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The map label for this gene is 59712064

Identifier: 59712064

GI number: 59712064

Start: 1612714

End: 1614198

Strand: Direct

Name: 59712064

Synonym: VF_1457

Alternate gene names: NA

Gene position: 1612714-1614198 (Clockwise)

Preceding gene: 172087713

Following gene: 59712065

Centisome position: 55.66

GC content: 42.9

Gene sequence:

>1485_bases
ATGAGTCATTATCAGAAACTTGTTGCTCACAGTTTAAAAATATCTCGTTTTGAACACTTATCTGCAATTTGCGGTTGGGA
TCAAGCCGCTGTAATGCCATCAGGCGGTAACGACGCTCGTTCAGAAGCCATGGCCGAGCTTTCTGTACATCTTCATAAAC
TGGGTACAGAACCACAACTAGCGGAATGGTTTGATAAAGCAGAATCAGAATCTTTATCTGCTAATGAACAAGCAAGCCTT
CGTGAAATGAAATCAAAATGGCAACAAACCACAGCCCTACCTGAAACGCTAGTAGAAGCACAATCGTTAGCAGGCTCTAA
ATGTGAGCACGCTTGGCGCTCACAACGCAGTGAAAACGACTGGGATGGCTTTAGTAAAAACTTTGCAGAAGTCGTTGCCC
TATCTCAAGAAGAGGCACAAATCCGAGCGGAGATCACAGGCCTAACCCCTTATGATGCCATGCTTGATTTATATGAGCCG
GGAACCACCACAGCAAAACTGGATGTGGTATTTAATGACGTAAAATCATGGCTACCAGCGCTCATCCAAAACATCCAAGA
GAAACAAGCAACAGAGTCTATCTATTTGCCTCAAGGTCATTACCCAACCGAGCAACAACGCCAGTTGAGTTTGGATGTAA
TGAAGTTTTTAAACTTTGATTTTAACCATGGTCGTCTTGATGTCAGCATGCACCCTTTCTGTGGCGGCGTACCAACTGAT
GTTCGTATAACCACGCGTTACGATGAAAACGATTTTGTGCAATCTTTGATGGGAACCGTTCACGAGACAGGACACGCTCG
TTACGAACAAGGTTTACCAAAAGAATTTGCAGGCCTTGCCGTTGGTGAAGCACGCTCAATGGGGATTCACGAATCTCAAA
GCCTGTTTTTTGAAATGCAGTTAGGCCGCAGTGCTGAGTTTATTTCTCAACTATCACCATTAGTGCAAAAGAACTTTGTG
AATGCGGATCAGAAGATCTTTGAAACAGATAACCTGCAAAAAATCTACACTCGCGTAAAACCTGATTTTATCCGTGTCGA
TGCCGATGAAGCAACATACCCTGCTCACGTTATTTTACGTTATGAAATCGAACGTGATTTAATGAATGGTAACATCAGCT
ATAAAGATATCCCTGAACTGTGGGATGCAAAAATGCAGCAATACCTAGGTTTAACCACCAAAGGCAATTACAAAAACGGT
TGTATGCAAGACATTCACTGGACAGACGGCAGCTTTGGTTACTTCCCAAGTTACACATTAGGTGCCATGTACGCAGCACA
GTTTATGTCAGCAATGAAACAAACCGTAGATGTAGAGAAAGCCACACTAAGCGGTGATTTAAGCCCTATTTTCACTTGGT
TATCAGATAATATCTGGAGCAAGGCATCACTATTCTCAACCGATGATTTAGTGAAGCAGGCAACGGGTGATGTGCTAAAT
GCGAGTTTCTTTAAAGCACACTTAGAGAAGCGTTATTTGGGATAA

Upstream 100 bases:

>100_bases
TTTTGATATTTTAAAAATCACTGAATAACTCACTACCCTATCTTAAGACAATCGCGATATCATGAGCCTTAACTAAAAAA
CCTTACAAGGAACTAGCCTC

Downstream 100 bases:

>100_bases
AAGCTAGACGTTAATTCGCAGCTTTACTCTAAGTAATTAAGAAAATGGATGTAGTTTTGGCTGCATCCATTTTTTGTTCA
ATATCTAATAAACCATTCCT

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSHYQKLVAHSLKISRFEHLSAICGWDQAAVMPSGGNDARSEAMAELSVHLHKLGTEPQLAEWFDKAESESLSANEQASL
REMKSKWQQTTALPETLVEAQSLAGSKCEHAWRSQRSENDWDGFSKNFAEVVALSQEEAQIRAEITGLTPYDAMLDLYEP
GTTTAKLDVVFNDVKSWLPALIQNIQEKQATESIYLPQGHYPTEQQRQLSLDVMKFLNFDFNHGRLDVSMHPFCGGVPTD
VRITTRYDENDFVQSLMGTVHETGHARYEQGLPKEFAGLAVGEARSMGIHESQSLFFEMQLGRSAEFISQLSPLVQKNFV
NADQKIFETDNLQKIYTRVKPDFIRVDADEATYPAHVILRYEIERDLMNGNISYKDIPELWDAKMQQYLGLTTKGNYKNG
CMQDIHWTDGSFGYFPSYTLGAMYAAQFMSAMKQTVDVEKATLSGDLSPIFTWLSDNIWSKASLFSTDDLVKQATGDVLN
ASFFKAHLEKRYLG

Sequences:

>Translated_494_residues
MSHYQKLVAHSLKISRFEHLSAICGWDQAAVMPSGGNDARSEAMAELSVHLHKLGTEPQLAEWFDKAESESLSANEQASL
REMKSKWQQTTALPETLVEAQSLAGSKCEHAWRSQRSENDWDGFSKNFAEVVALSQEEAQIRAEITGLTPYDAMLDLYEP
GTTTAKLDVVFNDVKSWLPALIQNIQEKQATESIYLPQGHYPTEQQRQLSLDVMKFLNFDFNHGRLDVSMHPFCGGVPTD
VRITTRYDENDFVQSLMGTVHETGHARYEQGLPKEFAGLAVGEARSMGIHESQSLFFEMQLGRSAEFISQLSPLVQKNFV
NADQKIFETDNLQKIYTRVKPDFIRVDADEATYPAHVILRYEIERDLMNGNISYKDIPELWDAKMQQYLGLTTKGNYKNG
CMQDIHWTDGSFGYFPSYTLGAMYAAQFMSAMKQTVDVEKATLSGDLSPIFTWLSDNIWSKASLFSTDDLVKQATGDVLN
ASFFKAHLEKRYLG
>Mature_493_residues
SHYQKLVAHSLKISRFEHLSAICGWDQAAVMPSGGNDARSEAMAELSVHLHKLGTEPQLAEWFDKAESESLSANEQASLR
EMKSKWQQTTALPETLVEAQSLAGSKCEHAWRSQRSENDWDGFSKNFAEVVALSQEEAQIRAEITGLTPYDAMLDLYEPG
TTTAKLDVVFNDVKSWLPALIQNIQEKQATESIYLPQGHYPTEQQRQLSLDVMKFLNFDFNHGRLDVSMHPFCGGVPTDV
RITTRYDENDFVQSLMGTVHETGHARYEQGLPKEFAGLAVGEARSMGIHESQSLFFEMQLGRSAEFISQLSPLVQKNFVN
ADQKIFETDNLQKIYTRVKPDFIRVDADEATYPAHVILRYEIERDLMNGNISYKDIPELWDAKMQQYLGLTTKGNYKNGC
MQDIHWTDGSFGYFPSYTLGAMYAAQFMSAMKQTVDVEKATLSGDLSPIFTWLSDNIWSKASLFSTDDLVKQATGDVLNA
SFFKAHLEKRYLG

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 55821; Mature: 55690

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYQKLVAHSLKISRFEHLSAICGWDQAAVMPSGGNDARSEAMAELSVHLHKLGTEPQL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
AEWFDKAESESLSANEQASLREMKSKWQQTTALPETLVEAQSLAGSKCEHAWRSQRSEND
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
WDGFSKNFAEVVALSQEEAQIRAEITGLTPYDAMLDLYEPGTTTAKLDVVFNDVKSWLPA
CCHHHHHHHHHHHHCHHHHEEHHEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
LIQNIQEKQATESIYLPQGHYPTEQQRQLSLDVMKFLNFDFNHGRLDVSMHPFCGGVPTD
HHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
VRITTRYDENDFVQSLMGTVHETGHARYEQGLPKEFAGLAVGEARSMGIHESQSLFFEMQ
EEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHCCCCCCHHEEEEEC
LGRSAEFISQLSPLVQKNFVNADQKIFETDNLQKIYTRVKPDFIRVDADEATYPAHVILR
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEE
YEIERDLMNGNISYKDIPELWDAKMQQYLGLTTKGNYKNGCMQDIHWTDGSFGYFPSYTL
EEHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
GAMYAAQFMSAMKQTVDVEKATLSGDLSPIFTWLSDNIWSKASLFSTDDLVKQATGDVLN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHCHHHCCCHHHHHHHHCCCHHH
ASFFKAHLEKRYLG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SHYQKLVAHSLKISRFEHLSAICGWDQAAVMPSGGNDARSEAMAELSVHLHKLGTEPQL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
AEWFDKAESESLSANEQASLREMKSKWQQTTALPETLVEAQSLAGSKCEHAWRSQRSEND
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
WDGFSKNFAEVVALSQEEAQIRAEITGLTPYDAMLDLYEPGTTTAKLDVVFNDVKSWLPA
CCHHHHHHHHHHHHCHHHHEEHHEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
LIQNIQEKQATESIYLPQGHYPTEQQRQLSLDVMKFLNFDFNHGRLDVSMHPFCGGVPTD
HHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
VRITTRYDENDFVQSLMGTVHETGHARYEQGLPKEFAGLAVGEARSMGIHESQSLFFEMQ
EEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHCCCCCCHHEEEEEC
LGRSAEFISQLSPLVQKNFVNADQKIFETDNLQKIYTRVKPDFIRVDADEATYPAHVILR
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEE
YEIERDLMNGNISYKDIPELWDAKMQQYLGLTTKGNYKNGCMQDIHWTDGSFGYFPSYTL
EEHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
GAMYAAQFMSAMKQTVDVEKATLSGDLSPIFTWLSDNIWSKASLFSTDDLVKQATGDVLN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHCHHHCCCHHHHHHHHCCCHHH
ASFFKAHLEKRYLG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]