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The map label for this gene is napB [H]

Identifier: 59712511

GI number: 59712511

Start: 2136139

End: 2136612

Strand: Reverse

Name: napB [H]

Synonym: VF_1904

Alternate gene names: 59712511

Gene position: 2136612-2136139 (Counterclockwise)

Preceding gene: 59712512

Following gene: 59712510

Centisome position: 73.74

GC content: 43.04

Gene sequence:

>474_bases
ATGAAAAAGTTCATTATAGCGCTTATGTCAGCAGGACTATTGTTCACTGGCGCAGTACAAGCAGAAGCAGAACAAGGTTC
TGAACTGCATAACCCAGGTGGTATTGGTGGTGTTGAGTCATTACGTGGTGCTTCAGAGTTAGAAGCGACACGTCCAGCGG
ATGATTTTAAGCGTTTTCCACGTGACCACGCTTTAGATAGTGACTATGTATATCAACCACCTTTGGTTCCTCATACGATT
CGTAACTATGAAGTATCGTTGAATGCCAATAAGTGTTTGTCTTGTCATAGTTGGAAAAATGCAAAAGAAATGGGGGCAAC
TAAGATTAGTGTGACTCATTACGTAAACCGTGAAGATGCAGTGCTTTCTGATGTATCTCCTCGTCGTTACTTCTGTCTGC
AATGTCACGTTCCTCAAGCGGATGCAAAACCACTAGTAGAGAACGAATTTGAACGTGTGGACTCATTGCGCTAG

Upstream 100 bases:

>100_bases
AGTTAAAATCACTAAAATTGCTTAATTAATAAATTGATGAGTTGCTAGTCTACTGGCTAGTGACTTGTTTTAGAGTTTGC
CTTAAGGCGGAGAAATTAGA

Downstream 100 bases:

>100_bases
TCGATAGCCAATAAAGTTAAGAGAGGCTATACATGAAATTATTAAAAGCATTTTGGAAGCGTTTTTCGTCTCCTAGTAAA
GCTGCAGTAGGCATTGTTCT

Product: nitrate reductase, small, cytochrome C550 subunit, periplasmic

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKKFIIALMSAGLLFTGAVQAEAEQGSELHNPGGIGGVESLRGASELEATRPADDFKRFPRDHALDSDYVYQPPLVPHTI
RNYEVSLNANKCLSCHSWKNAKEMGATKISVTHYVNREDAVLSDVSPRRYFCLQCHVPQADAKPLVENEFERVDSLR

Sequences:

>Translated_157_residues
MKKFIIALMSAGLLFTGAVQAEAEQGSELHNPGGIGGVESLRGASELEATRPADDFKRFPRDHALDSDYVYQPPLVPHTI
RNYEVSLNANKCLSCHSWKNAKEMGATKISVTHYVNREDAVLSDVSPRRYFCLQCHVPQADAKPLVENEFERVDSLR
>Mature_157_residues
MKKFIIALMSAGLLFTGAVQAEAEQGSELHNPGGIGGVESLRGASELEATRPADDFKRFPRDHALDSDYVYQPPLVPHTI
RNYEVSLNANKCLSCHSWKNAKEMGATKISVTHYVNREDAVLSDVSPRRYFCLQCHVPQADAKPLVENEFERVDSLR

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=94, Percent_Identity=46.8085106382979, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 17488; Mature: 17488

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIIALMSAGLLFTGAVQAEAEQGSELHNPGGIGGVESLRGASELEATRPADDFKRFP
CHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHCCCCCHHHHHHCC
RDHALDSDYVYQPPLVPHTIRNYEVSLNANKCLSCHSWKNAKEMGATKISVTHYVNREDA
CCCCCCCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCEEEEEEEECCCCCH
VLSDVSPRRYFCLQCHVPQADAKPLVENEFERVDSLR
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFIIALMSAGLLFTGAVQAEAEQGSELHNPGGIGGVESLRGASELEATRPADDFKRFP
CHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHCCCCCHHHHHHCC
RDHALDSDYVYQPPLVPHTIRNYEVSLNANKCLSCHSWKNAKEMGATKISVTHYVNREDA
CCCCCCCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCEEEEEEEECCCCCH
VLSDVSPRRYFCLQCHVPQADAKPLVENEFERVDSLR
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 11389694; 11223519 [H]