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The map label for this gene is pstA2 [H]

Identifier: 59712219

GI number: 59712219

Start: 1808493

End: 1809350

Strand: Direct

Name: pstA2 [H]

Synonym: VF_1612

Alternate gene names: 59712219

Gene position: 1808493-1809350 (Clockwise)

Preceding gene: 59712218

Following gene: 59712220

Centisome position: 62.41

GC content: 38.58

Gene sequence:

>858_bases
ATGAATAAATTAAAAAAACAGCGTCAAATAAAAGACCAAATTGCTTCAGGATTCATTTGGTTATCAGCTGCAATTACTGT
AGGTTTCTTATTTTGGATTATCTGGTACATCCTTTCGAATGGCATGCAACACGTAAGTTGGTCATTCATTACAGATAACT
ACACTCGAACAGGTGAAGAGCACGGTATCTTCCCTATGATAGTTTCAACGTTATACATGGTACTTGCTTCAATTGCTGTC
GCAGCACCACTTGGCATCATGACAGCTATTTACCTAACCGAATATGCAAAGGTAGGAAGCAAATTAGTTAAAGCCATTCG
CTTCTGTACTGAATCGTTAGCTGGCATTCCATCGATTATCTTTGGTCTGTTTGGTATGACTTTCTTTGTAGGTATTTTAG
GTCTTGGCTTCTCTATCCTATCTGGTGCTTTAACACTGAGTATTTTAATACTTCCTGTTATTATTCGTACAACAGAAGAA
GCGTTAATGGCTGTACCTCAAACATTTCGTGAAGGTTCATACGGACTTGGCGCATCAAAAATTTATACAATTTGGCGCTT
AATTTTACCAAGTGCAATGCCAGGTATTCTGACTTCCGTTATTTTAAGTATTGGCCGTGTTATTGGTGAGTCTGCACCCG
TGTTTTTGACCGCTGGTATGGTTGCACGTATTCCCGATTCATTACTGGATTCAGGACGTACACTAACCGTTCACCTTTAT
AAGTTAACCACTGAATTATTTACTATCGACGAGTGGAACCAAGCTTACGGAACTGCAACCGTACTTATCGTTCTTGTTCT
TGTCATTAATACGCTAACCAAGCTATTGGCTCGAAAAATTAATAAAGCATCTTACTAA

Upstream 100 bases:

>100_bases
GCCAACGCTCTGTACGCAACTGGTGTGGTATTGCTTGTATTTATCATGTCTCTTAATGGCATTCTTCTTTACTTAAACCG
TGAAACGGCGAAATAAGATC

Downstream 100 bases:

>100_bases
AATATAACGCTATCAAATTAAATCAGTGGCACGACAATAAGTTGCGGCTGATAAGGAAAATACAATGAACAAATTTAATA
TTGAAAATCTAGACCTTTTT

Product: phosphate ABC transporter, permease protein PstA

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNKLKKQRQIKDQIASGFIWLSAAITVGFLFWIIWYILSNGMQHVSWSFITDNYTRTGEEHGIFPMIVSTLYMVLASIAV
AAPLGIMTAIYLTEYAKVGSKLVKAIRFCTESLAGIPSIIFGLFGMTFFVGILGLGFSILSGALTLSILILPVIIRTTEE
ALMAVPQTFREGSYGLGASKIYTIWRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGMVARIPDSLLDSGRTLTVHLY
KLTTELFTIDEWNQAYGTATVLIVLVLVINTLTKLLARKINKASY

Sequences:

>Translated_285_residues
MNKLKKQRQIKDQIASGFIWLSAAITVGFLFWIIWYILSNGMQHVSWSFITDNYTRTGEEHGIFPMIVSTLYMVLASIAV
AAPLGIMTAIYLTEYAKVGSKLVKAIRFCTESLAGIPSIIFGLFGMTFFVGILGLGFSILSGALTLSILILPVIIRTTEE
ALMAVPQTFREGSYGLGASKIYTIWRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGMVARIPDSLLDSGRTLTVHLY
KLTTELFTIDEWNQAYGTATVLIVLVLVINTLTKLLARKINKASY
>Mature_285_residues
MNKLKKQRQIKDQIASGFIWLSAAITVGFLFWIIWYILSNGMQHVSWSFITDNYTRTGEEHGIFPMIVSTLYMVLASIAV
AAPLGIMTAIYLTEYAKVGSKLVKAIRFCTESLAGIPSIIFGLFGMTFFVGILGLGFSILSGALTLSILILPVIIRTTEE
ALMAVPQTFREGSYGLGASKIYTIWRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGMVARIPDSLLDSGRTLTVHLY
KLTTELFTIDEWNQAYGTATVLIVLVLVINTLTKLLARKINKASY

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=286, Percent_Identity=32.5174825174825, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790164, Length=231, Percent_Identity=25.974025974026, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31239; Mature: 31239

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKKQRQIKDQIASGFIWLSAAITVGFLFWIIWYILSNGMQHVSWSFITDNYTRTGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
HGIFPMIVSTLYMVLASIAVAAPLGIMTAIYLTEYAKVGSKLVKAIRFCTESLAGIPSII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLFGMTFFVGILGLGFSILSGALTLSILILPVIIRTTEEALMAVPQTFREGSYGLGASK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IYTIWRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGMVARIPDSLLDSGRTLTVHLY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEHHH
KLTTELFTIDEWNQAYGTATVLIVLVLVINTLTKLLARKINKASY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKLKKQRQIKDQIASGFIWLSAAITVGFLFWIIWYILSNGMQHVSWSFITDNYTRTGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
HGIFPMIVSTLYMVLASIAVAAPLGIMTAIYLTEYAKVGSKLVKAIRFCTESLAGIPSII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLFGMTFFVGILGLGFSILSGALTLSILILPVIIRTTEEALMAVPQTFREGSYGLGASK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IYTIWRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGMVARIPDSLLDSGRTLTVHLY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEHHH
KLTTELFTIDEWNQAYGTATVLIVLVLVINTLTKLLARKINKASY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]