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The map label for this gene is lpdA [H]

Identifier: 59712908

GI number: 59712908

Start: 2584670

End: 2586136

Strand: Direct

Name: lpdA [H]

Synonym: VF_2301

Alternate gene names: 59712908

Gene position: 2584670-2586136 (Clockwise)

Preceding gene: 59712907

Following gene: 59712909

Centisome position: 89.2

GC content: 42.54

Gene sequence:

>1467_bases
ATGAAACAAGTCAATGTTGATGTAGCTGTTATCGGTGGTGGTACAGCAGGTCTAGGCTCTTACCGTGCAGCAAAAGCACA
TACTGACAGTGTTGTCATGATTGAAGGCGGCCCTTACGGTACCACTTGTGCTCGCGTTGGTTGTATGCCATCTAAGCTAT
TAATTGCTGCTGCAGAAAGCGTTCACCAAATTGAAAAAGCACCTAAATTTGGTATCCACCCTCAAGGTGAAATCGTAATT
AATGGTCGTGAAGTAATGGAACGCGTTAAGTTTGAGCGTGACCGTTTTGTTGGGTTTGTTTTAGAAGGCGTTGATGAAAT
TCCGGCTGAAGATAAAATCTCTGGATACGCAAAATTTTTAGATGACAACACGCTTCAAGTTGATGATCACACAATCATTA
ATGCAAAACGTATTGTTATCGCTACTGGTTCTCGTCCTGCTTACCCAGCGGTTTGGAATGAACTGGGTGATCGTTTAATC
ATTAATGATGACGTATTTAACTGGGATGACCTACCAAAATCAGTTGCCGTATTTGGCCCTGGTGTTATTGGTCTTGAACT
TGGTCAAGCTCTTCACCGTTTAGGTGTTGAAACCAAACTGTTTGGTTTAGGTGGTCAAGTAGGTCCTGTTACAGACCCAG
AAGTAATGGCGTATGCAAACAAAGCGTTCAATGAAGAGTTTTATCTTGATGCAGACGTAAAAGTTGAAAGCATGAAGCGT
ATTACAACTGAATCAGGTGAAGAGCGCGTTGAAATTCAATTCATCAACAAACAAGGTGAGCTAGAAACTAATATTGTTGA
GTATGTATTAGCAGCAACAGGACGTCGTCCAAATACCGATAAACTGGGCTTAGAAAATACATCAATTGAATTAGATGAAC
GTGGTGTACCAACAGCAGATTTCTATACCCTACAAACATCACTACCAACGGTATTTATTGCTGGTGATGCAAGTAACCAA
TTGCCGTTATTACACGAAGCTGCAGACCAAGCTCGTATTGCTGGTGATAATGCAGGTCGCTTCCCTGAGATCCGTGCAGG
CCTTCGTCGCTCAAAAATCTCAGCGGTATTCTCTGACCCACAAATTGCAATGGTTGGTGAAACTTATAAAGAAATCACAA
CACGCTTAGGTACTTGTGGTTGTTTCGCGACGGGTGAAGTGTCTTTTGAAAACCAAGGCCGCTCACGAGTAATGCTACGT
AATAAAGGCATTCTTCATGTATACGGTGAGCAAGGTACAGGTCGCTTCCTTGGTGCTGAAATGATGGGACCAAATGCAGA
ACATTTAGCTCACTTGTTAGCTTGGGCACACCAAAACAAGATGACAGTGTCAGAAATGCTAGATATGCCTTTCTATCACC
CAGTAATCGAAGAAGGGGTTCGTACTGCTCTTCGTGATTTAAATGCGAAATTACATTTAGGCCCTGAAATGATCAAACAC
TGCATGGATTGTGGTCCGGGCTGTTAA

Upstream 100 bases:

>100_bases
GCTTAATCGTTAACTTAACCGTTAATGAAAAGTAGCCCACAAACCGTGGGCTACCATTTCAAACTCTTCTTCTAAAATAA
ATCTACGTGAGATATGAATT

Downstream 100 bases:

>100_bases
TCAGATTAAAAAGTCCAAAAATAAAAAGACCAATGTTTGCGCATTGGTCTTTTTGCATTTAAAGTTCTCTCCACTCGCTT
ACCAATCAAAGAACTCAATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPKFGIHPQGEIVI
NGREVMERVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLDDNTLQVDDHTIINAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFNWDDLPKSVAVFGPGVIGLELGQALHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKVESMKR
ITTESGEERVEIQFINKQGELETNIVEYVLAATGRRPNTDKLGLENTSIELDERGVPTADFYTLQTSLPTVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMIKH
CMDCGPGC

Sequences:

>Translated_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPKFGIHPQGEIVI
NGREVMERVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLDDNTLQVDDHTIINAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFNWDDLPKSVAVFGPGVIGLELGQALHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKVESMKR
ITTESGEERVEIQFINKQGELETNIVEYVLAATGRRPNTDKLGLENTSIELDERGVPTADFYTLQTSLPTVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMIKH
CMDCGPGC
>Mature_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPKFGIHPQGEIVI
NGREVMERVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLDDNTLQVDDHTIINAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFNWDDLPKSVAVFGPGVIGLELGQALHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKVESMKR
ITTESGEERVEIQFINKQGELETNIVEYVLAATGRRPNTDKLGLENTSIELDERGVPTADFYTLQTSLPTVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMIKH
CMDCGPGC

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=27.1008403361345, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI22035672, Length=325, Percent_Identity=24, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=22.3194748358862, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=22.3194748358862, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=22.3194748358862, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI148277071, Length=308, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI148277065, Length=308, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=24.7899159663866, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87082354, Length=486, Percent_Identity=24.8971193415638, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789915, Length=363, Percent_Identity=27.5482093663912, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=22.9437229437229, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=476, Percent_Identity=27.7310924369748, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983419, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=24.9484536082474, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982272, Length=321, Percent_Identity=25.5451713395639, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=491, Percent_Identity=26.8839103869654, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6325166, Length=391, Percent_Identity=26.3427109974425, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325240, Length=371, Percent_Identity=25.0673854447439, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=27.5210084033613, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640553, Length=496, Percent_Identity=23.1854838709677, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640549, Length=496, Percent_Identity=23.1854838709677, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640551, Length=496, Percent_Identity=23.1854838709677, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17737741, Length=506, Percent_Identity=22.7272727272727, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53270; Mature: 53270

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPKFGIHPQGEIVINGREVMERVKFERDRFVGFVLEGVDEIPAEDKISGYAKFL
HHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHH
DDNTLQVDDHTIINAKRIVIATGSRPAYPAVWNELGDRLIINDDVFNWDDLPKSVAVFGP
CCCEEEECCCEEEECEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEHCCC
GVIGLELGQALHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKVESMKR
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
ITTESGEERVEIQFINKQGELETNIVEYVLAATGRRPNTDKLGLENTSIELDERGVPTAD
HHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
FYTLQTSLPTVFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
EEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECHHHHHHHHHHCCCEEEEECEEEECCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMIKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHH
CMDCGPGC
HHHCCCCC
>Mature Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPKFGIHPQGEIVINGREVMERVKFERDRFVGFVLEGVDEIPAEDKISGYAKFL
HHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHH
DDNTLQVDDHTIINAKRIVIATGSRPAYPAVWNELGDRLIINDDVFNWDDLPKSVAVFGP
CCCEEEECCCEEEECEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEHCCC
GVIGLELGQALHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKVESMKR
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
ITTESGEERVEIQFINKQGELETNIVEYVLAATGRRPNTDKLGLENTSIELDERGVPTAD
HHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
FYTLQTSLPTVFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
EEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECHHHHHHHHHHCCCEEEEECEEEECCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMIKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHH
CMDCGPGC
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]