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The map label for this gene is priA [H]

Identifier: 59712881

GI number: 59712881

Start: 2555471

End: 2557684

Strand: Direct

Name: priA [H]

Synonym: VF_2274

Alternate gene names: 59712881

Gene position: 2555471-2557684 (Clockwise)

Preceding gene: 59712878

Following gene: 59712882

Centisome position: 88.19

GC content: 41.01

Gene sequence:

>2214_bases
ATGATGTTAACCATGCCCTATTCAATCGCTCATGTTGCACTGCCTGTTCCACTTGATCGCACCTTTGATTATCTTATTAA
ATCAGAGCAAAAACCTGTGATCGGTGGACGAGTAAAAGTCCCTTTTGGGCGTCAGCGTTTAATTGGTGTGGTAACAAAAC
TCAGCAATGAATCTGAGTTTGCTCGTGAGAAATTAAAAGCCATCGATGTTGTCATTGATGAACAAAGCTTATGGCCTACC
AATTTATACAAATTATTAAATTGGAGCAGTACGTATTATCAGTATCCATTAGGAGACACCTTAGCGAATGCAATGCCTTC
ATGGCTTAGAAAAGGAAGAGAAGCCAATTTTGCTTCGTTAAAAGCTTGGCAAATTACCGAGCTAGGTAAGAATCAAGATT
TAAATAAGCTAACCCGAGCGCCAAAACAAGCCAAAGCACTCAATTTAGTAAGACATCAACAATGCAGCCATGAGCAAATG
CTTGAAGAAGAAGTGACTGCAGCCACACTAAAAACGATTTCAGATAAAGGTTGGATTAAAGAGGTATCTCTCGCTCCGAC
ACCAAGCTATTGGTATCGAGATGTTGAAGTCACTCAAGAAAAACCTCAACTCAATGAAGAGCAATCTATCGCTATTGCTA
CGGTCAATGCGAATCCTGAATTTGGTTGTTATCTATTGGAAGGGGTTACGGGCTCAGGAAAGACTGAAGTCTATTTACAA
CTTCTAGAACCAATTTTAAAAGCGGGACGACAAGCGCTTATCTTGGTTCCTGAAATTGGTTTAACGCCGCAAACCATTAA
TCGTTTTAAGCGTCGCTTTAATGTACCTGTTGATGTTATTCACTCAGGACTTAATGACACTGAGCGTTTAAATGCATGGC
TAGCAGCAAGAGATAATCACGCCGGTATCATCATTGGTACTCGTTCGGCTCTGTTTACGCCTTTTCATGATTTAGGGATC
ATCATTGTTGATGAAGAACATGATGCCTCTTATAAGCAACAAGACAGCATGAGATATCATGCCCGCGATTTAGCCGTGAT
GAGAGCCAACTTAGATAACATCCCTATTATTCTAGGCTCAGCAACGCCAGCGTTAGAAACATTACACAATGCGAATGTTG
GGAAATATCATCATCTAACACTAACCAAACGCGCAGGTACTGCCATTCCGGCTCGTCATGGTGTTCTTGATGTAAAAGGC
TTATATCTTAATAGCGGTCTATCAGCACCATTAATTGCTCAGATGAGAAAACACTTATCTGAAGGCAATCAGGTTATGCT
GTTTTTAAACCGCAGAGGCTTTTCACCAGCACTAATGTGCCATGAATGTGGTTGGATGGCAGAATGTAAACGTTGTGATG
CGTTCTATACTTTCCACCAACACTCTAATGAAATTCGTTGTCACCACTGTGGTTCACAGCAGCCAATTATCCATCAATGT
CAGGGATGCGGATCGACGCAGTTAATTTCTGTCGGTGTAGGTACAGAACAACTAGAAACACAATTAACCAATCTATTTCC
TGAATACAAAACGGTTCGTATTGATAGAGATAGCACCCGTCGTAAAGGTAGCTTAGAAGACTATTTAGAAGCGATTAAGA
ATAATGAATACCAAATTTTAATTGGTACTCAAATGCTAGCAAAAGGCCACCACTTTCCTAACGTGACTTTGGTTGGGTTA
ATCGACGTAGACAGCTCTCTGTTTAGTAGTGATTTTCGAGCGCCTGAAAGATTAGCTCAATTATTTATTCAGGTTGCAGG
ACGTGCAGGGCGTGCAAGTAAACCAGGTGAGGTAATTCTGCAAACTCATCATCCAGAACACTCTTTATTACAAAGTTTAT
TACATCATGGTTATGGGCATTTTGCAGAGCACGCTTTAAAAGAGCGTAAATTTGCTCAATTACCACCTTATACCCATTTG
TCTCTTTTTAGAGCTGAAGCAAATAATAACGAAAACGTGGAACAATTCTTACGACAAGTGCGTGATATTTTAGAAAGCCA
CCCACAATTTCATGATGCAACACCAGTACTTGGGCCGATCCCTGCCCCATTAGCTCGTCGCGCCGGTAAATATCGTTGGC
AGCTATTGTTCCAGGCTCCAAACCGGACAGTAATGCAATCAATGCTTCGCGGTGCAAAAACCGCCATTGAACTTTTACCC
CTAGCAAAAAAAGTTCGATGGACTTTAGATATTGAGCCACAAGATTTAAGTTAA

Upstream 100 bases:

>100_bases
GGAGCGATCAATCAACTGATCTGTTACTTTTTTCACCGATTTTTTTACCCATTTGCGATATTTCTATCTAAGGGTAGACT
ATTCTCATTCCAATTTCTTA

Downstream 100 bases:

>100_bases
TGTCGGTTTTATCAACAATTTTCCTGTTGAAAAAGTGAAACAATCGGCCTTCATGTGAGAGAAATCACAATACAGATGTA
ATTTTTGTTAAACGAGCCGT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MMLTMPYSIAHVALPVPLDRTFDYLIKSEQKPVIGGRVKVPFGRQRLIGVVTKLSNESEFAREKLKAIDVVIDEQSLWPT
NLYKLLNWSSTYYQYPLGDTLANAMPSWLRKGREANFASLKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQM
LEEEVTAATLKTISDKGWIKEVSLAPTPSYWYRDVEVTQEKPQLNEEQSIAIATVNANPEFGCYLLEGVTGSGKTEVYLQ
LLEPILKAGRQALILVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLAARDNHAGIIIGTRSALFTPFHDLGI
IIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIILGSATPALETLHNANVGKYHHLTLTKRAGTAIPARHGVLDVKG
LYLNSGLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAFYTFHQHSNEIRCHHCGSQQPIIHQC
QGCGSTQLISVGVGTEQLETQLTNLFPEYKTVRIDRDSTRRKGSLEDYLEAIKNNEYQILIGTQMLAKGHHFPNVTLVGL
IDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLHHGYGHFAEHALKERKFAQLPPYTHL
SLFRAEANNNENVEQFLRQVRDILESHPQFHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLP
LAKKVRWTLDIEPQDLS

Sequences:

>Translated_737_residues
MMLTMPYSIAHVALPVPLDRTFDYLIKSEQKPVIGGRVKVPFGRQRLIGVVTKLSNESEFAREKLKAIDVVIDEQSLWPT
NLYKLLNWSSTYYQYPLGDTLANAMPSWLRKGREANFASLKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQM
LEEEVTAATLKTISDKGWIKEVSLAPTPSYWYRDVEVTQEKPQLNEEQSIAIATVNANPEFGCYLLEGVTGSGKTEVYLQ
LLEPILKAGRQALILVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLAARDNHAGIIIGTRSALFTPFHDLGI
IIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIILGSATPALETLHNANVGKYHHLTLTKRAGTAIPARHGVLDVKG
LYLNSGLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAFYTFHQHSNEIRCHHCGSQQPIIHQC
QGCGSTQLISVGVGTEQLETQLTNLFPEYKTVRIDRDSTRRKGSLEDYLEAIKNNEYQILIGTQMLAKGHHFPNVTLVGL
IDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLHHGYGHFAEHALKERKFAQLPPYTHL
SLFRAEANNNENVEQFLRQVRDILESHPQFHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLP
LAKKVRWTLDIEPQDLS
>Mature_737_residues
MMLTMPYSIAHVALPVPLDRTFDYLIKSEQKPVIGGRVKVPFGRQRLIGVVTKLSNESEFAREKLKAIDVVIDEQSLWPT
NLYKLLNWSSTYYQYPLGDTLANAMPSWLRKGREANFASLKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQM
LEEEVTAATLKTISDKGWIKEVSLAPTPSYWYRDVEVTQEKPQLNEEQSIAIATVNANPEFGCYLLEGVTGSGKTEVYLQ
LLEPILKAGRQALILVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLAARDNHAGIIIGTRSALFTPFHDLGI
IIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIILGSATPALETLHNANVGKYHHLTLTKRAGTAIPARHGVLDVKG
LYLNSGLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAFYTFHQHSNEIRCHHCGSQQPIIHQC
QGCGSTQLISVGVGTEQLETQLTNLFPEYKTVRIDRDSTRRKGSLEDYLEAIKNNEYQILIGTQMLAKGHHFPNVTLVGL
IDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLHHGYGHFAEHALKERKFAQLPPYTHL
SLFRAEANNNENVEQFLRQVRDILESHPQFHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLP
LAKKVRWTLDIEPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=54.0577716643741, Blast_Score=805, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83108; Mature: 83108

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLTMPYSIAHVALPVPLDRTFDYLIKSEQKPVIGGRVKVPFGRQRLIGVVTKLSNESEF
CEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHH
AREKLKAIDVVIDEQSLWPTNLYKLLNWSSTYYQYPLGDTLANAMPSWLRKGREANFASL
HHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHE
KAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEEEVTAATLKTISDKGWIK
EEEEEHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEE
EVSLAPTPSYWYRDVEVTQEKPQLNEEQSIAIATVNANPEFGCYLLEGVTGSGKTEVYLQ
EEECCCCCCHHCCCCEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHH
LLEPILKAGRQALILVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLAARDNH
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHEEECCCC
AGIIIGTRSALFTPFHDLGIIIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIILGS
CEEEEECCHHHHCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
ATPALETLHNANVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLNSGLSAPLIAQMRKHLS
CCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHC
EGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAFYTFHQHSNEIRCHHCGSQQPIIHQC
CCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEEEECCCCCCHHHHH
QGCGSTQLISVGVGTEQLETQLTNLFPEYKTVRIDRDSTRRKGSLEDYLEAIKNNEYQIL
CCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEE
IGTQMLAKGHHFPNVTLVGLIDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVIL
EEHHHHHCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
QTHHPEHSLLQSLLHHGYGHFAEHALKERKFAQLPPYTHLSLFRAEANNNENVEQFLRQV
EECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCHHHHHHHH
RDILESHPQFHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLP
HHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LAKKVRWTLDIEPQDLS
HHHHEEEEEECCCCCCC
>Mature Secondary Structure
MMLTMPYSIAHVALPVPLDRTFDYLIKSEQKPVIGGRVKVPFGRQRLIGVVTKLSNESEF
CEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHH
AREKLKAIDVVIDEQSLWPTNLYKLLNWSSTYYQYPLGDTLANAMPSWLRKGREANFASL
HHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHE
KAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEEEVTAATLKTISDKGWIK
EEEEEHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEE
EVSLAPTPSYWYRDVEVTQEKPQLNEEQSIAIATVNANPEFGCYLLEGVTGSGKTEVYLQ
EEECCCCCCHHCCCCEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHH
LLEPILKAGRQALILVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLAARDNH
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHEEECCCC
AGIIIGTRSALFTPFHDLGIIIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIILGS
CEEEEECCHHHHCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
ATPALETLHNANVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLNSGLSAPLIAQMRKHLS
CCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHC
EGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAFYTFHQHSNEIRCHHCGSQQPIIHQC
CCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEEEECCCCCCHHHHH
QGCGSTQLISVGVGTEQLETQLTNLFPEYKTVRIDRDSTRRKGSLEDYLEAIKNNEYQIL
CCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEE
IGTQMLAKGHHFPNVTLVGLIDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVIL
EEHHHHHCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
QTHHPEHSLLQSLLHHGYGHFAEHALKERKFAQLPPYTHLSLFRAEANNNENVEQFLRQV
EECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCHHHHHHHH
RDILESHPQFHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLP
HHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LAKKVRWTLDIEPQDLS
HHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]