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The map label for this gene is tusE [H]

Identifier: 59712108

GI number: 59712108

Start: 1662488

End: 1662814

Strand: Direct

Name: tusE [H]

Synonym: VF_1501

Alternate gene names: 59712108

Gene position: 1662488-1662814 (Clockwise)

Preceding gene: 59712105

Following gene: 59712110

Centisome position: 57.38

GC content: 39.14

Gene sequence:

>327_bases
ATGGAATTTAACGGTAAGCAAATCGAGCGTGACGCTCAAGGGTATTTAAAAAACCACACAGATTGGTGTGAAGAGTTAGT
TCCACTTCTAGCAAAAGAAGAGAATATTGAACTGACCGAAGCTCACTGGGAAGTAGTACGTTTCGTTCGTAACTTTTACG
ATGAATTTAAAACCTCTCCAGCAGTTCGCATGCTTGTAAAAGCAATGGAAAAAGAGCATGGCCCTGAAAAAGGTAACAGC
AAATACCTATTTCAATTATTTAAGTTAGGACCTGCAAAACAAGCGACAAAATTAGCGGGTTTACCGAAACCGGCAAAATG
TCTATAA

Upstream 100 bases:

>100_bases
ACAAAAATCTGTTTTACTTAAATGTAATAATTCTCGATTGCTCGAACTAATACCTATTTGCTAAACTGAATAGATTAATC
CCAACCATTAAGACAGTCGT

Downstream 100 bases:

>100_bases
CATCGCTCTTATTGCTTAACTTTGGCAAGAATTAATACATTTTAAAATCCGTTAATTCTTGCCATTGTGTCTCTTCTTGC
TTTATTGAATCCACTCTCGC

Product: sulfur transfer protein TusE

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E [H]

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MEFNGKQIERDAQGYLKNHTDWCEELVPLLAKEENIELTEAHWEVVRFVRNFYDEFKTSPAVRMLVKAMEKEHGPEKGNS
KYLFQLFKLGPAKQATKLAGLPKPAKCL

Sequences:

>Translated_108_residues
MEFNGKQIERDAQGYLKNHTDWCEELVPLLAKEENIELTEAHWEVVRFVRNFYDEFKTSPAVRMLVKAMEKEHGPEKGNS
KYLFQLFKLGPAKQATKLAGLPKPAKCL
>Mature_108_residues
MEFNGKQIERDAQGYLKNHTDWCEELVPLLAKEENIELTEAHWEVVRFVRNFYDEFKTSPAVRMLVKAMEKEHGPEKGNS
KYLFQLFKLGPAKQATKLAGLPKPAKCL

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD [H]

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family [H]

Homologues:

Organism=Escherichia coli, GI87081811, Length=106, Percent_Identity=63.2075471698113, Blast_Score=146, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007453 [H]

Pfam domain/function: PF04358 DsrC [H]

EC number: NA

Molecular weight: Translated: 12495; Mature: 12495

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNGKQIERDAQGYLKNHTDWCEELVPLLAKEENIELTEAHWEVVRFVRNFYDEFKTSP
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
AVRMLVKAMEKEHGPEKGNSKYLFQLFKLGPAKQATKLAGLPKPAKCL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEFNGKQIERDAQGYLKNHTDWCEELVPLLAKEENIELTEAHWEVVRFVRNFYDEFKTSP
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
AVRMLVKAMEKEHGPEKGNSKYLFQLFKLGPAKQATKLAGLPKPAKCL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA