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The map label for this gene is panB

Identifier: 59712776

GI number: 59712776

Start: 2438521

End: 2439315

Strand: Direct

Name: panB

Synonym: VF_2169

Alternate gene names: 59712776

Gene position: 2438521-2439315 (Clockwise)

Preceding gene: 59712775

Following gene: 59712777

Centisome position: 84.16

GC content: 42.26

Gene sequence:

>795_bases
ATGAAAAAAATCTCTATTCATGACTTAATGAAGTGGAAGCAAGAAGGCAAAAAATTTGCCACTGTTACAGCATATGATGC
TAGCTTCGCACAACTATTTGAACAGCAAGAAGTCCCTGTTTTATTAGTCGGTGATTCATTAGGTATGGTTTTACAAGGTA
AGAGCGACACTCTACCTGTAACCACTGAAGAGATCGCTTACCATACTCGCTGTGTACGTGCAGGCAGCCCAAATAGCCTA
TTAATGGCAGATATGCCATTCATGAGCTACGCAACTCCTGAGCAAGCGTGTGAGAATGCTGGAAAGCTAATGCAAGCAGG
CGCTAACATGGTGAAGATTGAAGGCGGTGAATGGATAGCTGAAACAGTTCGAGTCCTTGCAGAGCGTGCCGTCCCAGTTT
GTGCGCACCTTGGTTTAACTCCTCAATCAGTTAATATTTTTGGTGGGTTCCGCATTCAAGGTCGTGATGAAGCTAAAGCA
GAGCAAATGGTAAAAGATGCCTTAACGCTTGAAGCTGCTGGTGCTCAAATTATCCTACTAGAATGCGTTCCAGCTTCTCT
TGCTGAGCGTATTACAAAAGCATGTACCGTTCCTGTTATTGGTATTGGTGCAGGTAATGTGACTGATGGTCAAATCCTTG
TAATGCACGATATGTTTGGTATTTCAGCTAACTACATGCCTAAATTCTCAAAAAACTTTTTGGCAGAAACTGGTGATATG
CGTAAAGCGGTCACTAAATACATTCAAGATGTTGAGCAAGGTGTATTCCCTGTTGCTGAACATACATTTAACTAA

Upstream 100 bases:

>100_bases
ACTTTACCTGATGGCACAACCCTTTCTCAGTTACTAAAAACTGTTGATAGAAATGGTTTGTCTATTTGGGAAGATTAATC
GCTTCTAGTAAGGATGTTTT

Downstream 100 bases:

>100_bases
GGAAACGTAATGGACATTTTTTCTGAAATTTTACCATTACGCGAGCAGATCAAAGCTTGGAAACGCGAAGGTAAACGTAT
TGCTTTCGTTCCAACAATGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase 1; KPHMT 1

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKISIHDLMKWKQEGKKFATVTAYDASFAQLFEQQEVPVLLVGDSLGMVLQGKSDTLPVTTEEIAYHTRCVRAGSPNSL
LMADMPFMSYATPEQACENAGKLMQAGANMVKIEGGEWIAETVRVLAERAVPVCAHLGLTPQSVNIFGGFRIQGRDEAKA
EQMVKDALTLEAAGAQIILLECVPASLAERITKACTVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVTKYIQDVEQGVFPVAEHTFN

Sequences:

>Translated_264_residues
MKKISIHDLMKWKQEGKKFATVTAYDASFAQLFEQQEVPVLLVGDSLGMVLQGKSDTLPVTTEEIAYHTRCVRAGSPNSL
LMADMPFMSYATPEQACENAGKLMQAGANMVKIEGGEWIAETVRVLAERAVPVCAHLGLTPQSVNIFGGFRIQGRDEAKA
EQMVKDALTLEAAGAQIILLECVPASLAERITKACTVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVTKYIQDVEQGVFPVAEHTFN
>Mature_264_residues
MKKISIHDLMKWKQEGKKFATVTAYDASFAQLFEQQEVPVLLVGDSLGMVLQGKSDTLPVTTEEIAYHTRCVRAGSPNSL
LMADMPFMSYATPEQACENAGKLMQAGANMVKIEGGEWIAETVRVLAERAVPVCAHLGLTPQSVNIFGGFRIQGRDEAKA
EQMVKDALTLEAAGAQIILLECVPASLAERITKACTVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVTKYIQDVEQGVFPVAEHTFN

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=66.2878787878788, Blast_Score=381, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=34.0659340659341, Blast_Score=158, Evalue=9e-40,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB1_VIBF1 (Q5E2T2)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205552.1
- HSSP:   P31057
- ProteinModelPortal:   Q5E2T2
- SMR:   Q5E2T2
- STRING:   Q5E2T2
- GeneID:   3279367
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2169
- NMPDR:   fig|312309.3.peg.2169
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q5E2T2
- ProtClustDB:   PRK00311
- BioCyc:   VFIS312309:VF2169-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28674; Mature: 28674

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKISIHDLMKWKQEGKKFATVTAYDASFAQLFEQQEVPVLLVGDSLGMVLQGKSDTLPV
CCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCC
TTEEIAYHTRCVRAGSPNSLLMADMPFMSYATPEQACENAGKLMQAGANMVKIEGGEWIA
CHHHHHHHHHHEECCCCCCEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHH
ETVRVLAERAVPVCAHLGLTPQSVNIFGGFRIQGRDEAKAEQMVKDALTLEAAGAQIILL
HHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEE
ECVPASLAERITKACTVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
EECCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RKAVTKYIQDVEQGVFPVAEHTFN
HHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MKKISIHDLMKWKQEGKKFATVTAYDASFAQLFEQQEVPVLLVGDSLGMVLQGKSDTLPV
CCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCC
TTEEIAYHTRCVRAGSPNSLLMADMPFMSYATPEQACENAGKLMQAGANMVKIEGGEWIA
CHHHHHHHHHHEECCCCCCEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHH
ETVRVLAERAVPVCAHLGLTPQSVNIFGGFRIQGRDEAKAEQMVKDALTLEAAGAQIILL
HHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEE
ECVPASLAERITKACTVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
EECCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RKAVTKYIQDVEQGVFPVAEHTFN
HHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA