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The map label for this gene is argT [H]

Identifier: 59712194

GI number: 59712194

Start: 1777668

End: 1778444

Strand: Reverse

Name: argT [H]

Synonym: VF_1587

Alternate gene names: 59712194

Gene position: 1778444-1777668 (Counterclockwise)

Preceding gene: 59712195

Following gene: 59712193

Centisome position: 61.38

GC content: 37.58

Gene sequence:

>777_bases
ATGAAAAAGTGGTTATTAGCAGCAGTTGTTGCATCAACAGCACTAACAGGCGCAGCTCAAGCAAAAGAATGGAAACAAAT
CCGTTTTGGTATTGAAGGCGCTTATCCTCCATTTAGTTGGACAGAACCTTCAGGTGAGCTTAAAGGTTTTGATGTGGATA
TTGCTAATGCGTTATGTAAGGAAATGGAAGCACGTTGTAAAATCGTAGCTCAAGATTGGGATGGTATTATTCCTTCATTA
CTTGCTCGTAAATATGATGCAATTATTGCCGCAATGTCTATTACAGAAGAGCGTAAAAAGAAGATTGATTTCACTGAAAA
ATATGCACTTATTCCAAATAAATTTGTCGCTAAAAAAGGCACAAAATTAGTAATGACTAAAGAAGGCCTTAAAGGGGTCA
CTGTTGGTGTTCAACGTGCAACAACACATGATAAATACCTAACTGACAACTTTGGTAAAGGTGTTGATCTTAAGCGCTAC
GGTTCATTTGATGAAGCCTATTTAGATCTAAAGGCGGGTCGTATTGATACGGTTTTAGGTGATGCATCAGCACTAGAAGA
AGGTCTTCTTAATAAACCTGGCGGTACAGAGTATGAATTTGTTGGTCCGTCACTAACTGATCCAAAATGGTTTGGTGAAG
GTTTTGGTATCGCAACACGTAAACAAGATAAGGATCTAACTGAGCAATTAAATGCTGCAATTGATTCTTTACGTGAAAAA
GGCATTTATCAAGAAATTGCTGCGAAATACTTTAACTATGATGTGTATGGTGAGTAA

Upstream 100 bases:

>100_bases
CAGCAATTTATTTCATCTATTTATTAAACATATTGTAAAGCAAACACTACCAATAAAAATTAAATCAATTAAACGGAATA
AAAGTTACAGGAGTAAGGAA

Downstream 100 bases:

>100_bases
TTGTTGAATTAGCCTTAAGGAAAGAGGGTGGAATATACCTCTTTCATTCTAATAGTTATAACAACGTCAATGAAGCGTTC
TTGATATTTATTGGCGTTGT

Product: lysine/arginine/ornithine transporter subunit

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKWLLAAVVASTALTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTKLVMTKEGLKGVTVGVQRATTHDKYLTDNFGKGVDLKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGTEYEFVGPSLTDPKWFGEGFGIATRKQDKDLTEQLNAAIDSLREK
GIYQEIAAKYFNYDVYGE

Sequences:

>Translated_258_residues
MKKWLLAAVVASTALTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTKLVMTKEGLKGVTVGVQRATTHDKYLTDNFGKGVDLKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGTEYEFVGPSLTDPKWFGEGFGIATRKQDKDLTEQLNAAIDSLREK
GIYQEIAAKYFNYDVYGE
>Mature_258_residues
MKKWLLAAVVASTALTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTKLVMTKEGLKGVTVGVQRATTHDKYLTDNFGKGVDLKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGTEYEFVGPSLTDPKWFGEGFGIATRKQDKDLTEQLNAAIDSLREK
GIYQEIAAKYFNYDVYGE

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=39.6153846153846, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=42.3076923076923, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=41.8326693227092, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=39.4422310756972, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=33.7552742616034, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=30.6772908366534, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28588; Mature: 28588

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLAAVVASTALTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMEARCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCC
TKLVMTKEGLKGVTVGVQRATTHDKYLTDNFGKGVDLKRYGSFDEAYLDLKAGRIDTVLG
CEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHC
DASALEEGLLNKPGGTEYEFVGPSLTDPKWFGEGFGIATRKQDKDLTEQLNAAIDSLREK
CHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GIYQEIAAKYFNYDVYGE
CHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKWLLAAVVASTALTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMEARCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCC
TKLVMTKEGLKGVTVGVQRATTHDKYLTDNFGKGVDLKRYGSFDEAYLDLKAGRIDTVLG
CEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHC
DASALEEGLLNKPGGTEYEFVGPSLTDPKWFGEGFGIATRKQDKDLTEQLNAAIDSLREK
CHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GIYQEIAAKYFNYDVYGE
CHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]