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The map label for this gene is purR

Identifier: 59712179

GI number: 59712179

Start: 1760966

End: 1761979

Strand: Direct

Name: purR

Synonym: VF_1572

Alternate gene names: 59712179

Gene position: 1760966-1761979 (Clockwise)

Preceding gene: 59712178

Following gene: 59712180

Centisome position: 60.77

GC content: 38.66

Gene sequence:

>1014_bases
ATGGCTACTATTAAAGATGTGGCGAAGCTGGCAGCAGTTTCAACAACCACCGTTTCTCACGTGATAAATAAAACACGATT
TGTTGCTGAAGCAACACAAAAACGTGTTTGGGAAGCAGTTGAAGAATTAAACTATGCTCCAAGTGCGGTAGCACGTAGCT
TAAAGTGTAATACGACACGTACTATTGGTATGTTAGTAACACAATCATTTAACCCATTTTTTGCAGAAGTAATGCATGGT
GTTGAAAACTATTGTTACAAGCAAGGCTACACGCTATTCATGTGTAATACTGAAGGCGACCTAGAAAAACAAAAACACTA
TTTGAGAATGTTGGCGGAAAAACGAGTAGATGGCCTACTTGTAATGTGTTCAGATCTAAATGAACAATTACTGACCTTGC
TTGAAAAGAACACCGAGCTACCAATGGTTATTATGGACTGGGGTCCTGATAGCCCGCGTACAGACAAGATCATTGATAAT
TCAGAAGAAGGTGGTTATTTAGCGACGAAGCATCTTATTGAAAATGGTCACACTCATATTGCGTGCATTACAGGTCAAGC
TGATAAAGTGACTTGTAAAGAACGTGTTCGTGGCTTTGAAAGAGCTCATATCGATGCAAATTTAACCTTTAACCCAGAAT
GGATCTTAGAGGGCGATTTTGAATGTGCTTCTGCATCTAGAGCCGTTGATAAAATTCTCGCTATTGAAGAAAATAAACGC
CCTACAGCTTTATTCTGCTTTAACGATATTATGGCATTGGCTGCCATTAGCAAGATTCAACAATCTGGTTTACGTGTACC
TGAAGATATTTCAGTTATCGGTTATGACAACATTGAATTGTCTGCGTATTTCTCACCACCATTAACAACAATCCATCAAC
CTAAACGCCGTGTAGGTAAAACAGCAGTTGAAATTTTGTTAGAGCGAATTAAAGATAAAGATCATGAAAGACGCGTATTT
GAAATGCAACCAGAGGTTGTTACACGTAGTAGTGTTTCAAACCGCCTTAAATAA

Upstream 100 bases:

>100_bases
CCAGGGCCTATTTATCTTCCATCAGTTCTTCTAGTTTAGAGCGACACCTTTTTTTGTTGGAAAATAAATAGCCCCTTATT
ACCTATTATAAGGTTTATAT

Downstream 100 bases:

>100_bases
TCAATCTAACGCATAATTTGAGATTCAAAAAAAAGCCATACTATTAAAGTGTGGCTTTTTTTATTATTAAATATTGTTTA
CCTAATTCTCAATAATAAAA

Product: DNA-binding transcriptional repressor, hypoxanthine-binding

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEVMHG
VENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLLVMCSDLNEQLLTLLEKNTELPMVIMDWGPDSPRTDKIIDN
SEEGGYLATKHLIENGHTHIACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRAVDKILAIEENKR
PTALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKDHERRVF
EMQPEVVTRSSVSNRLK

Sequences:

>Translated_337_residues
MATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEVMHG
VENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLLVMCSDLNEQLLTLLEKNTELPMVIMDWGPDSPRTDKIIDN
SEEGGYLATKHLIENGHTHIACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRAVDKILAIEENKR
PTALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKDHERRVF
EMQPEVVTRSSVSNRLK
>Mature_336_residues
ATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEVMHGV
ENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLLVMCSDLNEQLLTLLEKNTELPMVIMDWGPDSPRTDKIIDNS
EEGGYLATKHLIENGHTHIACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRAVDKILAIEENKRP
TALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKDHERRVFE
MQPEVVTRSSVSNRLK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=52.5373134328358, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=47.1299093655589, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=34.0361445783133, Blast_Score=210, Evalue=8e-56,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=33.2203389830508, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=28.955223880597, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786540, Length=307, Percent_Identity=32.2475570032573, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=28.2828282828283, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=25.8461538461538, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=28.8461538461538, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790715, Length=334, Percent_Identity=24.5508982035928, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786268, Length=307, Percent_Identity=23.1270358306189, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=21.2574850299401, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_VIBF1 (Q5E4H9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204955.1
- HSSP:   P46828
- ProteinModelPortal:   Q5E4H9
- SMR:   Q5E4H9
- STRING:   Q5E4H9
- GeneID:   3278848
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1572
- NMPDR:   fig|312309.3.peg.1572
- eggNOG:   COG1609
- HOGENOM:   HBG753640
- OMA:   CFNDIMA
- PhylomeDB:   Q5E4H9
- ProtClustDB:   CLSK874518
- BioCyc:   VFIS312309:VF1572-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 38056; Mature: 37925

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 191-191 BINDING 220-220 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
TIGMLVTQSFNPFFAEVMHGVENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
VMCSDLNEQLLTLLEKNTELPMVIMDWGPDSPRTDKIIDNSEEGGYLATKHLIENGHTHI
EEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
ACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRAVDKILAIEENKR
EEEECCCCCCHHHHHHCCHHHHEECEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCC
PTALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGK
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCCHHHHCHHHHHHH
TAVEILLERIKDKDHERRVFEMQPEVVTRSSVSNRLK
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
TIGMLVTQSFNPFFAEVMHGVENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
VMCSDLNEQLLTLLEKNTELPMVIMDWGPDSPRTDKIIDNSEEGGYLATKHLIENGHTHI
EEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
ACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRAVDKILAIEENKR
EEEECCCCCCHHHHHHCCHHHHEECEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCC
PTALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGK
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCCHHHHCHHHHHHH
TAVEILLERIKDKDHERRVFEMQPEVVTRSSVSNRLK
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA