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The map label for this gene is mscS [H]

Identifier: 59712062

GI number: 59712062

Start: 1609798

End: 1611483

Strand: Direct

Name: mscS [H]

Synonym: VF_1455

Alternate gene names: 59712062

Gene position: 1609798-1611483 (Clockwise)

Preceding gene: 59712061

Following gene: 172087713

Centisome position: 55.56

GC content: 38.67

Gene sequence:

>1686_bases
ATGCGACATTTTATTTGGATCTTACTCTTCTTTAGTTCACTTTCCTTTGCTAGCGAAGCACAAGACTTAAGTCATTTAGA
GAAAATTAATGCGGAAATTGCACGACTAAAAACCGAGTCAAACTCATTTAGCGGCAGTGAATTGGCTATTATTCGAGTTC
AACAAGTCGAAAAAAATAATGAACTGCGATCTGTTTTATCACAGCTAATTCAAGAACATGACAAAACAACAAAAGAGCTT
TTAATCTCAGAGGTGAATAATCAAATTGGTTATGCAGAGCGATCTTTTGCCTATTTAGATAAAGAACTAAAATCAAAACA
AGCACAACTTGATAAAGCTGACATTGAACATAAGCTAGCGCTTCAAAACACATTAAAAGAAACCCGAGACTATTATTACC
TATCCCTTAGCGACCAGCTTGAAAACTACCAATGGCTCGCAGATCTTGGTGAATCTAACGAAGCTCAAATCGATGCGTTC
AAATTACTCATTGATCAAAAAATCAAATTTCTTTCTGCTTCATTAAGCTTTGATGCTAGCCGTGAACAACGTTTATCCGA
CCAACTTCAACACTCTTCTGAATCAGAAAAATCATCATTAACCCTAGATCACCTGTTCTTTGAGCGTAAAGTATTTAACT
CTACATCAGGGTTAGAAAAACTGATCAGTATTGCCGATCAGTTGGACATCAATACCACCGACTATAAACGTCAGCTATTT
TCGACAACGGGTAACCTAACAGAAGATATTCTTAAGTTCCCTGTTATTGCTGCCATCTTAAGTAATTGGATATCTTCAGG
TAGTAACTGGCTACTTAAACACGCTCCGGATCAGCTATTAAAATTCTTAGTCTTTATTCTGATCTTATTTGCTACCCGCT
TAATTGCCCGTTTAACAAGCCGCGTGGTATTAAAAGCAGTTGTTCAGCCTCATTTACGCATGAGTAAATTGATGCAAGAC
TTCTTTGTATCCATGTCGAGCAAGCTCATTTACTTTATTGGTATCCTAATCGCCTTTTCAAAAATCGGATTAGATCTGGC
ACCTGTATTAACAGGTTTCGGTGTTGCCGGTATTATTATTGGTTTCGCATTACAAGATACGTTATCTAACTTCGCATCGG
GCATGATGCTACTTATCTATCGCCCATTCGATGTCGGTGATTTAGTAGAAGCGGGTAGCGTATCAGGCAAAGTAAGCCAC
ATGAGCCTAGTAAACACCACCATCAAAACCTTTGATAACCAAATCATTATTCTACCTAACAGTATGATTTGGGGGGATGT
AATCAAAAACGTCACCCACGAGAGAGTGAGACGTGTGGACATGGTATTTGGGATAGGATACGAAGACAGCATAGAGCACG
CAGAGAAAATCTTAGCTGAGATTGTAGACGCACACCCTGCAGCATTGAAAAAACCAGAACCAAACATCAGAGTCCACACA
TTAGGTGCTTCTTCTGTTGATTTCATTGTTCGTCCTTGGGTAAAAACCGATGACTATTGGGATGTGTATTGGGACATTAC
TCGTGAAGTAAAACTGCGTTTTGATAAAGAAGGGATTTCGATTCCATTCGCACAGCAAGATGTGCACGTTCACTTTGCGA
AGAAAGATAAAAAACCAAAGAAGCCAAATCCAATTACCGAGAAAATTGGTAACGTGATTGACTCAGTAAGATCAAAAGAC
AGCTAA

Upstream 100 bases:

>100_bases
CTTTTTCATTTTTGTGACTACCAGTTAAAAAGTTATCCTGCCACTCTTGATAAAATTTCACTACAATAGGTCTAGCTTAA
TTCACAGTTATCGAGGCTCT

Downstream 100 bases:

>100_bases
ACAAAAGTGCCATGCGTATTCAATAATAGGCATGGCACTGTTTCATTCATCTTTTATATCAAGCTTGACACTTTAAAAAC
AAACTATTTAGAAACAAGTA

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRHFIWILLFFSSLSFASEAQDLSHLEKINAEIARLKTESNSFSGSELAIIRVQQVEKNNELRSVLSQLIQEHDKTTKEL
LISEVNNQIGYAERSFAYLDKELKSKQAQLDKADIEHKLALQNTLKETRDYYYLSLSDQLENYQWLADLGESNEAQIDAF
KLLIDQKIKFLSASLSFDASREQRLSDQLQHSSESEKSSLTLDHLFFERKVFNSTSGLEKLISIADQLDINTTDYKRQLF
STTGNLTEDILKFPVIAAILSNWISSGSNWLLKHAPDQLLKFLVFILILFATRLIARLTSRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIYFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVEAGSVSGKVSH
MSLVNTTIKTFDNQIIILPNSMIWGDVIKNVTHERVRRVDMVFGIGYEDSIEHAEKILAEIVDAHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTDDYWDVYWDITREVKLRFDKEGISIPFAQQDVHVHFAKKDKKPKKPNPITEKIGNVIDSVRSKD
S

Sequences:

>Translated_561_residues
MRHFIWILLFFSSLSFASEAQDLSHLEKINAEIARLKTESNSFSGSELAIIRVQQVEKNNELRSVLSQLIQEHDKTTKEL
LISEVNNQIGYAERSFAYLDKELKSKQAQLDKADIEHKLALQNTLKETRDYYYLSLSDQLENYQWLADLGESNEAQIDAF
KLLIDQKIKFLSASLSFDASREQRLSDQLQHSSESEKSSLTLDHLFFERKVFNSTSGLEKLISIADQLDINTTDYKRQLF
STTGNLTEDILKFPVIAAILSNWISSGSNWLLKHAPDQLLKFLVFILILFATRLIARLTSRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIYFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVEAGSVSGKVSH
MSLVNTTIKTFDNQIIILPNSMIWGDVIKNVTHERVRRVDMVFGIGYEDSIEHAEKILAEIVDAHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTDDYWDVYWDITREVKLRFDKEGISIPFAQQDVHVHFAKKDKKPKKPNPITEKIGNVIDSVRSKD
S
>Mature_561_residues
MRHFIWILLFFSSLSFASEAQDLSHLEKINAEIARLKTESNSFSGSELAIIRVQQVEKNNELRSVLSQLIQEHDKTTKEL
LISEVNNQIGYAERSFAYLDKELKSKQAQLDKADIEHKLALQNTLKETRDYYYLSLSDQLENYQWLADLGESNEAQIDAF
KLLIDQKIKFLSASLSFDASREQRLSDQLQHSSESEKSSLTLDHLFFERKVFNSTSGLEKLISIADQLDINTTDYKRQLF
STTGNLTEDILKFPVIAAILSNWISSGSNWLLKHAPDQLLKFLVFILILFATRLIARLTSRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIYFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVEAGSVSGKVSH
MSLVNTTIKTFDNQIIILPNSMIWGDVIKNVTHERVRRVDMVFGIGYEDSIEHAEKILAEIVDAHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTDDYWDVYWDITREVKLRFDKEGISIPFAQQDVHVHFAKKDKKPKKPNPITEKIGNVIDSVRSKD
S

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=277, Percent_Identity=32.4909747292419, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1786670, Length=252, Percent_Identity=26.5873015873016, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787591, Length=207, Percent_Identity=28.9855072463768, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI2367355, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 63689; Mature: 63689

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFIWILLFFSSLSFASEAQDLSHLEKINAEIARLKTESNSFSGSELAIIRVQQVEKNN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCH
ELRSVLSQLIQEHDKTTKELLISEVNNQIGYAERSFAYLDKELKSKQAQLDKADIEHKLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNTLKETRDYYYLSLSDQLENYQWLADLGESNEAQIDAFKLLIDQKIKFLSASLSFDAS
HHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
REQRLSDQLQHSSESEKSSLTLDHLFFERKVFNSTSGLEKLISIADQLDINTTDYKRQLF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHH
STTGNLTEDILKFPVIAAILSNWISSGSNWLLKHAPDQLLKFLVFILILFATRLIARLTS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLKAVVQPHLRMSKLMQDFFVSMSSKLIYFIGILIAFSKIGLDLAPVLTGFGVAGIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
GFALQDTLSNFASGMMLLIYRPFDVGDLVEAGSVSGKVSHMSLVNTTIKTFDNQIIILPN
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECC
SMIWGDVIKNVTHERVRRVDMVFGIGYEDSIEHAEKILAEIVDAHPAALKKPEPNIRVHT
CHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEE
LGASSVDFIVRPWVKTDDYWDVYWDITREVKLRFDKEGISIPFAQQDVHVHFAKKDKKPK
CCCCCCCHHEECCCCCCCCEEHHEEHEEHHHEEECCCCCCCCCCCCCCEEEEECCCCCCC
KPNPITEKIGNVIDSVRSKDS
CCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRHFIWILLFFSSLSFASEAQDLSHLEKINAEIARLKTESNSFSGSELAIIRVQQVEKNN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCH
ELRSVLSQLIQEHDKTTKELLISEVNNQIGYAERSFAYLDKELKSKQAQLDKADIEHKLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNTLKETRDYYYLSLSDQLENYQWLADLGESNEAQIDAFKLLIDQKIKFLSASLSFDAS
HHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
REQRLSDQLQHSSESEKSSLTLDHLFFERKVFNSTSGLEKLISIADQLDINTTDYKRQLF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHH
STTGNLTEDILKFPVIAAILSNWISSGSNWLLKHAPDQLLKFLVFILILFATRLIARLTS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLKAVVQPHLRMSKLMQDFFVSMSSKLIYFIGILIAFSKIGLDLAPVLTGFGVAGIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
GFALQDTLSNFASGMMLLIYRPFDVGDLVEAGSVSGKVSHMSLVNTTIKTFDNQIIILPN
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECC
SMIWGDVIKNVTHERVRRVDMVFGIGYEDSIEHAEKILAEIVDAHPAALKKPEPNIRVHT
CHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEE
LGASSVDFIVRPWVKTDDYWDVYWDITREVKLRFDKEGISIPFAQQDVHVHFAKKDKKPK
CCCCCCCHHEECCCCCCCCEEHHEEHEEHHHEEECCCCCCCCCCCCCCEEEEECCCCCCC
KPNPITEKIGNVIDSVRSKDS
CCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]