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The map label for this gene is ligA [H]

Identifier: 59712497

GI number: 59712497

Start: 2124578

End: 2126584

Strand: Reverse

Name: ligA [H]

Synonym: VF_1890

Alternate gene names: 59712497

Gene position: 2126584-2124578 (Counterclockwise)

Preceding gene: 59712498

Following gene: 59712496

Centisome position: 73.39

GC content: 40.91

Gene sequence:

>2007_bases
ATGAGTATTGAACAGACATTAGATGAGTTGAAACAACAACTGAATTACCATGCATATCGTTATTATGTAGAGGATAGCCC
TGAGCTACCTGATGCAGAATATGATCGAATGATGCAGCAGTTATTATCTATTGAATCTGAACATCCAGAATTAGTTACTG
TTGATTCACCTAGTCAACGAGTAGGGGGAGAGGCGCTAGATGGTTTTACTCAGATCCAACATGAGATCCCTATGCTTTCT
TTGGATAATGCGTTTAGTGATGAAGAGCTAGAAGCATTTGAAAAAAGAATGAACGACCGTCTTATCTCAAAACCTGTATC
TCTATTTTGTTGTGAACCTAAGTTGGACGGCTTAGCGGTTAGTCTTTTATATGTAGATGGCAAGTTAGTTCAAGCAGGTA
CGCGTGGAGATGGTACAACAGGTGAAAATATTACAGAAAACGTAAGAACGATTCGTTGTATCCCATTAACATTACAAGGT
GAAGGCTGGCCGACACGTTTAGAAGTGCGTGGTGAAGTCTTTATGCCTAAAGCCGGTTTTGAAGCGTTAAATGAGAGAGC
TTTAAAACGTGGTGAAAAACCATTTGCAAATCCACGTAATGCTGCAGCAGGCAGTTTACGTCAATTAGATTCAAAAATAA
CAGCAACCCGCCCACTTAGTTTTTATGCATATAGTGTTGGGGTGATTGAAGGTGGTGAATTAGAAACAAGCCATTACCAG
CGTTTTGTTCAATTAAAAAGCTGGGGTCTTCCTATGTGCGAAGAAACTAAGCAATGTCATTCATTGACAGAAGTTAAAGC
GTATTACAAAGACATACTTGAGCGTCGAGATGCTCTGAAATATGAAATTGATGGTGTAGTTATAAAAGTCGATGACATTG
CTCTTCAAGAACAATTAGGTTTTGTTGCACGAGCTCCTCGTTGGGCGATTGCTTATAAATTTCCTGCACAAGAAGAGTTA
ACCGTTCTAAATGATGTGGAATTCCAAGTTGGCCGTACAGGCGCAATTACACCGGTAGCGAAACTTGAACCCGTTTTTGT
TGGTGGTGTAACCGTAAGTAATGCCACATTGCATAATGCAGATGAAATCGCACGTTTAGGTGTTCATATTGGTGATACGG
TGATTATTCGCCGTGCAGGGGACGTGATCCCGCAAATCGTATCAGTAGTAGAAGCCCGTCGCCCTACAGACAGTAAAGCT
ATTGTTTACCCAATCCATTGCCCTGTATGTGGCTCTAACCTTGAAAGAGTAGAAGGTGAGGCCGTTACTCGTTGTGGAGC
AGGGTTAGTCTGTCAAGCACAACGCAAAGAAGCACTAAAGCACTTTGTGTCTCGTAAAGCACTCGATGTTGATGGTTTAG
GCGATAAAGTTGTAGAACAGCTTGTAGATAAAGAAATGGTAGAGACTCCTGCGGATTTATTTAAACTTTCTGCGGGTCTA
TTGACAGTATTAGAGCGCATGGGACCTAAATCTGCGCAAAATGTAGTGGATGCGCTTAATAAAGCAAAGGCGACGACATT
ACCGCGTTTTCTATACTCGTTAGGTATTCGAGAAGTTGGTGAGGCGACAGCTGCAAACTTAGCCGCACATTTTTATACAT
TAGAAGCGATTCAAGTAGCGACATTTGAGCAACTTATTGAAGTGAGTGATGTTGGTGATATTGTCGCTAAACACGTTTTA
AACTTCTTTGCAGAGCCGCATAACAAAACTGTTATTGAAGATTTACAAGAGATGGGTATCCATTGGCCTGAGATTAAAGC
GCTTGATGAATCAGTACCACAACCATTAGCGGGAAAAGTAGTGGTGCTTACTGGTACGCTTCATAAATTAAAACGTAATG
AAGCTAAAGCAGCTTTACAAGAGTTAGGCGCAAAGGTAACAGGCAGTGTTTCTAAGAAAACAGATATTCTATTTGCTGGT
GAAGCTGCCGGGTCTAAATTAGCGAAAGCCGAAGAACTTGGGGTTGAAGTTATGAATGAAGAGCAGTTAATTGAATTATT
AAATTAA

Upstream 100 bases:

>100_bases
CGTAAATAGAATATCTACTATAGAATTAGGATTAATGAGAAAGGCTCATCATGAGCCTTTTTTTTATTTTAAGAACAACC
GAATAAGAAGTTGATGAATC

Downstream 100 bases:

>100_bases
GACTAATAATTTCGGTTATTAAAAAGGAGCCTTATATGGCTCCTTTTTTTATTGGTAAAAATATTAAATTATTTTTGGTT
TGTTTAAAGTGAATTATTAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSIEQTLDELKQQLNYHAYRYYVEDSPELPDAEYDRMMQQLLSIESEHPELVTVDSPSQRVGGEALDGFTQIQHEIPMLS
LDNAFSDEELEAFEKRMNDRLISKPVSLFCCEPKLDGLAVSLLYVDGKLVQAGTRGDGTTGENITENVRTIRCIPLTLQG
EGWPTRLEVRGEVFMPKAGFEALNERALKRGEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGVIEGGELETSHYQ
RFVQLKSWGLPMCEETKQCHSLTEVKAYYKDILERRDALKYEIDGVVIKVDDIALQEQLGFVARAPRWAIAYKFPAQEEL
TVLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPTDSKA
IVYPIHCPVCGSNLERVEGEAVTRCGAGLVCQAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGL
LTVLERMGPKSAQNVVDALNKAKATTLPRFLYSLGIREVGEATAANLAAHFYTLEAIQVATFEQLIEVSDVGDIVAKHVL
NFFAEPHNKTVIEDLQEMGIHWPEIKALDESVPQPLAGKVVVLTGTLHKLKRNEAKAALQELGAKVTGSVSKKTDILFAG
EAAGSKLAKAEELGVEVMNEEQLIELLN

Sequences:

>Translated_668_residues
MSIEQTLDELKQQLNYHAYRYYVEDSPELPDAEYDRMMQQLLSIESEHPELVTVDSPSQRVGGEALDGFTQIQHEIPMLS
LDNAFSDEELEAFEKRMNDRLISKPVSLFCCEPKLDGLAVSLLYVDGKLVQAGTRGDGTTGENITENVRTIRCIPLTLQG
EGWPTRLEVRGEVFMPKAGFEALNERALKRGEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGVIEGGELETSHYQ
RFVQLKSWGLPMCEETKQCHSLTEVKAYYKDILERRDALKYEIDGVVIKVDDIALQEQLGFVARAPRWAIAYKFPAQEEL
TVLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPTDSKA
IVYPIHCPVCGSNLERVEGEAVTRCGAGLVCQAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGL
LTVLERMGPKSAQNVVDALNKAKATTLPRFLYSLGIREVGEATAANLAAHFYTLEAIQVATFEQLIEVSDVGDIVAKHVL
NFFAEPHNKTVIEDLQEMGIHWPEIKALDESVPQPLAGKVVVLTGTLHKLKRNEAKAALQELGAKVTGSVSKKTDILFAG
EAAGSKLAKAEELGVEVMNEEQLIELLN
>Mature_667_residues
SIEQTLDELKQQLNYHAYRYYVEDSPELPDAEYDRMMQQLLSIESEHPELVTVDSPSQRVGGEALDGFTQIQHEIPMLSL
DNAFSDEELEAFEKRMNDRLISKPVSLFCCEPKLDGLAVSLLYVDGKLVQAGTRGDGTTGENITENVRTIRCIPLTLQGE
GWPTRLEVRGEVFMPKAGFEALNERALKRGEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGVIEGGELETSHYQR
FVQLKSWGLPMCEETKQCHSLTEVKAYYKDILERRDALKYEIDGVVIKVDDIALQEQLGFVARAPRWAIAYKFPAQEELT
VLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPTDSKAI
VYPIHCPVCGSNLERVEGEAVTRCGAGLVCQAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGLL
TVLERMGPKSAQNVVDALNKAKATTLPRFLYSLGIREVGEATAANLAAHFYTLEAIQVATFEQLIEVSDVGDIVAKHVLN
FFAEPHNKTVIEDLQEMGIHWPEIKALDESVPQPLAGKVVVLTGTLHKLKRNEAKAALQELGAKVTGSVSKKTDILFAGE
AAGSKLAKAEELGVEVMNEEQLIELLN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=65.2173913043478, Blast_Score=903, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=519, Percent_Identity=21.9653179190751, Blast_Score=102, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73611; Mature: 73480

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEQTLDELKQQLNYHAYRYYVEDSPELPDAEYDRMMQQLLSIESEHPELVTVDSPSQR
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHH
VGGEALDGFTQIQHEIPMLSLDNAFSDEELEAFEKRMNDRLISKPVSLFCCEPKLDGLAV
CCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
SLLYVDGKLVQAGTRGDGTTGENITENVRTIRCIPLTLQGEGWPTRLEVRGEVFMPKAGF
EEEEECCEEEECCCCCCCCCCCCHHHCCEEEEEEEEEEECCCCCEEEEECCCEECCCHHH
EALNERALKRGEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGVIEGGELETSHYQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHH
RFVQLKSWGLPMCEETKQCHSLTEVKAYYKDILERRDALKYEIDGVVIKVDDIALQEQLG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEECCHHHHHHHH
FVARAPRWAIAYKFPAQEELTVLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNA
HHHCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCEEEECCEEECCCCCCCH
DEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPTDSKAIVYPIHCPVCGSNLERVEGE
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCH
AVTRCGAGLVCQAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGL
HHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
LTVLERMGPKSAQNVVDALNKAKATTLPRFLYSLGIREVGEATAANLAAHFYTLEAIQVA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFEQLIEVSDVGDIVAKHVLNFFAEPHNKTVIEDLQEMGIHWPEIKALDESVPQPLAGKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEE
VVLTGTLHKLKRNEAKAALQELGAKVTGSVSKKTDILFAGEAAGSKLAKAEELGVEVMNE
EEEECHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHCCH
EQLIELLN
HHHHHHCC
>Mature Secondary Structure 
SIEQTLDELKQQLNYHAYRYYVEDSPELPDAEYDRMMQQLLSIESEHPELVTVDSPSQR
CHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHH
VGGEALDGFTQIQHEIPMLSLDNAFSDEELEAFEKRMNDRLISKPVSLFCCEPKLDGLAV
CCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
SLLYVDGKLVQAGTRGDGTTGENITENVRTIRCIPLTLQGEGWPTRLEVRGEVFMPKAGF
EEEEECCEEEECCCCCCCCCCCCHHHCCEEEEEEEEEEECCCCCEEEEECCCEECCCHHH
EALNERALKRGEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGVIEGGELETSHYQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHH
RFVQLKSWGLPMCEETKQCHSLTEVKAYYKDILERRDALKYEIDGVVIKVDDIALQEQLG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEECCHHHHHHHH
FVARAPRWAIAYKFPAQEELTVLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNA
HHHCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCEEEECCEEECCCCCCCH
DEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPTDSKAIVYPIHCPVCGSNLERVEGE
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCH
AVTRCGAGLVCQAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGL
HHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
LTVLERMGPKSAQNVVDALNKAKATTLPRFLYSLGIREVGEATAANLAAHFYTLEAIQVA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFEQLIEVSDVGDIVAKHVLNFFAEPHNKTVIEDLQEMGIHWPEIKALDESVPQPLAGKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEE
VVLTGTLHKLKRNEAKAALQELGAKVTGSVSKKTDILFAGEAAGSKLAKAEELGVEVMNE
EEEECHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHCCH
EQLIELLN
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA