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The map label for this gene is topB [H]

Identifier: 59712248

GI number: 59712248

Start: 1844249

End: 1846219

Strand: Reverse

Name: topB [H]

Synonym: VF_1641

Alternate gene names: 59712248

Gene position: 1846219-1844249 (Counterclockwise)

Preceding gene: 59712249

Following gene: 59712247

Centisome position: 63.72

GC content: 40.84

Gene sequence:

>1971_bases
ATGTTATTCACAACATCAGCATTTTTTATGTCACGATTATTTATTGCCGAAAAACCAAGCCTTGGCAGAGCGATTGCCGC
AGGCCTAGCAAAACCACAAAAAAACCAACAAGGTTACATCGAATGCGGCAATGGAGATATTGTGACATGGTGTATTGGTC
ACCTATTAGAGCAAGTTGAACCCGATGCATATGATGATAAATATAAATCATGGCGTATGGAGCATTTACCTATCGTGCCA
GATCAGTGGCAACTTAGACCAAGAAAAGCCTCCAGTAAGCAATTAACGGTAATTCGAAAGTTGTTAAAAAATGCAACGCA
GATCGTTCATGCGGGAGACCCTGATAGAGAAGGTCAGTTACTGGTGGATGAGGTGTTGGATTATTTAAAATTATCTAAAA
CGAAAAAAGCGGCGACTCAACGCTTATTGATCAGTGATTTAAACCTGCCAGCGGTGAAGCGTGCGTTGACTTCTATGCGA
AGTAATCAAGAGTTTATTCCTCTCTCTATTTCGGCCTTAGCACGCTCTCGTGCCGATTGGTTATATGGTATGAATATGAC
ACGTGCTTACACGTTATTAGGTCAACGAGGTGGTTATCAAGGAGTATTATCAGTAGGAAGAGTACAAACTCCTGTTTTAG
GTTTGGTTACTCGCCGTGATGAAGAAATTGCTAATTTTGTACCAAAGGCGTATTACACGTTAGATGCTCTTATCCCTTAT
CAAAATGATCAATTAAACGACGGTGTTCCTTTTGATATCAGAGCAAAATGGAAGCCGAGTGATGCATGCCAGCCATGGCA
AGATGAAGAAGGACGAGTTCTTAATCTTAAACTGGTTGAAAATGTCGCTCAGCGCATAGCGAATCAACCGGCGACAGTAA
CTAAAGCTGAAAATAAAGAAACCAAACAAAATGCGCCTTTGCCATATTCTTTGTCATCGCTTCAAATCGATGCGGCAAAA
CGTTATGGACTAAGTGCACAGCAAGTGCTAGATGCGTGTCAGGCATTATATGAAAAGCATAAGTTAATAACCTATCCACG
TTCAGATAGTCGTCATTTACCAAAAGACCATTATAAACAGCGTGAGCAAGTGTGTGCTGCGATAGGAAATAATGATAAGC
AATTATCAACAGCTGTTACAAAAGCCGACTTTTCATTGAAATCGCGAGCTTGGAATGACAGTAAGGTGGATGCTCACCAT
GCTATTATTCCAACACCGAAATCCAGTAGTTCAGCTGGCTTATCTGGCTATGAAGAGAAAGTGTATCATTTGATTGCTCG
TCAATACCTAATGCAATTTTATCCTGCTGCGGTTTATGCTGAGGCTGAATTGGTGTTTGATATTGCTGGTGGGGTGTTTG
TTGCAAAAGGAAAGCAAATGCTGTTTGCTGGCTGGAGAGTTCTGCTTGGCAAAGTGCAATCTCAAAACAGTGATGATGAT
GTAGCGAGTAAAGTTCCGCCATTATCAAAAGGTGAGGTTCACACTTGTCGAGAAGGTGAAATAAAACATCGCATGACAGA
GCCGCCAAAACATTTTACTGAGGCGACGCTTTTGCAAGCAATGACAGGTATTGCACGCTTTGTGGAAGATAAAGAACTGA
AAAAAATACTAAGAGATACGGATGGTCTGGGAACCGAAGCGACTCGTGCGGGTATTTTAGATACATTATTTAAACGTCAG
TTATTAACTCGAAATGGAAAGTCCATTCATGCCAGTGAAGCAGGTAGAGGGCTTATTCATGCTTTACCAAATGAAGCAAC
TTACCCGGACATGACCGCTCATTGGGAATTTCAGCTTCAAGGTATGACAGAGAAAACACACGCTTATCGACCTTTTATGT
CGGAATTAGAAATAAAAGTGACTAATTTTATGCAAGGTGTTCAGCAAGGCCCAGTACCAGAGTCATTAAGAACATTAAAA
AGTCCAGCTAAACCACAATTTAAACGTAAAAGAAAAGCAAAGAAATCTTAA

Upstream 100 bases:

>100_bases
CAAAAAGAGCGATTCGTCAAGGAGTAGAGTTGGCTTCAAAGTTATAGCCTCTTAATAAACAAAAACTTGATAACTGTCTA
TTCATACAGGTATAGTGCTG

Downstream 100 bases:

>100_bases
AATACGATCCCCATCATCGATTCATTTTTAAGAATACACTATTGTGCCCTGATATTTATTATTTATGAAAAGGCGCAATA
TGTTTAAAGATGTAATCGGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MLFTTSAFFMSRLFIAEKPSLGRAIAAGLAKPQKNQQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVP
DQWQLRPRKASSKQLTVIRKLLKNATQIVHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALTSMR
SNQEFIPLSISALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIANFVPKAYYTLDALIPY
QNDQLNDGVPFDIRAKWKPSDACQPWQDEEGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSSLQIDAAK
RYGLSAQQVLDACQALYEKHKLITYPRSDSRHLPKDHYKQREQVCAAIGNNDKQLSTAVTKADFSLKSRAWNDSKVDAHH
AIIPTPKSSSSAGLSGYEEKVYHLIARQYLMQFYPAAVYAEAELVFDIAGGVFVAKGKQMLFAGWRVLLGKVQSQNSDDD
VASKVPPLSKGEVHTCREGEIKHRMTEPPKHFTEATLLQAMTGIARFVEDKELKKILRDTDGLGTEATRAGILDTLFKRQ
LLTRNGKSIHASEAGRGLIHALPNEATYPDMTAHWEFQLQGMTEKTHAYRPFMSELEIKVTNFMQGVQQGPVPESLRTLK
SPAKPQFKRKRKAKKS

Sequences:

>Translated_656_residues
MLFTTSAFFMSRLFIAEKPSLGRAIAAGLAKPQKNQQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVP
DQWQLRPRKASSKQLTVIRKLLKNATQIVHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALTSMR
SNQEFIPLSISALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIANFVPKAYYTLDALIPY
QNDQLNDGVPFDIRAKWKPSDACQPWQDEEGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSSLQIDAAK
RYGLSAQQVLDACQALYEKHKLITYPRSDSRHLPKDHYKQREQVCAAIGNNDKQLSTAVTKADFSLKSRAWNDSKVDAHH
AIIPTPKSSSSAGLSGYEEKVYHLIARQYLMQFYPAAVYAEAELVFDIAGGVFVAKGKQMLFAGWRVLLGKVQSQNSDDD
VASKVPPLSKGEVHTCREGEIKHRMTEPPKHFTEATLLQAMTGIARFVEDKELKKILRDTDGLGTEATRAGILDTLFKRQ
LLTRNGKSIHASEAGRGLIHALPNEATYPDMTAHWEFQLQGMTEKTHAYRPFMSELEIKVTNFMQGVQQGPVPESLRTLK
SPAKPQFKRKRKAKKS
>Mature_656_residues
MLFTTSAFFMSRLFIAEKPSLGRAIAAGLAKPQKNQQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVP
DQWQLRPRKASSKQLTVIRKLLKNATQIVHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALTSMR
SNQEFIPLSISALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIANFVPKAYYTLDALIPY
QNDQLNDGVPFDIRAKWKPSDACQPWQDEEGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSSLQIDAAK
RYGLSAQQVLDACQALYEKHKLITYPRSDSRHLPKDHYKQREQVCAAIGNNDKQLSTAVTKADFSLKSRAWNDSKVDAHH
AIIPTPKSSSSAGLSGYEEKVYHLIARQYLMQFYPAAVYAEAELVFDIAGGVFVAKGKQMLFAGWRVLLGKVQSQNSDDD
VASKVPPLSKGEVHTCREGEIKHRMTEPPKHFTEATLLQAMTGIARFVEDKELKKILRDTDGLGTEATRAGILDTLFKRQ
LLTRNGKSIHASEAGRGLIHALPNEATYPDMTAHWEFQLQGMTEKTHAYRPFMSELEIKVTNFMQGVQQGPVPESLRTLK
SPAKPQFKRKRKAKKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=634, Percent_Identity=22.5552050473186, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788061, Length=652, Percent_Identity=56.2883435582822, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=590, Percent_Identity=22.3728813559322, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=648, Percent_Identity=23.1481481481481, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=547, Percent_Identity=24.8628884826325, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73656; Mature: 73656

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTTSAFFMSRLFIAEKPSLGRAIAAGLAKPQKNQQGYIECGNGDIVTWCIGHLLEQVE
CEEECHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
PDAYDDKYKSWRMEHLPIVPDQWQLRPRKASSKQLTVIRKLLKNATQIVHAGDPDREGQL
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCC
LVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALTSMRSNQEFIPLSISALARSRADW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIANFVPKAYYTLDALIPY
HHCCCHHHHHHHHHCCCCCHHHHHHCCHHCCHHHHHHCCHHHHHHHCCHHHHHHHHCCCC
QNDQLNDGVPFDIRAKWKPSDACQPWQDEEGRVLNLKLVENVAQRIANQPATVTKAENKE
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCH
TKQNAPLPYSLSSLQIDAAKRYGLSAQQVLDACQALYEKHKLITYPRSDSRHLPKDHYKQ
HCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHH
REQVCAAIGNNDKQLSTAVTKADFSLKSRAWNDSKVDAHHAIIPTPKSSSSAGLSGYEEK
HHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHH
VYHLIARQYLMQFYPAAVYAEAELVFDIAGGVFVAKGKQMLFAGWRVLLGKVQSQNSDDD
HHHHHHHHHHHHHCCHHHHHHHEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCH
VASKVPPLSKGEVHTCREGEIKHRMTEPPKHFTEATLLQAMTGIARFVEDKELKKILRDT
HHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGLGTEATRAGILDTLFKRQLLTRNGKSIHASEAGRGLIHALPNEATYPDMTAHWEFQLQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCCCCCCCCEEEEEEEE
GMTEKTHAYRPFMSELEIKVTNFMQGVQQGPVPESLRTLKSPAKPQFKRKRKAKKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MLFTTSAFFMSRLFIAEKPSLGRAIAAGLAKPQKNQQGYIECGNGDIVTWCIGHLLEQVE
CEEECHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
PDAYDDKYKSWRMEHLPIVPDQWQLRPRKASSKQLTVIRKLLKNATQIVHAGDPDREGQL
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCC
LVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALTSMRSNQEFIPLSISALARSRADW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIANFVPKAYYTLDALIPY
HHCCCHHHHHHHHHCCCCCHHHHHHCCHHCCHHHHHHCCHHHHHHHCCHHHHHHHHCCCC
QNDQLNDGVPFDIRAKWKPSDACQPWQDEEGRVLNLKLVENVAQRIANQPATVTKAENKE
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCH
TKQNAPLPYSLSSLQIDAAKRYGLSAQQVLDACQALYEKHKLITYPRSDSRHLPKDHYKQ
HCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHH
REQVCAAIGNNDKQLSTAVTKADFSLKSRAWNDSKVDAHHAIIPTPKSSSSAGLSGYEEK
HHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHH
VYHLIARQYLMQFYPAAVYAEAELVFDIAGGVFVAKGKQMLFAGWRVLLGKVQSQNSDDD
HHHHHHHHHHHHHCCHHHHHHHEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCH
VASKVPPLSKGEVHTCREGEIKHRMTEPPKHFTEATLLQAMTGIARFVEDKELKKILRDT
HHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGLGTEATRAGILDTLFKRQLLTRNGKSIHASEAGRGLIHALPNEATYPDMTAHWEFQLQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCCCCCCCCEEEEEEEE
GMTEKTHAYRPFMSELEIKVTNFMQGVQQGPVPESLRTLKSPAKPQFKRKRKAKKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]