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The map label for this gene is pykF [H]

Identifier: 59712865

GI number: 59712865

Start: 2533972

End: 2535384

Strand: Direct

Name: pykF [H]

Synonym: VF_2258

Alternate gene names: 59712865

Gene position: 2533972-2535384 (Clockwise)

Preceding gene: 59712864

Following gene: 59712866

Centisome position: 87.45

GC content: 41.47

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTTTGTACGATCGGCCCAAAAACTGAATCAGTTGAAAAACTAACTGAACTAGCAAATGCTGG
TATGAACGTTATGCGCCTAAACTTCTCACACGGCGACTTCGCAGAACACGGCCAACGTATTGATAACGTACGTCAAGTAA
TGAAAAACACTGGTAAACAACTTGCTATTCTTCTTGATACTAAAGGTCCTGAAATCCGTACAATCAAACTAGAAAACGGC
GACGATGTTGCTCTAGTTGCAGGTCAAGATTTCACATTCACAACAGATACTTCAGTTGTTGGTAATAAAGACGTTGTTGC
TGTTACTTACCCAGGTTTTGCAAACGACCTTGAAGTTGGTAACACTATCCTTGTTGATGACGGCCTAATCGAAATGGAAG
TTGTTGCGACAACTGAAACTGAAGTTAAGTGTAAAGTTCTTAACAACGGTGACCTAGGCGAAAACAAAGGTGTTAACCTT
CCTGGCGTTTCTGTTCAACTTCCAGCTCTATCTGAAAAAGATAAAGCTGACCTTAAATTTGGTTGTGAGCAAGGCGTTGA
TTTCGTTGCTGCTTCTTTCATTCGTAAAGAAGAAGACGTTAAAGAAATCCGTGAACTACTTGTTGCTAACGGCGGCGAGA
ACATTCAAATCATCTCTAAGATTGAAAACCAAGAAGGTGTTGATAACTTCGATTCAATCCTAGAAGCTTCTGACGGTATC
ATGGTTGCTCGTGGTGACCTTGGTGTTGAAATCCCAGTTGAAGAAGTAATCTTCGCTCAGAAGATGATGATCGAGAAATG
TAACCGTGCACGTAAAGTTGTAATCACTGCAACTCAAATGCTTGATTCTATGATCAAAAACCCACGTCCAACTCGTGCTG
AAGCGGGTGACGTTGCGAACGCAATCATGGATGGTACTGATGCTGTAATGCTTTCAGGTGAATCTGCTAAAGGTAAATAC
CCAGTTGAAGCTGTAACTATTATGGCTCAAATCTGTGCTCGTACTGACGCAGCTCTTAAAGCAGAACTAGGTTCTCGTCT
AGATAGCCCACGTCTACGTATCACTGAAGCGGTATGTAAAGGTGCAGTAGATACAGCTGAGAAACTAAACGCTCCTGTTA
TCGTTGTTGCAACTGAAGCTGGTAAATCAGCTCGTTCAATCCGTAAATACTTCCCAACAGCTAAAATCATCGCTGTAACT
ACTAATACTAAGACTGCTGCACAACTATGTCTTTCTAAAGGTATTACTCCAATGATCGTTGATTCAATCGATAGCACTGA
CGCATTCTACCTACGTGGTAAAGAGTTAGCTCTTGAATCAGGTATTGGCGCTAAAGGCGATATCGTTGTTATGGTTTCTG
GTGCTCTAGTTCCATCTGGTACTACAAACACAGCTTCTGTACACGTTCTATAA

Upstream 100 bases:

>100_bases
TTTGAAGATAAAATTGTTGTAATTTTTCAACACTTCAAACATTAGCTGGGCATTACTGCGTTTATCATTTTAAATATACT
CATCTGGAGAGATTTCTCAC

Downstream 100 bases:

>100_bases
TTCATTGAATTTATAAACAAGTATAACGAGTTAATAAAAAGAGGGCTTTCGCCCTCTTTTTTTATGAAATTTTCTTGCCC
AAAACAAACAATACGCTATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFAEHGQRIDNVRQVMKNTGKQLAILLDTKGPEIRTIKLENG
DDVALVAGQDFTFTTDTSVVGNKDVVAVTYPGFANDLEVGNTILVDDGLIEMEVVATTETEVKCKVLNNGDLGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLVANGGENIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGESAKGKY
PVEAVTIMAQICARTDAALKAELGSRLDSPRLRITEAVCKGAVDTAEKLNAPVIVVATEAGKSARSIRKYFPTAKIIAVT
TNTKTAAQLCLSKGITPMIVDSIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFAEHGQRIDNVRQVMKNTGKQLAILLDTKGPEIRTIKLENG
DDVALVAGQDFTFTTDTSVVGNKDVVAVTYPGFANDLEVGNTILVDDGLIEMEVVATTETEVKCKVLNNGDLGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLVANGGENIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGESAKGKY
PVEAVTIMAQICARTDAALKAELGSRLDSPRLRITEAVCKGAVDTAEKLNAPVIVVATEAGKSARSIRKYFPTAKIIAVT
TNTKTAAQLCLSKGITPMIVDSIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFAEHGQRIDNVRQVMKNTGKQLAILLDTKGPEIRTIKLENG
DDVALVAGQDFTFTTDTSVVGNKDVVAVTYPGFANDLEVGNTILVDDGLIEMEVVATTETEVKCKVLNNGDLGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLVANGGENIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGESAKGKY
PVEAVTIMAQICARTDAALKAELGSRLDSPRLRITEAVCKGAVDTAEKLNAPVIVVATEAGKSARSIRKYFPTAKIIAVT
TNTKTAAQLCLSKGITPMIVDSIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=48.0572597137014, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=48.0572597137014, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=47.9508196721311, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=46.8431771894094, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=46.8431771894094, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=52.0833333333333, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=52.0833333333333, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=47.4576271186441, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=47.4576271186441, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.5116279069767, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=78.0851063829787, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=37.9746835443038, Blast_Score=289, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.1666666666667, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=45.0413223140496, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=45.0413223140496, Blast_Score=345, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=45.0413223140496, Blast_Score=345, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=45.0413223140496, Blast_Score=344, Evalue=6e-95,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=45.1143451143451, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324923, Length=482, Percent_Identity=43.5684647302905, Blast_Score=354, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=46.8302658486708, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=48.024948024948, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24648966, Length=443, Percent_Identity=46.7268623024831, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=34.375, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24646914, Length=301, Percent_Identity=44.5182724252492, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50160; Mature: 50160

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFAEHGQRIDNVRQVMKNTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLENGDDVALVAGQDFTFTTDTSVVGNKDVVAVTYPGFANDLEVG
EEEEEECCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCCCCCC
NTILVDDGLIEMEVVATTETEVKCKVLNNGDLGENKGVNLPGVSVQLPALSEKDKADLKF
CEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRELLVANGGENIQIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGESAKGKYPVEAVTIMAQICARTDAALKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLNAPVIVVATEAGKSARSIRKYFPTAKIIAVTTNTKTAAQLCLSKGITPMIV
CCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DSIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
ECCCCCCEEEEECCEEEHCCCCCCCCCEEEEEECEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFAEHGQRIDNVRQVMKNTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLENGDDVALVAGQDFTFTTDTSVVGNKDVVAVTYPGFANDLEVG
EEEEEECCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCCCCCC
NTILVDDGLIEMEVVATTETEVKCKVLNNGDLGENKGVNLPGVSVQLPALSEKDKADLKF
CEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRELLVANGGENIQIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGESAKGKYPVEAVTIMAQICARTDAALKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLNAPVIVVATEAGKSARSIRKYFPTAKIIAVTTNTKTAAQLCLSKGITPMIV
CCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DSIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
ECCCCCCEEEEECCEEEHCCCCCCCCCEEEEEECEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]