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The map label for this gene is potD1 [H]

Identifier: 59711926

GI number: 59711926

Start: 1467504

End: 1468547

Strand: Direct

Name: potD1 [H]

Synonym: VF_1319

Alternate gene names: 59711926

Gene position: 1467504-1468547 (Clockwise)

Preceding gene: 59711925

Following gene: 59711928

Centisome position: 50.65

GC content: 39.46

Gene sequence:

>1044_bases
ATGAAAAAGTGGACAACTCTCGTCTCTACAGGACTGTGCGCTGCAGCCTTAATCGCTGGTAATGCACAAGCTGCAGATAA
AGAGCTCTATTTTTATAACTGGTCAGAATACATTCCAAATGAAGTTCTTGAAGACTTCACTAAAGAAACTGGTATCAAAG
TTTACTATTCGACGTATGAGTCGAATGAAAGCATGTACGCTAAATTAAAAACTCAAGGTTCTGGATATGATCTAGTTGTT
CCTTCAACTTACTTTGTGTCGAAAATGCGCAAAGAAGGTATGCTACAAAAGATTGATAAATCTAAATTACCTCATTTCAA
AGACTTAGATCCAAACTACCTAAATAAGCCTTTTGATCCAAATAACAACTACTCTATTCCTTACATTTGGGGCGCAACAG
GTATCGGCGTAAATGCTGATATGATGAACCCGAATGAGCTTCATTCTTGGAACGATTTCTGGGATGAAAAATGGCAAGGT
CAGTTAATGATGATGGATGACTCACGTGAAGTGTTCCATATCGCATTAACTAAACTTGGTTACTCAGCAAATACAACAAA
TCCTGAAGAAATCAAAGCCGCTTACGAAGAACTGAAAAAACTAATGCCTAACATTTTGGTGTTTAACTCAGACTTCCCTG
CAAACCCATACTTAGCGGGTGAAACAAGTGTTGGTATGCTTTGGAACGGTTCTGCTTACATGGCTCGTGAAGAAGGTGCA
AACATTCAAATCGTATGGCCAGAAAAAGGCGCTATCTTCTGGATGGATAGCCTAGCAATTCCTGCTGGTGCAAAAAACAT
CGATGCTGCACACGAAATGATGGATTTCTTACTACGTCCTGAAAACGCCGCTAAAATTGCATTAGAAATCGGCTACCCAA
CACCAGTGAAATCAGCATACCCTCTTTTACCTAAAGAGTTTGCTAATGATCCAAACATCTTCCCTCCTCAATCTGTAATG
GATTCAGGTGAATGGCAAGACGAAGTTGGTGAAGCAAGTACGCTTTATGACGAATACTTCCAAAAGCTAAAAGTTGATAT
GTAA

Upstream 100 bases:

>100_bases
TAAGCCAAGTTATTGATTTGCAATGACATGCAGTCTCCTGCAAAAATTGCTATAATGCGCGCTCAATTATGGATACTCCC
CTCATATATAGGAGAAGGAA

Downstream 100 bases:

>100_bases
TACCCATCTATTACATCGTAATACAACACATTTAAAAGCCAATGAATCTAATGATTTATTGGCTTTTTTTATTTTTTAAA
AGTTATATTTCTAATAGTTC

Product: polyamine transporter binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEVLEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKSKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPNELHSWNDFWDEKWQG
QLMMMDDSREVFHIALTKLGYSANTTNPEEIKAAYEELKKLMPNILVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPEKGAIFWMDSLAIPAGAKNIDAAHEMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKEFANDPNIFPPQSVM
DSGEWQDEVGEASTLYDEYFQKLKVDM

Sequences:

>Translated_347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEVLEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKSKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPNELHSWNDFWDEKWQG
QLMMMDDSREVFHIALTKLGYSANTTNPEEIKAAYEELKKLMPNILVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPEKGAIFWMDSLAIPAGAKNIDAAHEMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKEFANDPNIFPPQSVM
DSGEWQDEVGEASTLYDEYFQKLKVDM
>Mature_347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEVLEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKSKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPNELHSWNDFWDEKWQG
QLMMMDDSREVFHIALTKLGYSANTTNPEEIKAAYEELKKLMPNILVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPEKGAIFWMDSLAIPAGAKNIDAAHEMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKEFANDPNIFPPQSVM
DSGEWQDEVGEASTLYDEYFQKLKVDM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=346, Percent_Identity=60.1156069364162, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787078, Length=340, Percent_Identity=37.0588235294118, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39357; Mature: 39357

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEVLEDFTKETGIKVYYSTYE
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCHHHHHHHHHHHCCCEEEEEEEC
SNESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKIDKSKLPHFKDLDPNYLNKPFDP
CCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
NNNYSIPYIWGATGIGVNADMMNPNELHSWNDFWDEKWQGQLMMMDDSREVFHIALTKLG
CCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHCCEEEEEECCCCEEEHHHHHHC
YSANTTNPEEIKAAYEELKKLMPNILVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCEEEEECCCC
NIQIVWPEKGAIFWMDSLAIPAGAKNIDAAHEMMDFLLRPENAAKIALEIGYPTPVKSAY
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
PLLPKEFANDPNIFPPQSVMDSGEWQDEVGEASTLYDEYFQKLKVDM
CCCCHHHCCCCCCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEVLEDFTKETGIKVYYSTYE
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCHHHHHHHHHHHCCCEEEEEEEC
SNESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKIDKSKLPHFKDLDPNYLNKPFDP
CCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
NNNYSIPYIWGATGIGVNADMMNPNELHSWNDFWDEKWQGQLMMMDDSREVFHIALTKLG
CCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHCCEEEEEECCCCEEEHHHHHHC
YSANTTNPEEIKAAYEELKKLMPNILVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCEEEEECCCC
NIQIVWPEKGAIFWMDSLAIPAGAKNIDAAHEMMDFLLRPENAAKIALEIGYPTPVKSAY
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
PLLPKEFANDPNIFPPQSVMDSGEWQDEVGEASTLYDEYFQKLKVDM
CCCCHHHCCCCCCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]