| Definition | Vibrio fischeri ES114 chromosome I, complete genome. |
|---|---|
| Accession | NC_006840 |
| Length | 2,897,536 |
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The map label for this gene is helD [H]
Identifier: 59712071
GI number: 59712071
Start: 1621074
End: 1622471
Strand: Direct
Name: helD [H]
Synonym: VF_1464
Alternate gene names: 59712071
Gene position: 1621074-1622471 (Clockwise)
Preceding gene: 59712066
Following gene: 59712072
Centisome position: 55.95
GC content: 37.98
Gene sequence:
>1398_bases ATGACTTCATGGATTCTTTCAACAAACCCGATTGCTCGTCTCTTTAAAAAAGAAGCGGTTGTAACAATAGATAATGATGG AATACGTATTGTTCAGGCCGAAAATGAAACCATCATTAAATGGGATCAACTAGATTCCCCACCCAATCTTTCACTTTCTA TTGATGGCGGTTGTTTAACGTTTATCTCGCAAGGAAAAAACCATCGCTACCGAATGTTGGGATATTTTACGCCTTTTAAA TACGCCAAGCGTTTCTTTCCCTTCTGGGCGAATCAAAATGCAGAACGTTTACAAGATTTTCTCAGTGATGCTTACATTCA GTGCACATCAACGTTTCTTAGAGACTCTTCGATAGAAAATATCCGTGCAGTAGTACGTAATGAAATTAAACGATGGAAAG GTTGGGATAAGGTTGAAGGATTATCTGAGCTTGCGCATAAAACGGTAACTCAACTTACCAATATTCATCATTGGTCGTCA GCTGATATTGAGAAGATTCGCCAACGCTATGTGAACAAGCAATTAGCTCAATACCAAACCTTTTTTGATAGCGTTGAATC TAACCCTTTAACCAATAGACAAAGAATCGCTTGTGTTACTGATAATGACAACAACCTCTTACTTGCTGGCGCAGGAACAG GTAAAACAAGCGTTATGATCGGTAAAGCTGGGTATTTGGTTAATAGCGGTAAAGCAAGCCCAAAGCAGATCTTAATGCTC GCTTATGGTCGAATTGCTGCTCAAGAAATGAATGAGCGAATCAAAGAAAAACTGGGCTTTGATGATGTAAAAGCGAGTAC CTTTCACAGCCTTGGTGTAAAAATAATCTCTGAAGTAGAAGGCAAAGCCCCTAGCCTATCTAAGCTTGAAGATAATCCAA AAGTTAAAGCCAAATGGATGCACGATGAGATTGAAATTCTTATGCGTGATAATAACTATAGAAAGGCGTTACTTGATTAT TTTAGTTCTTATTATTTCGTTGATAAAAACCCGTGGGAATTTGAATCTCAAGGTGCTTACCTTAAATATCTAAATGATAA TGAAGTTCAAACCATGAATGGCGAGAAGGTAAAAAGCTACGGTGAGCTTGTTGTGGCCAATTGGTTATTTAGAAAAGGAA TTAAGTATCAATATGAAGCCAAATACCGCTTTGATGTAGCTACAGAGAAGTACCGTCAATACGAGCCTGATTTTTATCTC CCTGATTATGATATCTACATTGAATATTACGGTACTGATGAAAATGGGGATACCGCACCATACATAAATAGAGAACGCTA TCAGCAAGGTATTGAATGGAAACGCAAGACACATAAGCAATATGGCACAGGTTATGTTGAGGTTTTTTACCATCAGCATA AAAAAAGAAAACTGCCACAAGCACTTGAGAAAGAGTGA
Upstream 100 bases:
>100_bases AATTCACAAAAGTTAACTTGGCTCAAATACAGTCCGTTATTTATTACGCTAGACTACAGCTAATTTTCGAGTCACTTCAC ACTTTATCTGGCCGTACATA
Downstream 100 bases:
>100_bases CTCGCTCGAGGCGTAAAATTAGAGCCTGTCTCTGATAACGCCTTACTTGATAATTTAGAACAACTGGGACAAGTAACAGA GTTAGCAAAATTATTAGGTG
Product: superfamily I DNA/RNA helicase
Products: NA
Alternate protein names: 75 kDa helicase [H]
Number of amino acids: Translated: 465; Mature: 464
Protein sequence:
>465_residues MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFK YAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSS ADIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDY FSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYL PDYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE
Sequences:
>Translated_465_residues MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFK YAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSS ADIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDY FSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYL PDYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE >Mature_464_residues TSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFKY AKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSA DIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILMLA YGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDYF SSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLP DYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE
Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]
Homologues:
Organism=Escherichia coli, GI1787196, Length=139, Percent_Identity=47.4820143884892, Blast_Score=131, Evalue=1e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000212 - InterPro: IPR014016 - InterPro: IPR022161 - InterPro: IPR003661 [H]
Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 54298; Mature: 54167
Theoretical pI: Translated: 9.02; Mature: 9.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLT CCCEEECCCHHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEECCCEEE FISQGKNHRYRMLGYFTPFKYAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIEN EHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH IRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSADIEKIRQRYVNKQLAQYQT HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH FFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML HHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHH AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWM HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEE HDEIEILMRDNNYRKALLDYFSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSY CCEEEEEEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEECCCCEEECCCHHHHHH GELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLPDYDIYIEYYGTDENGDTAP HHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCC YINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCHHHHCCC >Mature Secondary Structure TSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLT CCEEECCCHHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEECCCEEE FISQGKNHRYRMLGYFTPFKYAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIEN EHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH IRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSADIEKIRQRYVNKQLAQYQT HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH FFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML HHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHH AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWM HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEE HDEIEILMRDNNYRKALLDYFSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSY CCEEEEEEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEECCCCEEECCCHHHHHH GELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLPDYDIYIEYYGTDENGDTAP HHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCC YINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2542273; 8905232; 9278503 [H]