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The map label for this gene is dcuB [H]

Identifier: 59712671

GI number: 59712671

Start: 2299566

End: 2300798

Strand: Direct

Name: dcuB [H]

Synonym: VF_2064

Alternate gene names: 59712671

Gene position: 2299566-2300798 (Clockwise)

Preceding gene: 59712670

Following gene: 59712673

Centisome position: 79.36

GC content: 40.96

Gene sequence:

>1233_bases
ATGTTGTATTTTGAATTTCTCTTTTTATTACTCGTTCTCTATATCGGTTCTCGATATGGAGGAATTGGATTAGGGGTTGT
ATCTGGTATTGGTTTGGTGATTGAAGTATTCATCTTCAAAATGCCACCGACCTCTCCGCCCGTTACGGTAATGCTGATCA
TCTTGGCGGTTGTTACCTGTGCTTCTATATTAGAAGCTGCCGGTGGTTTAAAGTACATGCTGCAAGTTGCTGAGCGGATT
TTAAGAAAGAATCCAAAACGCGTCACCATCATTGCTCCATTTGTTACTTATGCGATGACATTCTTACTTGGTACGGGTCA
TGCTGTTTATTCAATTATGCCTATCATTGGTGATGTTGCATTAAAGAATGGTATTCGTCCTGAGCGTCCTATGGCTGCCG
CATCTGTTGCGTCTCAAATCGCAATTACTGCTTCACCATTATCTGCCGCAGTTGTGTATTACCTAGCTCAACTGGTTGAT
ATTGATGCTTCTATTACATTAATGTCGATTTTAATGGTTACTGTACCTGCAACGTTATTCGGTACTTTAATGCTGTCTCT
TTATAGCTTACGTCGCGGTAAAGAATTGGAAGATGATGAAGAGTATCAAGCACGTTTACAAGATCCGGTTTGGCGTGAAA
AAATCCTAAATACAACATCAACATCTCTTGATGAAGAGCTACCAACAACAGCTCGTAATGCTGTTCTGATCTTCTTAGGT
GCCATCGTTTCTATCGTGATTATAGCGATGTGGCCAGAAGTGCGCACCATTACTGATGGCGGTGCACCAATCAAAATGTC
TGTTGTAATTCAAATGATGATGTTGGCTTTTGGTGGTGTGATTTTACTTGCAACGAAAACCGATCCTCGTGATGTTCCAA
ATGGCGTTGTGTTTAAATCAGGAATGGTGGCGGCAATTGCTATTTTTGGTATCGCATGGATGTCAGATACCTATTTCCAA
TATGCCATGCCTCAATTTAGAGCCGGTATTGTAGATATGGTAACGCTTCACCCTTGGACATTCGCTTTATCACTATTTAT
TGTATCTGTTGTGGTTAACTCACAAGCGGCAACAGCTCGTATGATGCTACCAGTTGGTTTAGCTCTGGGTTTAGAGCCTG
CACTGGTTATTGGTTTAATGCCAGCAGTATATGGATACTTCTTTATTCCTAACTACCCATCAGATATTGCAACCGTAAAC
TTTGATAGCTCGGGTACAACTAAAAAATGGTAA

Upstream 100 bases:

>100_bases
ACAACCTAAATGAGAACAAGCTCAATAAAACCCTATGAAATGCAAGGTATTAATAGCCTTAGTTGATTTTAATTATAATT
GCGTTAACGCAGGTGGTTGT

Downstream 100 bases:

>100_bases
GTACTACTTTAACCACTCATTCATGTCAGTCGGTTTAATCGGCGTGGTTTCAGCCTGTATTCTTGGCTATGTCTTAGGCC
AAATTGTGATTGGTTAATTC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLYFEFLFLLLVLYIGSRYGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQVAERI
LRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVD
IDASITLMSILMVTVPATLFGTLMLSLYSLRRGKELEDDEEYQARLQDPVWREKILNTTSTSLDEELPTTARNAVLIFLG
AIVSIVIIAMWPEVRTITDGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMSDTYFQ
YAMPQFRAGIVDMVTLHPWTFALSLFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVN
FDSSGTTKKW

Sequences:

>Translated_410_residues
MLYFEFLFLLLVLYIGSRYGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQVAERI
LRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVD
IDASITLMSILMVTVPATLFGTLMLSLYSLRRGKELEDDEEYQARLQDPVWREKILNTTSTSLDEELPTTARNAVLIFLG
AIVSIVIIAMWPEVRTITDGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMSDTYFQ
YAMPQFRAGIVDMVTLHPWTFALSLFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVN
FDSSGTTKKW
>Mature_410_residues
MLYFEFLFLLLVLYIGSRYGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQVAERI
LRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVD
IDASITLMSILMVTVPATLFGTLMLSLYSLRRGKELEDDEEYQARLQDPVWREKILNTTSTSLDEELPTTARNAVLIFLG
AIVSIVIIAMWPEVRTITDGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMSDTYFQ
YAMPQFRAGIVDMVTLHPWTFALSLFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVN
FDSSGTTKKW

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=375, Percent_Identity=46.6666666666667, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1790580, Length=409, Percent_Identity=34.4743276283619, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 44471; Mature: 44471

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYFEFLFLLLVLYIGSRYGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTC
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASILEAAGGLKYMLQVAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVDIDASITLMSILMVTVPATLF
HHCCCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GTLMLSLYSLRRGKELEDDEEYQARLQDPVWREKILNTTSTSLDEELPTTARNAVLIFLG
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
AIVSIVIIAMWPEVRTITDGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKS
HHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECC
GMVAAIAIFGIAWMSDTYFQYAMPQFRAGIVDMVTLHPWTFALSLFIVSVVVNSQAATAR
HHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHH
MMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVNFDSSGTTKKW
HHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MLYFEFLFLLLVLYIGSRYGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTC
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASILEAAGGLKYMLQVAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVDIDASITLMSILMVTVPATLF
HHCCCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GTLMLSLYSLRRGKELEDDEEYQARLQDPVWREKILNTTSTSLDEELPTTARNAVLIFLG
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
AIVSIVIIAMWPEVRTITDGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKS
HHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECC
GMVAAIAIFGIAWMSDTYFQYAMPQFRAGIVDMVTLHPWTFALSLFIVSVVVNSQAATAR
HHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHH
MMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVNFDSSGTTKKW
HHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]