Click here to switch to the map view.

The map label for this gene is ubiH [H]

Identifier: 59712708

GI number: 59712708

Start: 2336721

End: 2337920

Strand: Reverse

Name: ubiH [H]

Synonym: VF_2101

Alternate gene names: 59712708

Gene position: 2337920-2336721 (Counterclockwise)

Preceding gene: 59712709

Following gene: 59712707

Centisome position: 80.69

GC content: 40.58

Gene sequence:

>1200_bases
ATGGATTCTAGAATGACACATTATGATGTAGTTATTGTTGGTGGTGCGATGGCGGGAGCAACACTCGCTTTAGCATTAGA
AAAAATGAATCAAGGGCGTCTTTCGATTGCGGTTATTGAAGCTGTCGAACCAAAACTTGATTCTCACCCAGGATATGATT
CTCGAAGTATTGCTCTTTCTAATGGAACTGCGCAGCTATTGAAAGACATTGCTTTGTGGTCAGATATCGCCCCGTTAGCA
ACACCAATAAAGCATATTCACATCTCAGATCAGCAACATGCAGGAATAACAGAGTTAACATCAAAAGAATTGAACGTTGA
TGATCTTGGTTATGTTGTTGAGCTTGCTGATATTGGTGATTTTTATCATAAAAAATTAATAAACTCTTCAGATATAGAAC
TTATTTGCCCTGCATCGGTTTCTCATCTTGAGCGAGAGTTAGCACTGACTAAGATAACGCTCTCTACAGGAAAGGTTATT
TCTGCATCTTTGGTTGTTGCAGCAGATGGCGCTGAATCTGCCACGTGCAAAAGCATGCAATTAGAATCTGAAGTGTTTGA
TTTTGAGCAGGTCGCTATCATCGCTAATGTGACAACAGAGCTCCATCCTCAAGGAAAAGCATTTGAGCGTTTTACTGAGC
ATGGCCCTGTCGCTCTCTTGCCGATGTCTAATGGTCGTAGCTCACTTGTATGGTGTGTTACTCCTGAAGCATCGCAGGAA
ATATTAGAGTACAGTGATGAGCAATTTTTAGCGCATCTACAACATGAATTTGGTTGGCGCCTTGGTAAGTTAATAAAAAC
GGGTAAGCGAGCACATTATCCTTTGTTACTTCGTCGTCAAAAACAGCATGTTTCCCATCGTTTTGCTGCTATTGGTAACG
CAGCCCAAACTCTGCATCCGATTGCAGGCCAAGGGTTTAACCTTGGAATAAGAGATGTATTTTCATTAAGTGAATGTATT
ACTCAAGCTTCTTTGAATGGTCAAGATATTGGTGATTATTCGGTGCTAGCTGAGTATCAGAATCGACGAGAACCAGACCA
ACAGCAAACCATAACGATGACTGCAGGATTAATTTCTATCTTTGCAACTCATTCATTCCCAATGGTAATTGGACGAAATA
TTGGTTTGTATGCGGTTGATACTTTCCGTTTTTTATTAAAACCCGCGACATTACGAGCAATGGGTAGAGTGAAAAGATAA

Upstream 100 bases:

>100_bases
TCTTCGAACAAATAGTAGAGCATGTGCGCATTTGTGTTCTTACTATTCATGCTGAATTAGGTCAGCAAATACATACCGAT
GCATCTAAAACAGTGCATTA

Downstream 100 bases:

>100_bases
TTATGATGCAGAGTGTAGATATTGCGATTATTGGTGGCGGTATGGTTGGTTTAACCGTAGCCGCTGCATTAGAAAACAGT
GGATTAAGAATCGCAGTCAT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MDSRMTHYDVVIVGGAMAGATLALALEKMNQGRLSIAVIEAVEPKLDSHPGYDSRSIALSNGTAQLLKDIALWSDIAPLA
TPIKHIHISDQQHAGITELTSKELNVDDLGYVVELADIGDFYHKKLINSSDIELICPASVSHLERELALTKITLSTGKVI
SASLVVAADGAESATCKSMQLESEVFDFEQVAIIANVTTELHPQGKAFERFTEHGPVALLPMSNGRSSLVWCVTPEASQE
ILEYSDEQFLAHLQHEFGWRLGKLIKTGKRAHYPLLLRRQKQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLSECI
TQASLNGQDIGDYSVLAEYQNRREPDQQQTITMTAGLISIFATHSFPMVIGRNIGLYAVDTFRFLLKPATLRAMGRVKR

Sequences:

>Translated_399_residues
MDSRMTHYDVVIVGGAMAGATLALALEKMNQGRLSIAVIEAVEPKLDSHPGYDSRSIALSNGTAQLLKDIALWSDIAPLA
TPIKHIHISDQQHAGITELTSKELNVDDLGYVVELADIGDFYHKKLINSSDIELICPASVSHLERELALTKITLSTGKVI
SASLVVAADGAESATCKSMQLESEVFDFEQVAIIANVTTELHPQGKAFERFTEHGPVALLPMSNGRSSLVWCVTPEASQE
ILEYSDEQFLAHLQHEFGWRLGKLIKTGKRAHYPLLLRRQKQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLSECI
TQASLNGQDIGDYSVLAEYQNRREPDQQQTITMTAGLISIFATHSFPMVIGRNIGLYAVDTFRFLLKPATLRAMGRVKR
>Mature_399_residues
MDSRMTHYDVVIVGGAMAGATLALALEKMNQGRLSIAVIEAVEPKLDSHPGYDSRSIALSNGTAQLLKDIALWSDIAPLA
TPIKHIHISDQQHAGITELTSKELNVDDLGYVVELADIGDFYHKKLINSSDIELICPASVSHLERELALTKITLSTGKVI
SASLVVAADGAESATCKSMQLESEVFDFEQVAIIANVTTELHPQGKAFERFTEHGPVALLPMSNGRSSLVWCVTPEASQE
ILEYSDEQFLAHLQHEFGWRLGKLIKTGKRAHYPLLLRRQKQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLSECI
TQASLNGQDIGDYSVLAEYQNRREPDQQQTITMTAGLISIFATHSFPMVIGRNIGLYAVDTFRFLLKPATLRAMGRVKR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=375, Percent_Identity=26.9333333333333, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI32967307, Length=321, Percent_Identity=28.3489096573209, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1789274, Length=390, Percent_Identity=46.4102564102564, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786883, Length=394, Percent_Identity=31.7258883248731, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789273, Length=395, Percent_Identity=31.1392405063291, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25146631, Length=423, Percent_Identity=26.4775413711584, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321694, Length=439, Percent_Identity=25.5125284738041, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19920760, Length=413, Percent_Identity=25.4237288135593, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43780; Mature: 43780

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRMTHYDVVIVGGAMAGATLALALEKMNQGRLSIAVIEAVEPKLDSHPGYDSRSIALS
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEC
NGTAQLLKDIALWSDIAPLATPIKHIHISDQQHAGITELTSKELNVDDLGYVVELADIGD
CCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHCCCCCCCCCCHHEEEEHHHHH
FYHKKLINSSDIELICPASVSHLERELALTKITLSTGKVISASLVVAADGAESATCKSMQ
HHHHHHCCCCCCEEECCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCCCCHHHHHH
LESEVFDFEQVAIIANVTTELHPQGKAFERFTEHGPVALLPMSNGRSSLVWCVTPEASQE
HHHHHHCHHHEEEEEECHHCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHH
ILEYSDEQFLAHLQHEFGWRLGKLIKTGKRAHYPLLLRRQKQHVSHRFAAIGNAAQTLHP
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCC
IAGQGFNLGIRDVFSLSECITQASLNGQDIGDYSVLAEYQNRREPDQQQTITMTAGLISI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHEEEHHHHHHHH
FATHSFPMVIGRNIGLYAVDTFRFLLKPATLRAMGRVKR
HHHCCCCEEECCCCCEEEHHHHHHHHCHHHHHHHHCCCC
>Mature Secondary Structure
MDSRMTHYDVVIVGGAMAGATLALALEKMNQGRLSIAVIEAVEPKLDSHPGYDSRSIALS
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEC
NGTAQLLKDIALWSDIAPLATPIKHIHISDQQHAGITELTSKELNVDDLGYVVELADIGD
CCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHCCCCCCCCCCHHEEEEHHHHH
FYHKKLINSSDIELICPASVSHLERELALTKITLSTGKVISASLVVAADGAESATCKSMQ
HHHHHHCCCCCCEEECCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCCCCHHHHHH
LESEVFDFEQVAIIANVTTELHPQGKAFERFTEHGPVALLPMSNGRSSLVWCVTPEASQE
HHHHHHCHHHEEEEEECHHCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHH
ILEYSDEQFLAHLQHEFGWRLGKLIKTGKRAHYPLLLRRQKQHVSHRFAAIGNAAQTLHP
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCC
IAGQGFNLGIRDVFSLSECITQASLNGQDIGDYSVLAEYQNRREPDQQQTITMTAGLISI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHEEEHHHHHHHH
FATHSFPMVIGRNIGLYAVDTFRFLLKPATLRAMGRVKR
HHHCCCCEEECCCCCEEEHHHHHHHHCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]