGATAC
TGCGGCAGGGAGAGCAAGAC

Downstream 100 bases:

>100_bases
TTTCTAAAATTAATCATATCAAAGTCGCTCATGGTAGCGGCTTTTTTTGTTTTTGATGTCGATATCTTGAGATACAAAGA
GAAAGTAAAAATAAAAAAGG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAYSHASHLESLNQNIAELDGNINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQ
TGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPPGGGAPEMYASDLVELLKSQHDFDISVAAFPEVHPEAK
SAQADLLNLKRKVDAGASRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVKIPGWMAQQFE
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPK

Sequences:

>Translated_295_residues
MAYSHASHLESLNQNIAELDGNINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQ
TGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPPGGGAPEMYASDLVELLKSQHDFDISVAAFPEVHPEAK
SAQADLLNLKRKVDAGASRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVKIPGWMAQQFE
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPK
>Mature_294_residues
AYSHASHLESLNQNIAELDGNINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQT
GLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPPGGGAPEMYASDLVELLKSQHDFDISVAAFPEVHPEAKS
AQADLLNLKRKVDAGASRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVKIPGWMAQQFEG
LDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=33.0985915492958, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=78.9115646258503, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25147441, Length=298, Percent_Identity=30.8724832214765, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147436, Length=298, Percent_Identity=30.8724832214765, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=279, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=299, Percent_Identity=29.4314381270903, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24662658, Length=225, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32918; Mature: 32786

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSHASHLESLNQNIAELDGNINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTY
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
GANSGERDRTHSIIKEIKEQTGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
IPPGGGAPEMYASDLVELLKSQHDFDISVAAFPEVHPEAKSAQADLLNLKRKVDAGASRA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVKIPGWMAQQFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPK
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AYSHASHLESLNQNIAELDGNINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTY
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
GANSGERDRTHSIIKEIKEQTGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
IPPGGGAPEMYASDLVELLKSQHDFDISVAAFPEVHPEAKSAQADLLNLKRKVDAGASRA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVKIPGWMAQQFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPK
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]