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The map label for this gene is yfbB [H]

Identifier: 59712277

GI number: 59712277

Start: 1879064

End: 1879858

Strand: Reverse

Name: yfbB [H]

Synonym: VF_1670

Alternate gene names: 59712277

Gene position: 1879858-1879064 (Counterclockwise)

Preceding gene: 59712278

Following gene: 172087717

Centisome position: 64.88

GC content: 39.25

Gene sequence:

>795_bases
ATGCCGCTTTATTCTAATCAATTGGAAACCATGAAAAATACCACTCAACCTTGCGTGGTATTTTTACATGGATTGTTAGG
ATCAACGAAAGACTGGTCAAGCATTGCTGCAAAGGTTGCAAAGACGCATCCGGTATTATTAATTGATCTTCCAGGACATG
GAAACAGTCAATCAACCTTATTGGATCACAATGAGGGATTTGAACAGAGCTGCCAGTTGATTGTTGAACAATTGGAAAAA
TCACCTTATCAGACCTTTATCCTTGTTGGATACTCGTTGGGTGGGCGATTAGCGATGTATCTTCATGCTATGTATTCTTT
GCCTTCTTCGATAGAGGTGAAAGGACTGTGTATTGAAGGTGGAAACTTTGGCTTAGTTTCAGAAGAAGAAAAGCTGCTAC
GCTTGGAAAATGATATTCAATGGGCAAAGCGCTTTGAAGAACTACCTATTGTTGATGTTTTAGACAATTGGTATCAACAA
GCGGTGTTTTCTTCACTAAACCCTGAGCAAAGACAAGTTTTAGTAACCAAGAGAAGTGATAATCTTGGACCTGCAATTGG
AATGATGTTGAGATCTACGTCACTTGCTAAGCAACCATATTTGCTTGATGCATTACATCGTAGCACCATTCCAATTCTTT
ATATTTGCGGCGAAGCAGATAAAAAATTTCAACACTTGGCTGAGCAAAGTCAATTAACGTATCAAATCATTGCTCAGGCA
GGGCATAACGCTCATGTGGAACAGCCCGAACGCTTTACACATGTACTTAATACATTCTTACAACAATGGACATAA

Upstream 100 bases:

>100_bases
ATTAACGATGGCTTAGAAAAGGGTATTCATTTAATTGAGATCAATACACCAGCAGGTCAATCTGGTGAAGAGCTAACACG
TCTTTTTCAGACCATTCAGC

Downstream 100 bases:

>100_bases
ATCCCATGGCAAGAACTGTTGGCATTTCAGAAGAAGAACTTTACGCTCCAGTAGATTGGACAGATTGCACTGCGCAATAC
GAAGACATTCAGTACCACAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPLYSNQLETMKNTTQPCVVFLHGLLGSTKDWSSIAAKVAKTHPVLLIDLPGHGNSQSTLLDHNEGFEQSCQLIVEQLEK
SPYQTFILVGYSLGGRLAMYLHAMYSLPSSIEVKGLCIEGGNFGLVSEEEKLLRLENDIQWAKRFEELPIVDVLDNWYQQ
AVFSSLNPEQRQVLVTKRSDNLGPAIGMMLRSTSLAKQPYLLDALHRSTIPILYICGEADKKFQHLAEQSQLTYQIIAQA
GHNAHVEQPERFTHVLNTFLQQWT

Sequences:

>Translated_264_residues
MPLYSNQLETMKNTTQPCVVFLHGLLGSTKDWSSIAAKVAKTHPVLLIDLPGHGNSQSTLLDHNEGFEQSCQLIVEQLEK
SPYQTFILVGYSLGGRLAMYLHAMYSLPSSIEVKGLCIEGGNFGLVSEEEKLLRLENDIQWAKRFEELPIVDVLDNWYQQ
AVFSSLNPEQRQVLVTKRSDNLGPAIGMMLRSTSLAKQPYLLDALHRSTIPILYICGEADKKFQHLAEQSQLTYQIIAQA
GHNAHVEQPERFTHVLNTFLQQWT
>Mature_263_residues
PLYSNQLETMKNTTQPCVVFLHGLLGSTKDWSSIAAKVAKTHPVLLIDLPGHGNSQSTLLDHNEGFEQSCQLIVEQLEKS
PYQTFILVGYSLGGRLAMYLHAMYSLPSSIEVKGLCIEGGNFGLVSEEEKLLRLENDIQWAKRFEELPIVDVLDNWYQQA
VFSSLNPEQRQVLVTKRSDNLGPAIGMMLRSTSLAKQPYLLDALHRSTIPILYICGEADKKFQHLAEQSQLTYQIIAQAG
HNAHVEQPERFTHVLNTFLQQWT

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=233, Percent_Identity=41.2017167381974, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 29780; Mature: 29649

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYSNQLETMKNTTQPCVVFLHGLLGSTKDWSSIAAKVAKTHPVLLIDLPGHGNSQSTL
CCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEE
LDHNEGFEQSCQLIVEQLEKSPYQTFILVGYSLGGRLAMYLHAMYSLPSSIEVKGLCIEG
ECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEC
GNFGLVSEEEKLLRLENDIQWAKRFEELPIVDVLDNWYQQAVFSSLNPEQRQVLVTKRSD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCC
NLGPAIGMMLRSTSLAKQPYLLDALHRSTIPILYICGEADKKFQHLAEQSQLTYQIIAQA
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GHNAHVEQPERFTHVLNTFLQQWT
CCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLYSNQLETMKNTTQPCVVFLHGLLGSTKDWSSIAAKVAKTHPVLLIDLPGHGNSQSTL
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEE
LDHNEGFEQSCQLIVEQLEKSPYQTFILVGYSLGGRLAMYLHAMYSLPSSIEVKGLCIEG
ECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEC
GNFGLVSEEEKLLRLENDIQWAKRFEELPIVDVLDNWYQQAVFSSLNPEQRQVLVTKRSD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCC
NLGPAIGMMLRSTSLAKQPYLLDALHRSTIPILYICGEADKKFQHLAEQSQLTYQIIAQA
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GHNAHVEQPERFTHVLNTFLQQWT
CCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA