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The map label for this gene is narQ [H]

Identifier: 59712515

GI number: 59712515

Start: 2140164

End: 2141855

Strand: Direct

Name: narQ [H]

Synonym: VF_1908

Alternate gene names: 59712515

Gene position: 2140164-2141855 (Clockwise)

Preceding gene: 59712508

Following gene: 59712516

Centisome position: 73.86

GC content: 35.76

Gene sequence:

>1692_bases
ATGAAACATAACAAGACCTCAATCTTAACAAGAACCATTGCTCAAGTGATGTTGTTGATTGTTATGATCTCCATAATTAC
AACGAGCTTAGCTTTAATCACTCTTTCCTCTAGCTTAAAAGATGCAGAAGCAGTCAATATTGCTGGTTCATTACGTATGC
AGAGCTATCGTTTAGCTTATGATATTGAAACAAACTCCCCAGAGCTCATTGAGCACTTAGATAAATTTTCACAATCAATA
GAATCGCCGTCATTTAAATCATTAGACCAATGGTTTGTACCTGATGACATTGAAGATTATTACGAAGATATCCGACTACA
ATGGCTTTCCTTACAGCCATCACTATTAAGCGACAACAAACAAATTTATTTAAATAAAGTTTCCCTTTTCGTTGATGAAA
TTGATCATTTTGTTTTTCGTTTACAAGAGTTTTCAGAAAGAAAACTTCAATTACTTTCTCTGATTGGTGCTCTTGGTTTA
TCACTGATTGTCTTCTCAAGTATTTTTATTATTTTTTTCACTCAAAGAAAAATCGTGAGCCCATTACATCACCTAGTTGC
GGCAAGCCATGCCATGACAAAAGGAAACTATTCAGTTCAGGTGGATTTAAATAGTGATAATGAATTAGGTCAGCTAGGAA
ATGCATTTAATCACATGACAAGGCAGGTCGATTTAAGCTATCGAGAATTAGAAAGTCGAGTTGAAGATAAAACCAAGAAA
CTCAGCCAAGCCAATCGCTCATTAATGATCTTGTATCAATGCTCTCAACAACTCTCAGCGTCACAACTGGATGAACAAGC
GTTTAAAAACATTCTTGATACCTTTACGAATATTGAAGGGATTATCAGCGCGCGTCTGATTGTTGAAGAGGAATCCGGAG
GGGATTGGGAGATAACAAGCGGTGAGCCTGACGAGTCCCCATGGAGCCTTCAGGAGCTATGTATTGATGGCGAACAATTA
GGATACTTATTATGGCAAGAATCATTGCCTTGCCCTGATCAGAAATTAATCATCAATATCTCAAATATTCTGGCGCGTGG
TATTTTCTATAACCAAGCACAAAAACAAACACACCAACTCATTTTATTAGAAGAGCGCTCAACAATAGCTCGTGAGCTTC
ATGATTCTTTAGCTCAGTCGCTTTCCTATTTGAAAATTCAAACAACACTATTAAAACGACAATTAGAAAAGTGTGATTGT
ATAAATACAAGTACTACACTAGTAGAATTAGATGAAGGTCTTAAAAGTGCATATAGTCAATTACGAGGACTATTAAATAC
ATTTAGATTAACCATTAATAAAGCTCACTTTGGTGAGGCCTTACAAGAAATCATGACAACCTTAGCCTCACAAACTAAGA
TAAATATTCATTTAAATAACGAACTTCCTTCTTTGCCGATAAATGCTCAACAACATGTTCATTTACTACAATTGATAAGA
GAAGCGACATTAAATGCCATCAAACACTCAAAAGCTGATAATATTATCATTACGTGTTTTCAAGAAGGTGAGCAAGGCTG
TATTAACATTGAAGACGATGGAGTAGGATTTGACCCTACAGAAGAAAAAATAAATCATTATGGGTTAAGGATCATGCAAG
AACGTGCAAACTGCGTTCATGGTAAGCTATCCATAACTTCAGAAATAGACAACGGTTGTACAGTGAACGTTATGTTTCCA
CTAAACCAATAA

Upstream 100 bases:

>100_bases
AACATAGGTAAATTTTGTTTTAGATCAATTTTTAAAATAAATAAAAACAACAAAATAGATCCCATTTGCTTCTTTTAATC
ATAGGTCACAATTAACAAAC

Downstream 100 bases:

>100_bases
AATAATAGAGGTAAAAAATTATGTGGAATGTCGTTATCGTTGATGATCATCCGTTAATGCGTCGTGGTATTGGTCAACTT
CTTTCTTTTGATGAAGAATT

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKHNKTSILTRTIAQVMLLIVMISIITTSLALITLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPELIEHLDKFSQSI
ESPSFKSLDQWFVPDDIEDYYEDIRLQWLSLQPSLLSDNKQIYLNKVSLFVDEIDHFVFRLQEFSERKLQLLSLIGALGL
SLIVFSSIFIIFFTQRKIVSPLHHLVAASHAMTKGNYSVQVDLNSDNELGQLGNAFNHMTRQVDLSYRELESRVEDKTKK
LSQANRSLMILYQCSQQLSASQLDEQAFKNILDTFTNIEGIISARLIVEEESGGDWEITSGEPDESPWSLQELCIDGEQL
GYLLWQESLPCPDQKLIINISNILARGIFYNQAQKQTHQLILLEERSTIARELHDSLAQSLSYLKIQTTLLKRQLEKCDC
INTSTTLVELDEGLKSAYSQLRGLLNTFRLTINKAHFGEALQEIMTTLASQTKINIHLNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKADNIIITCFQEGEQGCINIEDDGVGFDPTEEKINHYGLRIMQERANCVHGKLSITSEIDNGCTVNVMFP
LNQ

Sequences:

>Translated_563_residues
MKHNKTSILTRTIAQVMLLIVMISIITTSLALITLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPELIEHLDKFSQSI
ESPSFKSLDQWFVPDDIEDYYEDIRLQWLSLQPSLLSDNKQIYLNKVSLFVDEIDHFVFRLQEFSERKLQLLSLIGALGL
SLIVFSSIFIIFFTQRKIVSPLHHLVAASHAMTKGNYSVQVDLNSDNELGQLGNAFNHMTRQVDLSYRELESRVEDKTKK
LSQANRSLMILYQCSQQLSASQLDEQAFKNILDTFTNIEGIISARLIVEEESGGDWEITSGEPDESPWSLQELCIDGEQL
GYLLWQESLPCPDQKLIINISNILARGIFYNQAQKQTHQLILLEERSTIARELHDSLAQSLSYLKIQTTLLKRQLEKCDC
INTSTTLVELDEGLKSAYSQLRGLLNTFRLTINKAHFGEALQEIMTTLASQTKINIHLNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKADNIIITCFQEGEQGCINIEDDGVGFDPTEEKINHYGLRIMQERANCVHGKLSITSEIDNGCTVNVMFP
LNQ
>Mature_563_residues
MKHNKTSILTRTIAQVMLLIVMISIITTSLALITLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPELIEHLDKFSQSI
ESPSFKSLDQWFVPDDIEDYYEDIRLQWLSLQPSLLSDNKQIYLNKVSLFVDEIDHFVFRLQEFSERKLQLLSLIGALGL
SLIVFSSIFIIFFTQRKIVSPLHHLVAASHAMTKGNYSVQVDLNSDNELGQLGNAFNHMTRQVDLSYRELESRVEDKTKK
LSQANRSLMILYQCSQQLSASQLDEQAFKNILDTFTNIEGIISARLIVEEESGGDWEITSGEPDESPWSLQELCIDGEQL
GYLLWQESLPCPDQKLIINISNILARGIFYNQAQKQTHQLILLEERSTIARELHDSLAQSLSYLKIQTTLLKRQLEKCDC
INTSTTLVELDEGLKSAYSQLRGLLNTFRLTINKAHFGEALQEIMTTLASQTKINIHLNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKADNIIITCFQEGEQGCINIEDDGVGFDPTEEKINHYGLRIMQERANCVHGKLSITSEIDNGCTVNVMFP
LNQ

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=557, Percent_Identity=41.113105924596, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787474, Length=595, Percent_Identity=27.7310924369748, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87082311, Length=197, Percent_Identity=25.8883248730964, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63818; Mature: 63818

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNKTSILTRTIAQVMLLIVMISIITTSLALITLSSSLKDAEAVNIAGSLRMQSYRLAY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEE
DIETNSPELIEHLDKFSQSIESPSFKSLDQWFVPDDIEDYYEDIRLQWLSLQPSLLSDNK
EECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHEEEEECCCHHHCCCC
QIYLNKVSLFVDEIDHFVFRLQEFSERKLQLLSLIGALGLSLIVFSSIFIIFFTQRKIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLHHLVAASHAMTKGNYSVQVDLNSDNELGQLGNAFNHMTRQVDLSYRELESRVEDKTKK
HHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LSQANRSLMILYQCSQQLSASQLDEQAFKNILDTFTNIEGIISARLIVEEESGGDWEITS
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEECC
GEPDESPWSLQELCIDGEQLGYLLWQESLPCPDQKLIINISNILARGIFYNQAQKQTHQL
CCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCE
ILLEERSTIARELHDSLAQSLSYLKIQTTLLKRQLEKCDCINTSTTLVELDEGLKSAYSQ
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRGLLNTFRLTINKAHFGEALQEIMTTLASQTKINIHLNNELPSLPINAQQHVHLLQLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EATLNAIKHSKADNIIITCFQEGEQGCINIEDDGVGFDPTEEKINHYGLRIMQERANCVH
HHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKLSITSEIDNGCTVNVMFPLNQ
CCEEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MKHNKTSILTRTIAQVMLLIVMISIITTSLALITLSSSLKDAEAVNIAGSLRMQSYRLAY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEE
DIETNSPELIEHLDKFSQSIESPSFKSLDQWFVPDDIEDYYEDIRLQWLSLQPSLLSDNK
EECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHEEEEECCCHHHCCCC
QIYLNKVSLFVDEIDHFVFRLQEFSERKLQLLSLIGALGLSLIVFSSIFIIFFTQRKIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLHHLVAASHAMTKGNYSVQVDLNSDNELGQLGNAFNHMTRQVDLSYRELESRVEDKTKK
HHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LSQANRSLMILYQCSQQLSASQLDEQAFKNILDTFTNIEGIISARLIVEEESGGDWEITS
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEECC
GEPDESPWSLQELCIDGEQLGYLLWQESLPCPDQKLIINISNILARGIFYNQAQKQTHQL
CCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCE
ILLEERSTIARELHDSLAQSLSYLKIQTTLLKRQLEKCDCINTSTTLVELDEGLKSAYSQ
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRGLLNTFRLTINKAHFGEALQEIMTTLASQTKINIHLNNELPSLPINAQQHVHLLQLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EATLNAIKHSKADNIIITCFQEGEQGCINIEDDGVGFDPTEEKINHYGLRIMQERANCVH
HHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKLSITSEIDNGCTVNVMFPLNQ
CCEEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]