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The map label for this gene is mutT [H]

Identifier: 59712799

GI number: 59712799

Start: 2462523

End: 2462924

Strand: Reverse

Name: mutT [H]

Synonym: VF_2192

Alternate gene names: 59712799

Gene position: 2462924-2462523 (Counterclockwise)

Preceding gene: 59712800

Following gene: 59712790

Centisome position: 85.0

GC content: 35.07

Gene sequence:

>402_bases
ATGAAACGATTACATATAGTTGCTGCAATCATTTTAAATGCAGAAAAGAGCCAAGTCTTTATTACTAAACGTCCAGATAA
AGTACATAAAGGAGGCTTTTGGGAGTTTCCTGGAGGTAAAGTTGAAGCAGGAGAAAGTGCAGAACAAGCTCTGATAAGAG
AGTTGAATGAAGAGATTGGTATTAACTCAACTGAGCTCGATATATTTGAATCTTTATCTCATGACTATCCTGATAAATCG
CTTTATTTTGATTTTTTTACGGTAACTCAGTTTGATAACCAACCTTATGGTAAAGAAGGGCAAGAAGGTTTGTGGGTATC
TATTTCCTCGCTTAAAGAGTATGAGTTTCCTGAAGCGAATGTACCTGTATTAAATAAAGTCGTAGAGCAATTTAGTTCAT
AA

Upstream 100 bases:

>100_bases
AAGATCTAGTTGATACTTATTGTTCTTAATTGAAAAATAAAAGCCGCTATCATTTAGCGGCTTTTTTATAAGCGTGATAT
GGATATAGAAAAGGTTATAC

Downstream 100 bases:

>100_bases
TTCTCTGACTTCATATAATAGGTACAAAAAAAGCGATCATGATGATCGCTTTTTTATTTGGAATCGGTATTAGTATTGAT
CTTCAGACCAACCATCAGAG

Product: nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKRLHIVAAIILNAEKSQVFITKRPDKVHKGGFWEFPGGKVEAGESAEQALIRELNEEIGINSTELDIFESLSHDYPDKS
LYFDFFTVTQFDNQPYGKEGQEGLWVSISSLKEYEFPEANVPVLNKVVEQFSS

Sequences:

>Translated_133_residues
MKRLHIVAAIILNAEKSQVFITKRPDKVHKGGFWEFPGGKVEAGESAEQALIRELNEEIGINSTELDIFESLSHDYPDKS
LYFDFFTVTQFDNQPYGKEGQEGLWVSISSLKEYEFPEANVPVLNKVVEQFSS
>Mature_133_residues
MKRLHIVAAIILNAEKSQVFITKRPDKVHKGGFWEFPGGKVEAGESAEQALIRELNEEIGINSTELDIFESLSHDYPDKS
LYFDFFTVTQFDNQPYGKEGQEGLWVSISSLKEYEFPEANVPVLNKVVEQFSS

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=127, Percent_Identity=44.0944881889764, Blast_Score=120, Evalue=4e-29,
Organism=Escherichia coli, GI1788056, Length=125, Percent_Identity=33.6, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 15115; Mature: 15115

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLHIVAAIILNAEKSQVFITKRPDKVHKGGFWEFPGGKVEAGESAEQALIRELNEEIG
CCHHHEEEHHEECCCCCEEEEEECCCCCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHC
INSTELDIFESLSHDYPDKSLYFDFFTVTQFDNQPYGKEGQEGLWVSISSLKEYEFPEAN
CCCCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCHHHCCCCCCC
VPVLNKVVEQFSS
CCHHHHHHHHHCC
>Mature Secondary Structure
MKRLHIVAAIILNAEKSQVFITKRPDKVHKGGFWEFPGGKVEAGESAEQALIRELNEEIG
CCHHHEEEHHEECCCCCEEEEEECCCCCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHC
INSTELDIFESLSHDYPDKSLYFDFFTVTQFDNQPYGKEGQEGLWVSISSLKEYEFPEAN
CCCCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCHHHCCCCCCC
VPVLNKVVEQFSS
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3033442; 1630901; 9278503; 2841285; 8202364; 3288626; 1851162; 1309939; 7578113; 9063868 [H]