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The map label for this gene is yfiD [H]

Identifier: 59712722

GI number: 59712722

Start: 2349844

End: 2350221

Strand: Direct

Name: yfiD [H]

Synonym: VF_2115

Alternate gene names: 59712722

Gene position: 2349844-2350221 (Clockwise)

Preceding gene: 59712718

Following gene: 59712727

Centisome position: 81.1

GC content: 41.01

Gene sequence:

>378_bases
ATGATCACAGGTATCCAAATTACTAAAGCAGCAAATGACGATCTTCTTAACTCAATCTGGCTACTAGATAGCGAGAAAAA
CGAAGCACGTTGTGTTGTTGCAACAGCAGGTTTTGAAGCTGACCAAGTTATCGCAGCAAGCGAACTAGGTGAGTACGAAA
GCCGTGATGTTGCTATCGAAAAAGCACCAAAAATTGAAGGCGGTCAGCACCTAAATGTTAACGTTTTACAACGTGACACT
CTAGAAGATGCTGTTAAGCACCCAGAGAACTACCCACAACTAACTATCCGTGTATCTGGTTATGCTGTGCGTTTTAACTC
TCTAACAACAGAACAACAAAAAGACGTTATCGCACGTACTTTTACTGAATCTCTATAA

Upstream 100 bases:

>100_bases
TTTTGACATATATCAATATACAAAGTAAGGGTATCATGTATATAATGTGACCCATAAGATATTTAGAAAATAACCACAAA
GTGGACTAGGAGAAAGTAAA

Downstream 100 bases:

>100_bases
TTTTATAGTCGATTTAAAAATACGAAACGGCACTCAATGCTCATGAGTGCCGTTTTTTTATGCCAATCACAGTAAGTATT
TAACTGCTAGGGTGCTCAAT

Product: autonomous glycyl radical cofactor GrcA

Products: CoA; pyruvate

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATAGFEADQVIAASELGEYESRDVAIEKAPKIEGGQHLNVNVLQRDT
LEDAVKHPENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL

Sequences:

>Translated_125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATAGFEADQVIAASELGEYESRDVAIEKAPKIEGGQHLNVNVLQRDT
LEDAVKHPENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL
>Mature_125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATAGFEADQVIAASELGEYESRDVAIEKAPKIEGGQHLNVNVLQRDT
LEDAVKHPENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins [H]

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain [H]

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=77.9527559055118, Blast_Score=208, Evalue=9e-56,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=77.0491803278689, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=73.7704918032787, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1787044, Length=57, Percent_Identity=43.859649122807, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140 [H]

Pfam domain/function: PF01228 Gly_radical [H]

EC number: 2.3.1.54

Molecular weight: Translated: 13848; Mature: 13848

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATAGFEADQVIAASELGEYESRDVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCCCCEEEHHHCCCCCCCCEEEE
KAPKIEGGQHLNVNVLQRDTLEDAVKHPENYPQLTIRVSGYAVRFNSLTTEQQKDVIART
CCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC
>Mature Secondary Structure
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATAGFEADQVIAASELGEYESRDVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCCCCEEEHHHCCCCCCCCEEEE
KAPKIEGGQHLNVNVLQRDTLEDAVKHPENYPQLTIRVSGYAVRFNSLTTEQQKDVIART
CCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA