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The map label for this gene is pilB [H]

Identifier: 59712793

GI number: 59712793

Start: 2456927

End: 2458615

Strand: Direct

Name: pilB [H]

Synonym: VF_2186

Alternate gene names: 59712793

Gene position: 2456927-2458615 (Clockwise)

Preceding gene: 59712792

Following gene: 59712794

Centisome position: 84.79

GC content: 41.98

Gene sequence:

>1689_bases
ATGCACACCAACCTCGCCTCTATCTTGCGTCAAGCTGAGTTAATCAGTGCGACGCAAGAAAGCAGTATCATAGAGTCAGT
TCAAGCAGAAACGCACACTGTGCCTTCTGCTCTTTTAGCTCAGGGGATATTTACTCCTGATGAACTCATAAGTCACCTTG
CTCAAATCTTTGGCCTTGATGTGATCAACGTGACTCAATTTGATTATGCCACCACTTGTCAAAAGCTGGGACTGCGCGAT
CTCATTACTCGCTATCAAGCTCTTCCTGTAAGTCACGACAGTTGCACGATTCGTCTTGCTGTAGCTGATCCAAGTATTGC
TCAAATAGAAGATGATTTTCGTTTTACTAGCGGAAAACAAATTGAATTAGTACTTGCAGATCAAGGACAAATCCAAGCAG
CCATTCGACGCGTCTACGGTGCAGGATTATCTGCAAATGACAATCAACATAAAGAGATAACCCAAGATGAGTTAGCTTCA
TTAGTGGATGTCTCACTTGATGAGATAAGAGCTACAGAAGATCTCAGCCAAGACGATGCCCCCGTCACTCGCTTTATTAA
CCAAATTTTATTAGAAGCGGTGCGTAAAGGAGTATCAGATATTCATTTTGAACCCTACGAAGATAATTATCGTGTGCGTT
TTCGCTGCGATGGGATCTTAGTTGAAAACAGCCGTCCATCCGCTCACTTAAGTCGTCGTTTATCTGCTCGCCTTAAAATC
ATGGCAAAATTAGACATTGCAGAACGTCGTCTACCACAAGATGGGCGTATTAAACTCAAATTAAACAGTACCACTTCGAT
TGATATGCGAGTTTCTTCACTGCCAACTTTATGGGGTGAGAAAATCGTACTGCGTTTATTAGACAGCAGTGCTGCTAATT
TAAATATTGATAAACTTGGCTATAGCGATCAACAAAAAGCCCTATATTTAGAAGCCCTAAAACGCCCTCAAGGCATGATA
TTAATGACAGGCCCTACAGGCAGTGGGAAAACCGTCTCTCTTTATTCTGGTCTTAATATTCTCAACACCACGGAACAAAA
CATTTCAACGGCGGAAGATCCAGTTGAGATCAACTTAAATGGGATCAACCAAGTTCAAGTTAACCCTAAAATCGGCCTTA
CTTTCGCTCACGCTTTACGATCTTTTTTACGCCAAGATCCAGATATCGTCATGGTAGGTGAAATACGAGATCTCGAAACC
GCTGAGATTGCTGTCAAAGCAGCTCAAACCGGGCACTTAGTTCTTTCTACCCTACATACAAATTCCGCAGCAGAAACCGT
CGTTCGACTAGGCAACATGGGGATTGAAAACTTTAACTTAGCTTCCTCTCTTAGTTTGATTATTGCGCAACGGCTGGCTC
GTCGTCTCTGCAATTTATGCAAGCAAGAAGATCATTTAAGTGATGAGTTGCGAGAAGAATATAACATTGCACCTTCAGCC
ATTATTTATCAAGCCAATAAAGAAGGTTGCAATGAGTGCACTCATGGTTACTCAGGGCGTATTGGTATTTATGAAGTGAT
GCCGTTCACCCGAGAACTGTCTGTTGCCATTTTAAAAGGTGCAACCGTTAGACAAATAGAACAAATCGCCATTCAACAAG
GTATGGATAACCTAAAAACCTCAGGAATTAAACGATTAGAAGATGGCACCACCAGCTTACAAGAACTAAACCGTGTGCTC
TATTTTTAA

Upstream 100 bases:

>100_bases
CTCAACAGTGACCATATCTCGTCAAGACACTGGAAAATGGGATTGTCAATATTCTGTAAGTGATTTAAATATCCCAGGCT
GTCAAGGCCAATAAATACCC

Downstream 100 bases:

>100_bases
AGATAAGTAGATAATTCAAATGCCTAAAACAAAAAAGAAATCCAAAGCAGTCGTATTAAAAAGCTTTCAATGGCGTGGCA
TTAACAGTTCAGGGAAAAAA

Product: pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLDVINVTQFDYATTCQKLGLRD
LITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQIELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELAS
LVDVSLDEIRATEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYSDQQKALYLEALKRPQGMI
LMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLNGINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKTSGIKRLEDGTTSLQELNRVL
YF

Sequences:

>Translated_562_residues
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLDVINVTQFDYATTCQKLGLRD
LITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQIELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELAS
LVDVSLDEIRATEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYSDQQKALYLEALKRPQGMI
LMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLNGINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKTSGIKRLEDGTTSLQELNRVL
YF
>Mature_562_residues
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLDVINVTQFDYATTCQKLGLRD
LITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQIELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELAS
LVDVSLDEIRATEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYSDQQKALYLEALKRPQGMI
LMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLNGINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKTSGIKRLEDGTTSLQELNRVL
YF

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=418, Percent_Identity=47.3684210526316, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1786296, Length=489, Percent_Identity=40.6952965235174, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=32.6241134751773, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62246; Mature: 62246

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCC
VINVTQFDYATTCQKLGLRDLITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQ
EEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCEECCCCE
IELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELASLVDVSLDEIRATEDLSQDDA
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHHHHCCCCCCC
PVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHH
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLG
HHHCCHHHHHCCCCCEEEEEECCCCEEEEEHHHCCCHHHHHHHHHHHCCCCCCCEEHHCC
YSDQQKALYLEALKRPQGMILMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLN
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEEC
GINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLHT
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEECCCCCEEEEEECC
NSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCE
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKT
EEEECCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHH
SGIKRLEDGTTSLQELNRVLYF
HHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCC
VINVTQFDYATTCQKLGLRDLITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQ
EEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCEECCCCE
IELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELASLVDVSLDEIRATEDLSQDDA
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHHHHCCCCCCC
PVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHH
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLG
HHHCCHHHHHCCCCCEEEEEECCCCEEEEEHHHCCCHHHHHHHHHHHCCCCCCCEEHHCC
YSDQQKALYLEALKRPQGMILMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLN
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEEC
GINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLHT
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEECCCCCEEEEEECC
NSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCE
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKT
EEEECCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHH
SGIKRLEDGTTSLQELNRVLYF
HHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]