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The map label for this gene is 59712751

Identifier: 59712751

GI number: 59712751

Start: 2397077

End: 2398795

Strand: Direct

Name: 59712751

Synonym: VF_2144

Alternate gene names: NA

Gene position: 2397077-2398795 (Clockwise)

Preceding gene: 59712750

Following gene: 59712752

Centisome position: 82.73

GC content: 40.37

Gene sequence:

>1719_bases
ATGCAACTGTTAACTAACCATATTGGATATGAAACAAACGGACCGAAATCAGCAGTATTATTGGCTACCACTGCCCTTAC
GCAGCCAATATCAGGTCATATTGTTTGTACTCAAACACAAGAGGTCAAATACCGTTTTTCTATTTCTTCATCAGACCAAG
TCGCTCATTGGCATCAAGGTTATTTTGCTGAGATAACATTTACTGATTTTACTGAAGAAGGTGAATTCATTCTTGTTATC
GAAGGTGCAATGTCTCACTCTTTTGTTATCAAAACCAACTTATTAATGGCGCACACATTTTCTGATGTTATGCACTATTT
TAAATCACAACGATGTGGTGGGATTTTCGATAAACAAGATAAACAAGCTCCTTTATTAAATAGCAACGAAACCGTCGATG
TTCATGGCGGTTGGTATGATGCATCCGGTGATGTGAGTAAATACCTTAGTCACTTATCTTATGCTAATTACCTTAATCCC
CAGCAAATTCCGATGGTTGTTTGGAATATGCTCAAAGGATTAGAACTAACACAAAACCTATCGGATTTTGCCGCTTTCTC
TCGCACCCGTCTTACGGAAGAGGCGTTATTTGGTGCAGACTTCCTAGTTCGAATGCAAAATCCGGCAGGTTTCTTTTATA
TGACCATCTTTGATAAGTGGAGTAAAGATATCAATCAACGTGATATTTGTGCTTATGCCACTCAAGAAGGTCATAAATCC
GATGATTATCAAGCAGGATTCCGCCAAGGTGGCGGCGTATCTATCGCTGCGCTAGCCGCTGCATCCCAATTAACACTTGA
TGGCGAATTTACTCGCGAAGAATACTTGGCCGCAGCAACTAAAGGCTACTGGCACTTAAAAGAAAACAACCTTAGTTATT
TAAATAATGGCGAAGAAAACATTATCGATGAATATTGTGCTCTTCTTGCTGTTATTGAACTATTTAAAGCAACACAAGAA
AATAACTTCTTAATTGAAGCTCGAATTTGGGCCGATAAACTTTCTGCTCGTCAACAAAGTGATGATAATTTCTCACACTT
CTGGTCAGCGACTGATAATGGCGAACGTCCATACTTTCACGCTGCAGAAGCGGGCCTTCCCGTTATTGCTCTCTCTCAAT
ACTTAGAAATAGAAACTGATAATAGTCGAAAAGCTCATACTCAATTAGTTATCAATCAAGCGGTTGAATTTGAACTGGCT
ATTTCATCTGAGACATTCAATCCATTTGGCTACCCAAGACAATACGTTAAAGCCGTTAATGGTCAAAAACGCAGTGCTTT
CTTTGTTGCTCACGACAATGAATCCGGCTACTGGTGGCAAGGCGAAAATGCTCGACTCGGATCTCTAGCGAGTATGAGCT
ACCTTGTGTTACCACACCTAACCTCTAACGAGTTAAAACAAAGACTAGCCGCATTTGCTCAACATAATCTGAATTGGGTA
CTAGGTCTAAATCCTTATGACATGTGTATGTTAGACGGCCATGGCCACAATAACCCTGATTACCTTCCTCATCTTGGTTT
CTTTAATGCCAAAGGTGGTGTTTGTAATGGAATTACTGGTGGTTTTGAAGACGAAGAAGATATTGCATTTAATCCTGCAC
CACATGGCGATGATATGTTGCAAAACTGGCGTTGGGGTGAGCAATGGATCCCTCATGGCGCATGGTATCTGTTAGCGATT
ATGTCTCAATCCGCTTATTACTCAGTTCAAGGAGCATAA

Upstream 100 bases:

>100_bases
AACAAAACTGTCAGACAACCATTTTGTTCGTTGTTACTTATACGAAAATTAATTTCATTTGTAATTCTGAACAGCAGTAA
TTGGTATTTTGGAGAAATAG

Downstream 100 bases:

>100_bases
TCATGACGCATTTATATGTCGGTATTGATGGTGGCGGTACATCTTGTCGAGCTCGTATTCGCAATGAACAAGGTGAATTA
TTAGGTGAAGCAAAAAGCGG

Product: endochitinase

Products: beta-D-Gal-(1->3)-D-GlcNAc; beta-D-Gal-(1->4)-D-Glc; lacto-N-biose; lactose

Alternate protein names: Endoglucanase-Related Protein; Endoglucanase-Like Protein; Chitobiase; Glycoside Hydrolase Family Protein; Endoglucanase; Glucosamine-Link Cellobiase; Beta-Glycosidase-Like Protein; Glycosy Hydrolase Family Protein

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MQLLTNHIGYETNGPKSAVLLATTALTQPISGHIVCTQTQEVKYRFSISSSDQVAHWHQGYFAEITFTDFTEEGEFILVI
EGAMSHSFVIKTNLLMAHTFSDVMHYFKSQRCGGIFDKQDKQAPLLNSNETVDVHGGWYDASGDVSKYLSHLSYANYLNP
QQIPMVVWNMLKGLELTQNLSDFAAFSRTRLTEEALFGADFLVRMQNPAGFFYMTIFDKWSKDINQRDICAYATQEGHKS
DDYQAGFRQGGGVSIAALAAASQLTLDGEFTREEYLAAATKGYWHLKENNLSYLNNGEENIIDEYCALLAVIELFKATQE
NNFLIEARIWADKLSARQQSDDNFSHFWSATDNGERPYFHAAEAGLPVIALSQYLEIETDNSRKAHTQLVINQAVEFELA
ISSETFNPFGYPRQYVKAVNGQKRSAFFVAHDNESGYWWQGENARLGSLASMSYLVLPHLTSNELKQRLAAFAQHNLNWV
LGLNPYDMCMLDGHGHNNPDYLPHLGFFNAKGGVCNGITGGFEDEEDIAFNPAPHGDDMLQNWRWGEQWIPHGAWYLLAI
MSQSAYYSVQGA

Sequences:

>Translated_572_residues
MQLLTNHIGYETNGPKSAVLLATTALTQPISGHIVCTQTQEVKYRFSISSSDQVAHWHQGYFAEITFTDFTEEGEFILVI
EGAMSHSFVIKTNLLMAHTFSDVMHYFKSQRCGGIFDKQDKQAPLLNSNETVDVHGGWYDASGDVSKYLSHLSYANYLNP
QQIPMVVWNMLKGLELTQNLSDFAAFSRTRLTEEALFGADFLVRMQNPAGFFYMTIFDKWSKDINQRDICAYATQEGHKS
DDYQAGFRQGGGVSIAALAAASQLTLDGEFTREEYLAAATKGYWHLKENNLSYLNNGEENIIDEYCALLAVIELFKATQE
NNFLIEARIWADKLSARQQSDDNFSHFWSATDNGERPYFHAAEAGLPVIALSQYLEIETDNSRKAHTQLVINQAVEFELA
ISSETFNPFGYPRQYVKAVNGQKRSAFFVAHDNESGYWWQGENARLGSLASMSYLVLPHLTSNELKQRLAAFAQHNLNWV
LGLNPYDMCMLDGHGHNNPDYLPHLGFFNAKGGVCNGITGGFEDEEDIAFNPAPHGDDMLQNWRWGEQWIPHGAWYLLAI
MSQSAYYSVQGA
>Mature_572_residues
MQLLTNHIGYETNGPKSAVLLATTALTQPISGHIVCTQTQEVKYRFSISSSDQVAHWHQGYFAEITFTDFTEEGEFILVI
EGAMSHSFVIKTNLLMAHTFSDVMHYFKSQRCGGIFDKQDKQAPLLNSNETVDVHGGWYDASGDVSKYLSHLSYANYLNP
QQIPMVVWNMLKGLELTQNLSDFAAFSRTRLTEEALFGADFLVRMQNPAGFFYMTIFDKWSKDINQRDICAYATQEGHKS
DDYQAGFRQGGGVSIAALAAASQLTLDGEFTREEYLAAATKGYWHLKENNLSYLNNGEENIIDEYCALLAVIELFKATQE
NNFLIEARIWADKLSARQQSDDNFSHFWSATDNGERPYFHAAEAGLPVIALSQYLEIETDNSRKAHTQLVINQAVEFELA
ISSETFNPFGYPRQYVKAVNGQKRSAFFVAHDNESGYWWQGENARLGSLASMSYLVLPHLTSNELKQRLAAFAQHNLNWV
LGLNPYDMCMLDGHGHNNPDYLPHLGFFNAKGGVCNGITGGFEDEEDIAFNPAPHGDDMLQNWRWGEQWIPHGAWYLLAI
MSQSAYYSVQGA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.14

Molecular weight: Translated: 64436; Mature: 64436

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLTNHIGYETNGPKSAVLLATTALTQPISGHIVCTQTQEVKYRFSISSSDQVAHWHQG
CCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCHHCCC
YFAEITFTDFTEEGEFILVIEGAMSHSFVIKTNLLMAHTFSDVMHYFKSQRCGGIFDKQD
EEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCC
KQAPLLNSNETVDVHGGWYDASGDVSKYLSHLSYANYLNPQQIPMVVWNMLKGLELTQNL
CCCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCH
SDFAAFSRTRLTEEALFGADFLVRMQNPAGFFYMTIFDKWSKDINQRDICAYATQEGHKS
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEHHHHHCCCCCCEEEEECCCCCCC
DDYQAGFRQGGGVSIAALAAASQLTLDGEFTREEYLAAATKGYWHLKENNLSYLNNGEEN
CCHHHHHCCCCCEEEEEEHHHHHEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHH
IIDEYCALLAVIELFKATQENNFLIEARIWADKLSARQQSDDNFSHFWSATDNGERPYFH
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCCCHHHEECCCCCCCCCEEE
AAEAGLPVIALSQYLEIETDNSRKAHTQLVINQAVEFELAISSETFNPFGYPRQYVKAVN
EHHCCCCCEEEEEEEEEECCCCCHHHHHHHEECCEEEEEEEECCCCCCCCCCHHHHHHHC
GQKRSAFFVAHDNESGYWWQGENARLGSLASMSYLVLPHLTSNELKQRLAAFAQHNLNWV
CCCCEEEEEEEECCCCCEECCCCCCCCCHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEE
LGLNPYDMCMLDGHGHNNPDYLPHLGFFNAKGGVCNGITGGFEDEEDIAFNPAPHGDDML
EECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHH
QNWRWGEQWIPHGAWYLLAIMSQSAYYSVQGA
HCCCCCHHCCCCHHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure
MQLLTNHIGYETNGPKSAVLLATTALTQPISGHIVCTQTQEVKYRFSISSSDQVAHWHQG
CCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCHHCCC
YFAEITFTDFTEEGEFILVIEGAMSHSFVIKTNLLMAHTFSDVMHYFKSQRCGGIFDKQD
EEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCC
KQAPLLNSNETVDVHGGWYDASGDVSKYLSHLSYANYLNPQQIPMVVWNMLKGLELTQNL
CCCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCH
SDFAAFSRTRLTEEALFGADFLVRMQNPAGFFYMTIFDKWSKDINQRDICAYATQEGHKS
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEHHHHHCCCCCCEEEEECCCCCCC
DDYQAGFRQGGGVSIAALAAASQLTLDGEFTREEYLAAATKGYWHLKENNLSYLNNGEEN
CCHHHHHCCCCCEEEEEEHHHHHEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHH
IIDEYCALLAVIELFKATQENNFLIEARIWADKLSARQQSDDNFSHFWSATDNGERPYFH
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCCCHHHEECCCCCCCCCEEE
AAEAGLPVIALSQYLEIETDNSRKAHTQLVINQAVEFELAISSETFNPFGYPRQYVKAVN
EHHCCCCCEEEEEEEEEECCCCCHHHHHHHEECCEEEEEEEECCCCCCCCCCHHHHHHHC
GQKRSAFFVAHDNESGYWWQGENARLGSLASMSYLVLPHLTSNELKQRLAAFAQHNLNWV
CCCCEEEEEEEECCCCCEECCCCCCCCCHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEE
LGLNPYDMCMLDGHGHNNPDYLPHLGFFNAKGGVCNGITGGFEDEEDIAFNPAPHGDDML
EECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHH
QNWRWGEQWIPHGAWYLLAIMSQSAYYSVQGA
HCCCCCHHCCCCHHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc; H2O; lacto-N-tetraose

Specific reaction: (1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O = beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc; (2) lacto-N-tetraose + H2O = lacto-N-biose + lactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA