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The map label for this gene is ligA [H]

Identifier: 59712057

GI number: 59712057

Start: 1601052

End: 1601909

Strand: Reverse

Name: ligA [H]

Synonym: VF_1450

Alternate gene names: 59712057

Gene position: 1601909-1601052 (Counterclockwise)

Preceding gene: 59712059

Following gene: 59712050

Centisome position: 55.29

GC content: 39.16

Gene sequence:

>858_bases
ATGATGAAAATAAAATTACTTGCAGCCTTGGTTGCGCTTGGGTTAACCCCACTCCCCACATATTCACATGATGAACCAAA
TACTGCATCAATAGATGTGGTTCTTGCTACTCAATATGAAAAAGGAATGGATATTGAAGGGTATTGGGCAAGTGAGAAGT
TAGATGGTATTCGAGCATTATGGACAGGAGAAAAGTTAATAACACGAACAGGTCACACGATATATGCACCAGATTGGTTT
ACTGGCCCATTACCTGATCATAAGATTGATGGAGAGTTATGGGCTGGAAGAGGTGGATTTCAACAAGTTGCAAAAACAGT
GTTAGACAGTCAACCTAATGATGAATTATGGAAAAAAATTCGCTTCATGGCATTCGATTTACCTAGCTCTGCCGGTATGT
TTCCCAAACGATATTATGAGCTATCAACCATAGTTAAAAAGCTCGATCACCCGCACTTAAAATTGGTTGAACATTATCCT
ATCAGTAATGAAGACTCTCTATTAGAAGCATTAGATGATATCTCTGCACGTAATGGTGAAGGGGTGATGCTAAGAAAGGT
CAGTGAAGTTTATAGACCCGGACGCAGTGACGATTTAATTAAAGTGAAAAAACATCAAGACAATGAAGCCATTGTGATCG
GCTATACCGATGGGAAAGGAAAGTTTCGCGGTATGGTGGGCGCAATGATTTTACGTATGCCAAATGGAAAAGAATTTAAA
GTTGGTAGCGGTTTGAGTATTGAAGTAAGAGAAAACCCACCTAAATTAGGCTCTACAGTGACTTATCGTTATAACGGTTA
TACGGATAACGGAATCCCAAGATTTGCACGATACGTTGCAGTGAGAAATAATTATTAG

Upstream 100 bases:

>100_bases
GGAGATGGATCGAAATAGAGTGATATTTGTTCAATTTTTTCGTTAGTTGTTTGTGGGCTTATGTAATCATGAATTAAAGG
AATAATGATAATAGGTCAGG

Downstream 100 bases:

>100_bases
AATAGAAAGAGCCTCTTATTATCTTCCATTAGAAAGGAATATGATGAGAGGCTCTATTGTTCTGCTTTATTTTTTATTCA
GTGATGGTTCTAATTTGATA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MMKIKLLAALVALGLTPLPTYSHDEPNTASIDVVLATQYEKGMDIEGYWASEKLDGIRALWTGEKLITRTGHTIYAPDWF
TGPLPDHKIDGELWAGRGGFQQVAKTVLDSQPNDELWKKIRFMAFDLPSSAGMFPKRYYELSTIVKKLDHPHLKLVEHYP
ISNEDSLLEALDDISARNGEGVMLRKVSEVYRPGRSDDLIKVKKHQDNEAIVIGYTDGKGKFRGMVGAMILRMPNGKEFK
VGSGLSIEVRENPPKLGSTVTYRYNGYTDNGIPRFARYVAVRNNY

Sequences:

>Translated_285_residues
MMKIKLLAALVALGLTPLPTYSHDEPNTASIDVVLATQYEKGMDIEGYWASEKLDGIRALWTGEKLITRTGHTIYAPDWF
TGPLPDHKIDGELWAGRGGFQQVAKTVLDSQPNDELWKKIRFMAFDLPSSAGMFPKRYYELSTIVKKLDHPHLKLVEHYP
ISNEDSLLEALDDISARNGEGVMLRKVSEVYRPGRSDDLIKVKKHQDNEAIVIGYTDGKGKFRGMVGAMILRMPNGKEFK
VGSGLSIEVRENPPKLGSTVTYRYNGYTDNGIPRFARYVAVRNNY
>Mature_285_residues
MMKIKLLAALVALGLTPLPTYSHDEPNTASIDVVLATQYEKGMDIEGYWASEKLDGIRALWTGEKLITRTGHTIYAPDWF
TGPLPDHKIDGELWAGRGGFQQVAKTVLDSQPNDELWKKIRFMAFDLPSSAGMFPKRYYELSTIVKKLDHPHLKLVEHYP
ISNEDSLLEALDDISARNGEGVMLRKVSEVYRPGRSDDLIKVKKHQDNEAIVIGYTDGKGKFRGMVGAMILRMPNGKEFK
VGSGLSIEVRENPPKLGSTVTYRYNGYTDNGIPRFARYVAVRNNY

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 32051; Mature: 32051

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIKLLAALVALGLTPLPTYSHDEPNTASIDVVLATQYEKGMDIEGYWASEKLDGIRAL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCHHCCEEEE
WTGEKLITRTGHTIYAPDWFTGPLPDHKIDGELWAGRGGFQQVAKTVLDSQPNDELWKKI
ECCCHHEEECCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHH
RFMAFDLPSSAGMFPKRYYELSTIVKKLDHPHLKLVEHYPISNEDSLLEALDDISARNGE
HHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
GVMLRKVSEVYRPGRSDDLIKVKKHQDNEAIVIGYTDGKGKFRGMVGAMILRMPNGKEFK
CCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHEEHEEECCCCCEEE
VGSGLSIEVRENPPKLGSTVTYRYNGYTDNGIPRFARYVAVRNNY
ECCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEECCC
>Mature Secondary Structure
MMKIKLLAALVALGLTPLPTYSHDEPNTASIDVVLATQYEKGMDIEGYWASEKLDGIRAL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCHHCCEEEE
WTGEKLITRTGHTIYAPDWFTGPLPDHKIDGELWAGRGGFQQVAKTVLDSQPNDELWKKI
ECCCHHEEECCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHH
RFMAFDLPSSAGMFPKRYYELSTIVKKLDHPHLKLVEHYPISNEDSLLEALDDISARNGE
HHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
GVMLRKVSEVYRPGRSDDLIKVKKHQDNEAIVIGYTDGKGKFRGMVGAMILRMPNGKEFK
CCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHEEHEEECCCCCEEE
VGSGLSIEVRENPPKLGSTVTYRYNGYTDNGIPRFARYVAVRNNY
ECCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]