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The map label for this gene is phoB [H]

Identifier: 59712595

GI number: 59712595

Start: 2221952

End: 2222644

Strand: Reverse

Name: phoB [H]

Synonym: VF_1988

Alternate gene names: 59712595

Gene position: 2222644-2221952 (Counterclockwise)

Preceding gene: 59712599

Following gene: 59712594

Centisome position: 76.71

GC content: 41.7

Gene sequence:

>693_bases
ATGGCTAGAAGGATCCTTGTTGTAGAAGATGAAGCACCAATTCGTGAAATGTTGTGTTTTGTACTAGAGCAAAAAGGTTA
TGAACCTATCGAAGCAGAAGATTATGACTCTGCGCTATCCCTACTTTGTGAACCTTATCCTGATCTAATCTTGCTAGATT
GGATGCTACCTGGTGGTTCAGGCATTAATTTAATAAAACACCTTAAGCGTGATGAGTTTACAAAAAAGATCCCTGTGGTC
ATGTTAACCGCTCGCGGTGAAGAAGAAGACAAGGTTCGTGGCCTAGAAGTGGGCGCGGATGATTACATCACCAAACCATT
TTCACCAAAGGAATTAATGGCACGTTTAAAAGCGGTAATTCGTCGTGTCTCTCCAACATCAATCGAAGAGCTGATTGATG
TACAAGGTCTTAAACTTGATCCTGTATCTCATCGTGTGACGGCAAATGATAAGCCGCTAGATATGGGACCGACTGAGTTT
AAAATGCTGCATTTCTTTATGACACACCAAGAACGTGTATACAGTCGTGAACAGTTATTAAATAACGTATGGGGCACGAA
TGTGTATGTAGAAGATAGAACGGTAGATGTTCATATTCGTCGTTTACGTAAAGCGTTGGAAGTAGAAGGTCATGATCGTT
TAGTTCAAACGGTTCGTGGTGCGGGCTATCGCTTCTCTGTACAAGCGTCATAA

Upstream 100 bases:

>100_bases
GCATAGAATATGAACTTTTCTTTTCAATAGTCACAAAAGTGTCATAATTAGCTTTAATAATAGCTTCACAATTTTTGTTG
GTTAACTTAGGTAAAAGAGA

Downstream 100 bases:

>100_bases
TTTAATTATTGTAAGGTATAAAGGTAATGGTTGAGCGTCTTTCATGGAAAAAATTGATTGGAGAATTGGTTTTTTTCTAT
GTTCCTTGGGTTATTGTAGG

Product: DNA-binding response regulator in two-component regulatory system with PhoR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MARRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDSALSLLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSIEELIDVQGLKLDPVSHRVTANDKPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEVEGHDRLVQTVRGAGYRFSVQAS

Sequences:

>Translated_230_residues
MARRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDSALSLLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSIEELIDVQGLKLDPVSHRVTANDKPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEVEGHDRLVQTVRGAGYRFSVQAS
>Mature_229_residues
ARRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDSALSLLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVVM
LTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSIEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFK
MLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEVEGHDRLVQTVRGAGYRFSVQAS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=80.2631578947368, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=39.7379912663755, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.8078602620087, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=36.1607142857143, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=34.6846846846847, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.322869955157, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=37.0535714285714, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.4444444444444, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI2367329, Length=236, Percent_Identity=37.2881355932203, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=35.6223175965665, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=33.4841628959276, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.7391304347826, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=29.8319327731092, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=37.6146788990826, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26420; Mature: 26289

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDSALSLLCEPYPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
GINLIKHLKRDEFTKKIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVI
CHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVSPTSIEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCCCHHHHHHHCCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEVEGHDRLVQTVRGAGYRFSVQAS
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
ARRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDSALSLLCEPYPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
GINLIKHLKRDEFTKKIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVI
CHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVSPTSIEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCCCHHHHHHHCCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEVEGHDRLVQTVRGAGYRFSVQAS
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]