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The map label for this gene is nrfA [H]

Identifier: 59712161

GI number: 59712161

Start: 1737759

End: 1739195

Strand: Direct

Name: nrfA [H]

Synonym: VF_1554

Alternate gene names: 59712161

Gene position: 1737759-1739195 (Clockwise)

Preceding gene: 59712154

Following gene: 59712165

Centisome position: 59.97

GC content: 44.19

Gene sequence:

>1437_bases
GTGAAAAAGCAATGGACCCGTCGTTCAGCCGCTGCAATAGCAATGGTGACTACTTTATTATTAAGTAGCCACAGCTTCGC
TGCCTCTGAAAACAATGAACGAGTTGACCCTCGTAATGAAGCCTACGCAAAAGATCATGCCGATCAATATGATTCATGGC
GATCTACCTCTGATAGTGTGCAAATCGAAGATGCATTAGCTGAAGATCCTAATATGGTGATCTTATGGGCTGGCTACGGT
TTTGCAAAAGACTACAACAAAGCTCGTGGACACTTTTATGCTGTCGATGATGTAAGACAAACCCTTCGTACTGGTGGCCC
AACCGATGAAAATTCAGGCCCGATGCCAATGGCATGTTGGAGCTGTAAAAGCCCAGATGTTGGCCGAGTTATCGAAGAAC
AAGGCGAAGATGGTTATTTCTCAGGAAAATGGGCTCGCTTAGGCAGTGAAATCCAAAACCCTATCGGCTGTGCAGATTGC
CATGATACTCGTAGTGAAGAATTTAAAAATGGTGGCCCTGCTCTTGCCTTAACTAAACCTCACGTTGAGCGTGCAATGGA
TGCCATTGGTAAACCTTTTGGTGATCAATCTCGTCTTGATCAACAAGCTGCGGTGTGTGGTCAGTGTCACGTAGAATACT
ACTTCACAGGTAAAACTAAAGCCGTAAAATTCCCTTGGGACAAAGGCACTACCGTTGACGAAATGGAAGAATACTATGAT
GAGATCGGCTTTAAAGACTGGACTCACAAAGTATCTAAAGCGCCTATGTTAAAAGCGCAGCATCCTGGTTATGAAACATG
GCGTGATGGTATTCATGGCAAAAACAAAGTAACTTGTGTTGACTGTCATATGCCTAAAGTAACCAAAGAAGATGGCACGG
TGTACACAGACCATAAAGTAGGTAACCCATTTGATCGTTTTGAAGATACCTGTGCAAATTGTCACACTCAATCTAAAGAA
ATGCTTCAAAGTGTTGTTGCAACCCGTAAAGCACAAGTGCTTAAAATGAAACTGACAGCAGAGCGTCAAATCGTTGCTGC
TCACTTTGAAGCTGGTGCAGCATGGGATGCTGGTGCTACTGAAAAAGAAATGGCACCAATCCTACAAGACATTCGCCACG
CACAATGGCGTTGGGATTATGCGATTGCTTCGCATGGTGTTCATATGCACGCACCAGAAGTTGCTCTAGAAGTGTTAGGT
ACCGCTGTCGATAAAGCAGCAGACGCTCGAACTAAACTTGTGCGCTTACTTGCGAAAAAAGGCATTACAGAGCCAGTAGC
TATTCCTGATATTTCAACGAAAGCAGCAGCACAAAAAGCATTAGGAATGGACATGGAAGCGATGAAAGCTGAGAAGAAAC
ACTTCTTAGAAACCGTTGTTCCAGAGTGGGAAGAACAAGCAAAAGAACGTGAAGCCCGAGATTACGAACCAATGTAA

Upstream 100 bases:

>100_bases
ACCTCTTAATTAGTATATAATTTACCTGTTTCCAATTCTGATTGATGGTCGCTTTCACAACGCTTGGCTAATTAATTAAA
AGAATATTGGAGATACAATA

Downstream 100 bases:

>100_bases
TTACGACTAGCTTAAACAGGTAGTAATAAATAAAAAGCCGACAGTTAAGTCGGCTTTTTTATGTTTCTTATTTATTAGTA
CAACTTATAAAGTTTAATTA

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASENNERVDPRNEAYAKDHADQYDSWRSTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAVDDVRQTLRTGGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALALTKPHVERAMDAIGKPFGDQSRLDQQAAVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFKDWTHKVSKAPMLKAQHPGYETWRDGIHGKNKVTCVDCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKE
MLQSVVATRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATEKEMAPILQDIRHAQWRWDYAIASHGVHMHAPEVALEVLG
TAVDKAADARTKLVRLLAKKGITEPVAIPDISTKAAAQKALGMDMEAMKAEKKHFLETVVPEWEEQAKEREARDYEPM

Sequences:

>Translated_478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASENNERVDPRNEAYAKDHADQYDSWRSTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAVDDVRQTLRTGGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALALTKPHVERAMDAIGKPFGDQSRLDQQAAVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFKDWTHKVSKAPMLKAQHPGYETWRDGIHGKNKVTCVDCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKE
MLQSVVATRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATEKEMAPILQDIRHAQWRWDYAIASHGVHMHAPEVALEVLG
TAVDKAADARTKLVRLLAKKGITEPVAIPDISTKAAAQKALGMDMEAMKAEKKHFLETVVPEWEEQAKEREARDYEPM
>Mature_478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASENNERVDPRNEAYAKDHADQYDSWRSTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAVDDVRQTLRTGGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALALTKPHVERAMDAIGKPFGDQSRLDQQAAVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFKDWTHKVSKAPMLKAQHPGYETWRDGIHGKNKVTCVDCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKE
MLQSVVATRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATEKEMAPILQDIRHAQWRWDYAIASHGVHMHAPEVALEVLG
TAVDKAADARTKLVRLLAKKGITEPVAIPDISTKAAAQKALGMDMEAMKAEKKHFLETVVPEWEEQAKEREARDYEPM

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=435, Percent_Identity=65.9770114942529, Blast_Score=631, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 53447; Mature: 53447

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQWTRRSAAAIAMVTTLLLSSHSFAASENNERVDPRNEAYAKDHADQYDSWRSTSDSV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
QIEDALAEDPNMVILWAGYGFAKDYNKARGHFYAVDDVRQTLRTGGPTDENSGPMPMACW
EEHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEE
SCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADCHDTRSEEFKNGGPALALTKP
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEECH
HVERAMDAIGKPFGDQSRLDQQAAVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
HHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHH
EIGFKDWTHKVSKAPMLKAQHPGYETWRDGIHGKNKVTCVDCHMPKVTKEDGTVYTDHKV
HCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCC
GNPFDRFEDTCANCHTQSKEMLQSVVATRKAQVLKMKLTAERQIVAAHFEAGAAWDAGAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EKEMAPILQDIRHAQWRWDYAIASHGVHMHAPEVALEVLGTAVDKAADARTKLVRLLAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITEPVAIPDISTKAAAQKALGMDMEAMKAEKKHFLETVVPEWEEQAKEREARDYEPM
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKQWTRRSAAAIAMVTTLLLSSHSFAASENNERVDPRNEAYAKDHADQYDSWRSTSDSV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
QIEDALAEDPNMVILWAGYGFAKDYNKARGHFYAVDDVRQTLRTGGPTDENSGPMPMACW
EEHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEE
SCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADCHDTRSEEFKNGGPALALTKP
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEECH
HVERAMDAIGKPFGDQSRLDQQAAVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
HHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHH
EIGFKDWTHKVSKAPMLKAQHPGYETWRDGIHGKNKVTCVDCHMPKVTKEDGTVYTDHKV
HCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCC
GNPFDRFEDTCANCHTQSKEMLQSVVATRKAQVLKMKLTAERQIVAAHFEAGAAWDAGAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EKEMAPILQDIRHAQWRWDYAIASHGVHMHAPEVALEVLGTAVDKAADARTKLVRLLAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITEPVAIPDISTKAAAQKALGMDMEAMKAEKKHFLETVVPEWEEQAKEREARDYEPM
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA