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The map label for this gene is xthA [H]

Identifier: 59712190

GI number: 59712190

Start: 1774258

End: 1775064

Strand: Direct

Name: xthA [H]

Synonym: VF_1583

Alternate gene names: 59712190

Gene position: 1774258-1775064 (Clockwise)

Preceding gene: 59712187

Following gene: 59712191

Centisome position: 61.23

GC content: 39.03

Gene sequence:

>807_bases
ATGAAAGTAATAAGCTTTAATATAAATGGCCTTCGTGCTCGCCTTCACCAATTACAAGCTATCATTGATAAGCACAACCC
TGATGTTATTGGTCTTCAAGAGATCAAAGTTCATAATGAAATGTTCCCAGTCGAAGCAGTAGAAGCGATGGGCTATAAAG
TGTATTTCCATGGCCAAAAAGCACATTACGGTGTGGCGATGCTATGTAAACAAGAGCCAATTTCTGTGCAATATGGTTTT
GATACGGATACAGAAGAACATCAAAAGCGCATGATCATGACTACTCACCAACGTGAAGATGGCTCAAAAGTAACGATAAT
GAATGGTTACTTCCCACAAGGTGATAACATCAAGCATGAAACTAAATTCCCTTATAAACGTCAATTCTACAAAGATTTGA
TGACTCACTTGCATACACACCATACTAATGATGAAGAGATCATCGTCATGGGCGATATTAATATCAGCCCCATCGATGCA
GATATCGGTATCGGTGAACCAAACGCAAAACGTTGGTTAAAAACAGGCAAATGCTCGTTCCAACCAGAAGAGCGTGAATG
GTTACAAACATTAAAAGATTGGGGGTTTGAAGACACATTCCGTAATCTTCACCCTGAAGTAACCGATCAATTCTCGTGGT
TTGATTATCGTTCAAAAGGGTTTGTAGATAACCGTGGTTTACGCATTGATGTCATTATGGCAACACCAACATTAGCAGTA
AAATGTACAGAATCTGGCATTGATTACGAATTACGTGGTATTGAAAAGCCTTCTGATCACGCACCAATTTGGTCAACATT
CAAATAA

Upstream 100 bases:

>100_bases
CTAAACTTACATGATTTAATGCCAAATAATGTTAGTATCATTATATTAGAGATCCTCGCTCAACTAGGATTGATTATATT
TATAAGGACTTGATAACCTA

Downstream 100 bases:

>100_bases
TATGTTTAGCCCTCACCTATTTGTGAGGGCTTTTTTATCCCAATCAATGGCACGATTGGTATTAAGGAAATACAATGAAA
TTATTCATCTTTGATCACTG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKVISFNINGLRARLHQLQAIIDKHNPDVIGLQEIKVHNEMFPVEAVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQYGF
DTDTEEHQKRMIMTTHQREDGSKVTIMNGYFPQGDNIKHETKFPYKRQFYKDLMTHLHTHHTNDEEIIVMGDINISPIDA
DIGIGEPNAKRWLKTGKCSFQPEEREWLQTLKDWGFEDTFRNLHPEVTDQFSWFDYRSKGFVDNRGLRIDVIMATPTLAV
KCTESGIDYELRGIEKPSDHAPIWSTFK

Sequences:

>Translated_268_residues
MKVISFNINGLRARLHQLQAIIDKHNPDVIGLQEIKVHNEMFPVEAVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQYGF
DTDTEEHQKRMIMTTHQREDGSKVTIMNGYFPQGDNIKHETKFPYKRQFYKDLMTHLHTHHTNDEEIIVMGDINISPIDA
DIGIGEPNAKRWLKTGKCSFQPEEREWLQTLKDWGFEDTFRNLHPEVTDQFSWFDYRSKGFVDNRGLRIDVIMATPTLAV
KCTESGIDYELRGIEKPSDHAPIWSTFK
>Mature_268_residues
MKVISFNINGLRARLHQLQAIIDKHNPDVIGLQEIKVHNEMFPVEAVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQYGF
DTDTEEHQKRMIMTTHQREDGSKVTIMNGYFPQGDNIKHETKFPYKRQFYKDLMTHLHTHHTNDEEIIVMGDINISPIDA
DIGIGEPNAKRWLKTGKCSFQPEEREWLQTLKDWGFEDTFRNLHPEVTDQFSWFDYRSKGFVDNRGLRIDVIMATPTLAV
KCTESGIDYELRGIEKPSDHAPIWSTFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=23.4200743494424, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=23.4200743494424, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=23.4200743494424, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=61.1940298507463, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330655, Length=272, Percent_Identity=30.1470588235294, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=272, Percent_Identity=30.1470588235294, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31173; Mature: 31173

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISFNINGLRARLHQLQAIIDKHNPDVIGLQEIKVHNEMFPVEAVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHEEEECCCCCCHHHHHHCCEEEEEECCC
AHYGVAMLCKQEPISVQYGFDTDTEEHQKRMIMTTHQREDGSKVTIMNGYFPQGDNIKHE
CCCCEEEEECCCCCEEEECCCCCHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYKDLMTHLHTHHTNDEEIIVMGDINISPIDADIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCC
QPEEREWLQTLKDWGFEDTFRNLHPEVTDQFSWFDYRSKGFVDNRGLRIDVIMATPTLAV
CCHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCEEEEEEECCEEEE
KCTESGIDYELRGIEKPSDHAPIWSTFK
EEECCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVISFNINGLRARLHQLQAIIDKHNPDVIGLQEIKVHNEMFPVEAVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHEEEECCCCCCHHHHHHCCEEEEEECCC
AHYGVAMLCKQEPISVQYGFDTDTEEHQKRMIMTTHQREDGSKVTIMNGYFPQGDNIKHE
CCCCEEEEECCCCCEEEECCCCCHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYKDLMTHLHTHHTNDEEIIVMGDINISPIDADIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCC
QPEEREWLQTLKDWGFEDTFRNLHPEVTDQFSWFDYRSKGFVDNRGLRIDVIMATPTLAV
CCHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCEEEEEEECCEEEE
KCTESGIDYELRGIEKPSDHAPIWSTFK
EEECCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]