Click here to switch to the map view.

The map label for this gene is trpS

Identifier: 59712893

GI number: 59712893

Start: 2568668

End: 2569684

Strand: Reverse

Name: trpS

Synonym: VF_2286

Alternate gene names: 59712893

Gene position: 2569684-2568668 (Counterclockwise)

Preceding gene: 59712894

Following gene: 59712892

Centisome position: 88.69

GC content: 42.87

Gene sequence:

>1017_bases
ATGAGCAAGCCCATCGTATTAAGTGGCGTTCAACCATCAGGTGAACTAAGTATCGGTAACTACTTGGGTGCTCTACGTCA
ATGGCAACAGATGCAAGACGATTATGATTGTCAGTACTGTGTGGTAGATTTGCATGCAATTACGGTTCGTCAAGATCCTA
AAGCACTGCATGAAGCGACACTAGATGCGTTGGCAATTTGTCTAGCAGTTGGTGTTGATCCAAAGAAGAGCACGCTATTT
GTTCAGTCACACGTTCCAGAGCATGCTCAGCTTGGTTGGCTTCTTAACTGTTACACTCAAATGGGTGAGTTGAGCCGTAT
GACTCAATTTAAAGATAAATCAGCACGTCATTCAAATGATGTAAATGTTGGTTTATTTGATTACCCGGTGCTAATGGCAG
CGGATATTCTACTTTATGGTGCACATCAAGTGCCTGTTGGTAGTGATCAGAAACAACACTTAGAATTAGCGCGTGATATC
GCAACACGCTTTAATAATATTTATAGCCCAGAAGCGCCAATCTTTACTGTGCCAGAACCTTACATTCCACAAGTGAATGC
TCGTGTTATGAGCCTACAAGATGCGACGAAGAAAATGTCTAAATCAGATGATAACCGTAAGAACGTAATCACGCTGTTAG
AAGAGCCTAAGTCAATTCTTAAGAAGATCAATAAAGCACAAACGGATGCAGAAATGCCACCACGCATTGCTCACGATTGG
GAAAATAAGGCGGGTATTTCAAACCTTATGGGTCTGTACTCTGCTGCGACTGGCAAAACGTTTGAAGAGATCGAAGCACA
ATACCAAGGTGTTGAAATGTACGGTCCTTTCAAGAAAGATGTGGGTGAAGCGATTGTTACTATGCTTGAGCCGATCCAAG
AAGAATACAAGCGTATTCGTGAAGATCGTGCTTATATGGATGCAGTAATGAAAGCGGGTGCTGAGAAAGCGTCTGAGCGT
GCAGCTGTGACATTGAAAAAAGCGTATGAAGCAGTAGGTTTTGTTACTCGCCCATAG

Upstream 100 bases:

>100_bases
TAACTATTAATGTTCAGGCTTATCAAACTATATAGAATAACCATTAAATGATCCCACAGTGGATCATTTAATTTTTACAG
TTAAACAAGGAATCATAACC

Downstream 100 bases:

>100_bases
CGCCTTATTGTATTTAAGTTCCATCAATTGCTCGTAGGCTGACCTGCGAGCAATTTTTATTTTATCCATCTTATAAAGTA
GTTCTTTTATGCTATTAATG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSKPIVLSGVQPSGELSIGNYLGALRQWQQMQDDYDCQYCVVDLHAITVRQDPKALHEATLDALAICLAVGVDPKKSTLF
VQSHVPEHAQLGWLLNCYTQMGELSRMTQFKDKSARHSNDVNVGLFDYPVLMAADILLYGAHQVPVGSDQKQHLELARDI
ATRFNNIYSPEAPIFTVPEPYIPQVNARVMSLQDATKKMSKSDDNRKNVITLLEEPKSILKKINKAQTDAEMPPRIAHDW
ENKAGISNLMGLYSAATGKTFEEIEAQYQGVEMYGPFKKDVGEAIVTMLEPIQEEYKRIREDRAYMDAVMKAGAEKASER
AAVTLKKAYEAVGFVTRP

Sequences:

>Translated_338_residues
MSKPIVLSGVQPSGELSIGNYLGALRQWQQMQDDYDCQYCVVDLHAITVRQDPKALHEATLDALAICLAVGVDPKKSTLF
VQSHVPEHAQLGWLLNCYTQMGELSRMTQFKDKSARHSNDVNVGLFDYPVLMAADILLYGAHQVPVGSDQKQHLELARDI
ATRFNNIYSPEAPIFTVPEPYIPQVNARVMSLQDATKKMSKSDDNRKNVITLLEEPKSILKKINKAQTDAEMPPRIAHDW
ENKAGISNLMGLYSAATGKTFEEIEAQYQGVEMYGPFKKDVGEAIVTMLEPIQEEYKRIREDRAYMDAVMKAGAEKASER
AAVTLKKAYEAVGFVTRP
>Mature_337_residues
SKPIVLSGVQPSGELSIGNYLGALRQWQQMQDDYDCQYCVVDLHAITVRQDPKALHEATLDALAICLAVGVDPKKSTLFV
QSHVPEHAQLGWLLNCYTQMGELSRMTQFKDKSARHSNDVNVGLFDYPVLMAADILLYGAHQVPVGSDQKQHLELARDIA
TRFNNIYSPEAPIFTVPEPYIPQVNARVMSLQDATKKMSKSDDNRKNVITLLEEPKSILKKINKAQTDAEMPPRIAHDWE
NKAGISNLMGLYSAATGKTFEEIEAQYQGVEMYGPFKKDVGEAIVTMLEPIQEEYKRIREDRAYMDAVMKAGAEKASERA
AVTLKKAYEAVGFVTRP

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=335, Percent_Identity=40.2985074626866, Blast_Score=264, Evalue=7e-71,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=50.8379888268156, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1789786, Length=338, Percent_Identity=65.0887573964497, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71982800, Length=338, Percent_Identity=34.9112426035503, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71982793, Length=338, Percent_Identity=34.9112426035503, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6320474, Length=351, Percent_Identity=39.8860398860399, Blast_Score=232, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24666151, Length=333, Percent_Identity=41.7417417417417, Blast_Score=245, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_VIBF1 (Q5E2G5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205669.1
- ProteinModelPortal:   Q5E2G5
- SMR:   Q5E2G5
- STRING:   Q5E2G5
- GeneID:   3279549
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2286
- NMPDR:   fig|312309.3.peg.2286
- eggNOG:   COG0180
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- PhylomeDB:   Q5E2G5
- ProtClustDB:   PRK00927
- BioCyc:   VFIS312309:VF2286-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37795; Mature: 37663

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIVLSGVQPSGELSIGNYLGALRQWQQMQDDYDCQYCVVDLHAITVRQDPKALHEAT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHH
LDALAICLAVGVDPKKSTLFVQSHVPEHAQLGWLLNCYTQMGELSRMTQFKDKSARHSND
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNVGLFDYPVLMAADILLYGAHQVPVGSDQKQHLELARDIATRFNNIYSPEAPIFTVPEP
CEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
YIPQVNARVMSLQDATKKMSKSDDNRKNVITLLEEPKSILKKINKAQTDAEMPPRIAHDW
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
ENKAGISNLMGLYSAATGKTFEEIEAQYQGVEMYGPFKKDVGEAIVTMLEPIQEEYKRIR
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDRAYMDAVMKAGAEKASERAAVTLKKAYEAVGFVTRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKPIVLSGVQPSGELSIGNYLGALRQWQQMQDDYDCQYCVVDLHAITVRQDPKALHEAT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHH
LDALAICLAVGVDPKKSTLFVQSHVPEHAQLGWLLNCYTQMGELSRMTQFKDKSARHSND
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNVGLFDYPVLMAADILLYGAHQVPVGSDQKQHLELARDIATRFNNIYSPEAPIFTVPEP
CEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
YIPQVNARVMSLQDATKKMSKSDDNRKNVITLLEEPKSILKKINKAQTDAEMPPRIAHDW
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
ENKAGISNLMGLYSAATGKTFEEIEAQYQGVEMYGPFKKDVGEAIVTMLEPIQEEYKRIR
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDRAYMDAVMKAGAEKASERAAVTLKKAYEAVGFVTRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA