Definition Vibrio fischeri ES114 chromosome I, complete genome.
Accession NC_006840
Length 2,897,536

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The map label for this gene is napA

Identifier: 59712512

GI number: 59712512

Start: 2136688

End: 2139177

Strand: Reverse

Name: napA

Synonym: VF_1905

Alternate gene names: 59712512

Gene position: 2139177-2136688 (Counterclockwise)

Preceding gene: 59712513

Following gene: 59712511

Centisome position: 73.83

GC content: 41.97

Gene sequence:

>2490_bases
ATGAAAATGACAAGACGTGCGTTTGTTAAAGCAAACGCAGCAGCTTCGGCTGCAGCTGTTGCAGGGGTTACGCTGCCTGC
TTCAGCAACAAATTTAATCGTTAGTTCTGACCAAACAAAAATTAAATGGGATAAAGCGCCTTGTCGTTTTTGTGGTACAG
GTTGTTCAGTACTTGTTGGTACTCAAAATGGTCGCGTAGTCGCTACTCAAGGTGATCCTGAAGCACCTGTAAACAAAGGT
CTTAACTGTATTAAAGGTTATTTCCTTTCTAAGATTATGTACGGTAAAGATCGTGTTCAAACGCCAATGTTGCGTATGAA
AGACGGCCAATATAACAAAGATGGTGACTTTGCTCCTGTATCTTGGGATGTTGCTCTAGATACCATGGCTGAAAAATGGA
AAGCGGCTCTGAAAAAACATGGTCCAACAGGTGTTGGTATGTTTGGTTCTGGTCAGTGGACAGTAATGGAAGGCTATGCA
GCGGTTAAGCTGATGAAAGCGGGTTTCCGTTCAAACAACATTGATCCGAATGCTCGTCACTGTATGGCATCTGCTGTAGG
TGCATTTATGCGTACATTTGGTATCGATGAGCCTATGGGTTGTTATGATGATTTTGAAAACGCAGACTCGTTTGTTCTGT
GGGGTTCAAACATGGCAGAAATGCACCCAGTATTATGGACTCGCATTTCAGACCGTCGTTTAAGTCATCCTCATGTAAAA
GTTAATGTATTATCAACGTACTACCATCGCTCATTTGAATTAGCAGATAAAGGCTATATTTTTGAACCTCAATCTGACCT
TGCTATTGCTAACTTCATTGCTAACTACATTATCCAAAATAATGCAGTTAACTGGGATTTCGTTAATAAACATACGAACT
TCAAACAAGCGACGACGGATATTGGTTACGGTTTACGTGATGATGATCCTCTTCAAATGGAAGCGGCAAACCCTAACTCA
GGTGCAATGTCTTCAATTAGCTTTGAAGAGTATAAAAAATCAGTAGCTCCTTATACGGCACAAAAAGCATCTGAAATGTC
AGGCGTATCGGAAGAAGATCTGATTACTCTAGCTAAACAATATGCAGATCCAAAAACAAAAGTAATGTCTTTATGGACAA
TGGGTATGAACCAACATACTCGTGGTGTTTGGATGAACAGCCTTGTTTATAACATTCACTTGCTTACAGGTAAAATCGCA
ACACCTGGTAACAGCCCATTCTCTCTTACAGGTCAACCATCTGCATGTGGTACTGCTCGTGAAGTTGGTACGTTCTCGCA
TCGTCTACCTGCTGATATGGTGGTTGCTAACCCTAAACACCGTGCTATCTCTGAGAAAATTTGGAAACTACCTGAAGGTA
CTCTAAATGGTAAGCCTGGAGCTCATGCCGTTGTTCAAGACCGTATGCTAAAAGATGGCAAGATCAATGCTTACTGGGTT
ATGTGTAATAACAACATGCAAGCTGGTCCAAATATTAACACTGAACGTCTACCAGGTTATCGTAATCCTGAAAACTTCAT
TGTATGTTCAGACCCATACCCAACAGCAACTGCACAAGCGGCTGACTTGATTCTACCAACCGCAATGTGGGTAGAAAAAG
AAGGTGCTTATGGTAACGCAGAGCGTCGTACTCAAGCTTGGTATCAACAAGTACAAGCAAAAGGCGAAGCAAAATCAGAT
TTATGGCAAATCATGGAGTTCTCAAAACGCTTCAAAGTTGAAGAAGTGTGGGGCGAAGAGCTAGTAGCAAAAGCGCCTGA
ATACCGTGGTAAAACAATGTATGACATTTTGTTCAAAAATGGTCAAGTTGATGCCTTCCCATTATCAGAAGCGCAAGAGC
TTAATGATGATGCTAAAGCTCAAGGCTTCTATGTTCAAAAAGGTCTGTTTGAAGAATATGCAAGCTTCGGTCGTGGCCAT
GGACACGATTTAGCACCGTACGATACCTATCACACAGTGCGTGGTTTACGCTGGCCTGTTGTTGATGGTAAAGAAACGCT
ATGGCGCTTTAAAGAAGGTTCAGATCCATATGCGAAGAAAGGATCAGACTGGGATTTCTACGGTAAACCTGATGGTAAGG
CCTTAATTATCTCTGCACCATATGAAGCGCCACCTGAAGTGCCAAATGATGAGTTTGATATGTGGTTATGTACTGGTCGT
GTTCTTGAACACTGGCATACAGGTACTATGACTCGTCGTGTTCCTGAGCTGTATAAAGCAGTTCCTGATGCGCTTTGTTA
TATCCACCCAGCAGATGCGAAAAAACGTAATTTACGTCGTGGAGATGAAGTTCTAATCTCAAACAAACGTGGTGAAGTAC
GAGTTCGTGTTGAAACTCGTGGCCGTAACCGTCCGCCAGAAGGATTGGTATTCGTACCATTCTTTGATGCTCGCATCTTA
ATTAACAAGTTGATTCTAGACGCAACTGATCCGCTATCTAAGCAGACAGACTTTAAGAAGTGTCCAGTTAAAATCACTAA
AATTGCTTAA

Upstream 100 bases:

>100_bases
CCGATTCAATCGATAAAATAAATGACTTCGAAGACGTACTAAATGTCGCTTTAGTATTTCACCAAATCGAGCACTTTGAT
TCGCAATGTGAGGATGAATC

Downstream 100 bases:

>100_bases
TTAATAAATTGATGAGTTGCTAGTCTACTGGCTAGTGACTTGTTTTAGAGTTTGCCTTAAGGCGGAGAAATTAGAATGAA
AAAGTTCATTATAGCGCTTA

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MKMTRRAFVKANAAASAAAVAGVTLPASATNLIVSSDQTKIKWDKAPCRFCGTGCSVLVGTQNGRVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGKDRVQTPMLRMKDGQYNKDGDFAPVSWDVALDTMAEKWKAALKKHGPTGVGMFGSGQWTVMEGYA
AVKLMKAGFRSNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDFENADSFVLWGSNMAEMHPVLWTRISDRRLSHPHVK
VNVLSTYYHRSFELADKGYIFEPQSDLAIANFIANYIIQNNAVNWDFVNKHTNFKQATTDIGYGLRDDDPLQMEAANPNS
GAMSSISFEEYKKSVAPYTAQKASEMSGVSEEDLITLAKQYADPKTKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHRAISEKIWKLPEGTLNGKPGAHAVVQDRMLKDGKINAYWV
MCNNNMQAGPNINTERLPGYRNPENFIVCSDPYPTATAQAADLILPTAMWVEKEGAYGNAERRTQAWYQQVQAKGEAKSD
LWQIMEFSKRFKVEEVWGEELVAKAPEYRGKTMYDILFKNGQVDAFPLSEAQELNDDAKAQGFYVQKGLFEEYASFGRGH
GHDLAPYDTYHTVRGLRWPVVDGKETLWRFKEGSDPYAKKGSDWDFYGKPDGKALIISAPYEAPPEVPNDEFDMWLCTGR
VLEHWHTGTMTRRVPELYKAVPDALCYIHPADAKKRNLRRGDEVLISNKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA

Sequences:

>Translated_829_residues
MKMTRRAFVKANAAASAAAVAGVTLPASATNLIVSSDQTKIKWDKAPCRFCGTGCSVLVGTQNGRVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGKDRVQTPMLRMKDGQYNKDGDFAPVSWDVALDTMAEKWKAALKKHGPTGVGMFGSGQWTVMEGYA
AVKLMKAGFRSNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDFENADSFVLWGSNMAEMHPVLWTRISDRRLSHPHVK
VNVLSTYYHRSFELADKGYIFEPQSDLAIANFIANYIIQNNAVNWDFVNKHTNFKQATTDIGYGLRDDDPLQMEAANPNS
GAMSSISFEEYKKSVAPYTAQKASEMSGVSEEDLITLAKQYADPKTKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHRAISEKIWKLPEGTLNGKPGAHAVVQDRMLKDGKINAYWV
MCNNNMQAGPNINTERLPGYRNPENFIVCSDPYPTATAQAADLILPTAMWVEKEGAYGNAERRTQAWYQQVQAKGEAKSD
LWQIMEFSKRFKVEEVWGEELVAKAPEYRGKTMYDILFKNGQVDAFPLSEAQELNDDAKAQGFYVQKGLFEEYASFGRGH
GHDLAPYDTYHTVRGLRWPVVDGKETLWRFKEGSDPYAKKGSDWDFYGKPDGKALIISAPYEAPPEVPNDEFDMWLCTGR
VLEHWHTGTMTRRVPELYKAVPDALCYIHPADAKKRNLRRGDEVLISNKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA
>Mature_829_residues
MKMTRRAFVKANAAASAAAVAGVTLPASATNLIVSSDQTKIKWDKAPCRFCGTGCSVLVGTQNGRVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGKDRVQTPMLRMKDGQYNKDGDFAPVSWDVALDTMAEKWKAALKKHGPTGVGMFGSGQWTVMEGYA
AVKLMKAGFRSNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDFENADSFVLWGSNMAEMHPVLWTRISDRRLSHPHVK
VNVLSTYYHRSFELADKGYIFEPQSDLAIANFIANYIIQNNAVNWDFVNKHTNFKQATTDIGYGLRDDDPLQMEAANPNS
GAMSSISFEEYKKSVAPYTAQKASEMSGVSEEDLITLAKQYADPKTKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHRAISEKIWKLPEGTLNGKPGAHAVVQDRMLKDGKINAYWV
MCNNNMQAGPNINTERLPGYRNPENFIVCSDPYPTATAQAADLILPTAMWVEKEGAYGNAERRTQAWYQQVQAKGEAKSD
LWQIMEFSKRFKVEEVWGEELVAKAPEYRGKTMYDILFKNGQVDAFPLSEAQELNDDAKAQGFYVQKGLFEEYASFGRGH
GHDLAPYDTYHTVRGLRWPVVDGKETLWRFKEGSDPYAKKGSDWDFYGKPDGKALIISAPYEAPPEVPNDEFDMWLCTGR
VLEHWHTGTMTRRVPELYKAVPDALCYIHPADAKKRNLRRGDEVLISNKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=69.1566265060241, Blast_Score=1228, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=801, Percent_Identity=24.0948813982522, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1787870, Length=835, Percent_Identity=23.8323353293413, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI3868720, Length=661, Percent_Identity=21.9364599092284, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI3868719, Length=660, Percent_Identity=20.9090909090909, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_VIBF1 (Q5E3J6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205288.1
- ProteinModelPortal:   Q5E3J6
- SMR:   Q5E3J6
- STRING:   Q5E3J6
- GeneID:   3279209
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1905
- NMPDR:   fig|312309.3.peg.1905
- eggNOG:   COG0243
- HOGENOM:   HBG715033
- OMA:   IISAPYE
- PhylomeDB:   Q5E3J6
- ProtClustDB:   PRK13532
- BioCyc:   MetaCyc:MONOMER-13447
- BioCyc:   VFIS312309:VF1905-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 92595; Mature: 92595

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKMTRRAFVKANAAASAAAVAGVTLPASATNLIVSSDQTKIKWDKAPCRFCGTGCSVLVG
CCCCHHHHHCCCCHHHHHHHHCEECCCCCCEEEEECCCCEEEECCCCCCEECCCCEEEEE
TQNGRVVATQGDPEAPVNKGLNCIKGYFLSKIMYGKDRVQTPMLRMKDGQYNKDGDFAPV
CCCCEEEEECCCCCCCHHHCHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCCCCE
SWDVALDTMAEKWKAALKKHGPTGVGMFGSGQWTVMEGYAAVKLMKAGFRSNNIDPNARH
EEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVGAFMRTFGIDEPMGCYDDFENADSFVLWGSNMAEMHPVLWTRISDRRLSHPHVK
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHHHHCHHHHEEECCCCCCCCCEE
VNVLSTYYHRSFELADKGYIFEPQSDLAIANFIANYIIQNNAVNWDFVNKHTNFKQATTD
EEEEEHHHHCEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
IGYGLRDDDPLQMEAANPNSGAMSSISFEEYKKSVAPYTAQKASEMSGVSEEDLITLAKQ
HCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
YADPKTKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKIATPGNSPFSLTGQPSACGTAR
HCCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEEEEEEEEECCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADMVVANPKHRAISEKIWKLPEGTLNGKPGAHAVVQDRMLKDGKINAYWV
HHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
MCNNNMQAGPNINTERLPGYRNPENFIVCSDPYPTATAQAADLILPTAMWVEKEGAYGNA
EECCCCCCCCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHEEECCCCCCCH
ERRTQAWYQQVQAKGEAKSDLWQIMEFSKRFKVEEVWGEELVAKAPEYRGKTMYDILFKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECC
GQVDAFPLSEAQELNDDAKAQGFYVQKGLFEEYASFGRGHGHDLAPYDTYHTVRGLRWPV
CCEEEECCCHHHHCCCCHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
VDGKETLWRFKEGSDPYAKKGSDWDFYGKPDGKALIISAPYEAPPEVPNDEFDMWLCTGR
CCCHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEHHH
VLEHWHTGTMTRRVPELYKAVPDALCYIHPADAKKRNLRRGDEVLISNKRGEVRVRVETR
HHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCEEEEECCCCCEEEEEEEC
GRNRPPEGLVFVPFFDARILINKLILDATDPLSKQTDFKKCPVKITKIA
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKMTRRAFVKANAAASAAAVAGVTLPASATNLIVSSDQTKIKWDKAPCRFCGTGCSVLVG
CCCCHHHHHCCCCHHHHHHHHCEECCCCCCEEEEECCCCEEEECCCCCCEECCCCEEEEE
TQNGRVVATQGDPEAPVNKGLNCIKGYFLSKIMYGKDRVQTPMLRMKDGQYNKDGDFAPV
CCCCEEEEECCCCCCCHHHCHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCCCCE
SWDVALDTMAEKWKAALKKHGPTGVGMFGSGQWTVMEGYAAVKLMKAGFRSNNIDPNARH
EEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVGAFMRTFGIDEPMGCYDDFENADSFVLWGSNMAEMHPVLWTRISDRRLSHPHVK
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHHHHCHHHHEEECCCCCCCCCEE
VNVLSTYYHRSFELADKGYIFEPQSDLAIANFIANYIIQNNAVNWDFVNKHTNFKQATTD
EEEEEHHHHCEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
IGYGLRDDDPLQMEAANPNSGAMSSISFEEYKKSVAPYTAQKASEMSGVSEEDLITLAKQ
HCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
YADPKTKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKIATPGNSPFSLTGQPSACGTAR
HCCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEEEEEEEEECCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADMVVANPKHRAISEKIWKLPEGTLNGKPGAHAVVQDRMLKDGKINAYWV
HHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
MCNNNMQAGPNINTERLPGYRNPENFIVCSDPYPTATAQAADLILPTAMWVEKEGAYGNA
EECCCCCCCCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHEEECCCCCCCH
ERRTQAWYQQVQAKGEAKSDLWQIMEFSKRFKVEEVWGEELVAKAPEYRGKTMYDILFKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECC
GQVDAFPLSEAQELNDDAKAQGFYVQKGLFEEYASFGRGHGHDLAPYDTYHTVRGLRWPV
CCEEEECCCHHHHCCCCHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
VDGKETLWRFKEGSDPYAKKGSDWDFYGKPDGKALIISAPYEAPPEVPNDEFDMWLCTGR
CCCHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEHHH
VLEHWHTGTMTRRVPELYKAVPDALCYIHPADAKKRNLRRGDEVLISNKRGEVRVRVETR
HHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCEEEEECCCCCEEEEEEEC
GRNRPPEGLVFVPFFDARILINKLILDATDPLSKQTDFKKCPVKITKIA
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA