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The map label for this gene is potD2 [H]

Identifier: 59711925

GI number: 59711925

Start: 1466268

End: 1467302

Strand: Direct

Name: potD2 [H]

Synonym: VF_1318

Alternate gene names: 59711925

Gene position: 1466268-1467302 (Clockwise)

Preceding gene: 59711924

Following gene: 59711926

Centisome position: 50.6

GC content: 39.61

Gene sequence:

>1035_bases
ATGAACAAAATGGCTGCGTTTTTTACTGGAACCGCCTGCGCTTTAGCACTAACAATGAATGTTGCAAACGCAAAAGAAAA
TGATGAATTGGTATTCATGAACTGGGGACCATACATCAACAGTGATATTTTAGAAGAATTTACTAAAGAAACTGGCATTA
AAGTTATCTACTCTACTTATGAGTCAAATGAAACTTTATACGCTAAAATGAAGGCTCACAATAAAGGTTATGACCTAGTT
GTTCCTTCAACTTATTTCGTTGCAAAAATGCGTGACGAAAAAATGCTTCAACCAATTGATAAATCAAAACTATCAAACTT
CACTCAATTGGATACGAACTACTTAGACAAGCCATTTGATCCTAAGAATGAATACTCTATTCCACACGTAGTGGCGATCA
CAGGTCTTGCTGTAAACACAGAAATGTATAACCCAGATGACTTCAATAGCTGGGGCGATCTTTGGAACCCTGAGTTTGAA
GGTCAATTAATGTTAATGGATGATACTCGTGAAGTATTCCACATTGCATTACGTAAATTAGGTTACTCAGGTAACTCAAC
AAACCCGAAAGAAATTGACGAAGCGTATGCTGAGCTACAAAAACTAATGCCAAACGTATTAGTATTCAACTCAGACAACC
CAGGTGCACCATACATGGCTGGCGAAGTTGGTCTTGGTATGCTTTGGAATGGTTCTGCAGCCGCCGCTCAAGCTGAAGGT
TTACCTATTAAACTTATCTTCCCTAAAGAAGGTGGTATCGGTTGGGTTGATAACTTTGCAATCCCTTCAGGCGCAAACAA
TGTAGAAGCAGCTCATAAGATGATCGACTTTTTACTTCGTCCAGAAATCGCAGCACGCATTTCAGCAGATACTGGCTACC
TAACAGCAGTTAAAGCATCAAACGACAAGTTTAAAGACGTTGCACCTCTGTTCCCATCACAAGAAGATTTAGACCGCGTT
GAGTGGCAATCTGCAGTTGGTGACATGACAGTTAAATACGAAGAGTACTTCTTAAAACTGAAAGCCGGACAGTAA

Upstream 100 bases:

>100_bases
GTTTCATGCAAACCATCGTTTCATGTAAACAATAAATCTGGAAGACATGCCTTTTGTTCGACATGTCTTCTTTTTTATCA
CTCTAATGGAGAGTCAATCA

Downstream 100 bases:

>100_bases
CCTAACCCTTACTGAACCTTACTAATGGCAGCGTATATCGCTGCCATTTTTGATCTCCCTCGCACAAATTTCCTTACAAA
CTAATTTATATTACCTTGAT

Product: polyamine transporter binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNKMAAFFTGTACALALTMNVANAKENDELVFMNWGPYINSDILEEFTKETGIKVIYSTYESNETLYAKMKAHNKGYDLV
VPSTYFVAKMRDEKMLQPIDKSKLSNFTQLDTNYLDKPFDPKNEYSIPHVVAITGLAVNTEMYNPDDFNSWGDLWNPEFE
GQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPGAPYMAGEVGLGMLWNGSAAAAQAEG
LPIKLIFPKEGGIGWVDNFAIPSGANNVEAAHKMIDFLLRPEIAARISADTGYLTAVKASNDKFKDVAPLFPSQEDLDRV
EWQSAVGDMTVKYEEYFLKLKAGQ

Sequences:

>Translated_344_residues
MNKMAAFFTGTACALALTMNVANAKENDELVFMNWGPYINSDILEEFTKETGIKVIYSTYESNETLYAKMKAHNKGYDLV
VPSTYFVAKMRDEKMLQPIDKSKLSNFTQLDTNYLDKPFDPKNEYSIPHVVAITGLAVNTEMYNPDDFNSWGDLWNPEFE
GQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPGAPYMAGEVGLGMLWNGSAAAAQAEG
LPIKLIFPKEGGIGWVDNFAIPSGANNVEAAHKMIDFLLRPEIAARISADTGYLTAVKASNDKFKDVAPLFPSQEDLDRV
EWQSAVGDMTVKYEEYFLKLKAGQ
>Mature_344_residues
MNKMAAFFTGTACALALTMNVANAKENDELVFMNWGPYINSDILEEFTKETGIKVIYSTYESNETLYAKMKAHNKGYDLV
VPSTYFVAKMRDEKMLQPIDKSKLSNFTQLDTNYLDKPFDPKNEYSIPHVVAITGLAVNTEMYNPDDFNSWGDLWNPEFE
GQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPGAPYMAGEVGLGMLWNGSAAAAQAEG
LPIKLIFPKEGGIGWVDNFAIPSGANNVEAAHKMIDFLLRPEIAARISADTGYLTAVKASNDKFKDVAPLFPSQEDLDRV
EWQSAVGDMTVKYEEYFLKLKAGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=331, Percent_Identity=56.1933534743202, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787078, Length=314, Percent_Identity=32.8025477707006, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38406; Mature: 38406

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMAAFFTGTACALALTMNVANAKENDELVFMNWGPYINSDILEEFTKETGIKVIYSTY
CCCCEEEHHHHHEEEEEEEHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
ESNETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKSKLSNFTQLDTNYLDKPFD
CCCCEEEEEEECCCCCCEEEECCCEEEEECCHHHHHCCCCHHHHCCHHHCCHHHCCCCCC
PKNEYSIPHVVAITGLAVNTEMYNPDDFNSWGDLWNPEFEGQLMLMDDTREVFHIALRKL
CCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
GYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPGAPYMAGEVGLGMLWNGSAAAAQAEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEECCCCCCHHCCC
LPIKLIFPKEGGIGWVDNFAIPSGANNVEAAHKMIDFLLRPEIAARISADTGYLTAVKAS
CEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCEEEEEECC
NDKFKDVAPLFPSQEDLDRVEWQSAVGDMTVKYEEYFLKLKAGQ
CCCCCHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEECCC
>Mature Secondary Structure
MNKMAAFFTGTACALALTMNVANAKENDELVFMNWGPYINSDILEEFTKETGIKVIYSTY
CCCCEEEHHHHHEEEEEEEHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
ESNETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKSKLSNFTQLDTNYLDKPFD
CCCCEEEEEEECCCCCCEEEECCCEEEEECCHHHHHCCCCHHHHCCHHHCCHHHCCCCCC
PKNEYSIPHVVAITGLAVNTEMYNPDDFNSWGDLWNPEFEGQLMLMDDTREVFHIALRKL
CCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
GYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPGAPYMAGEVGLGMLWNGSAAAAQAEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEECCCCCCHHCCC
LPIKLIFPKEGGIGWVDNFAIPSGANNVEAAHKMIDFLLRPEIAARISADTGYLTAVKAS
CEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCEEEEEECC
NDKFKDVAPLFPSQEDLDRVEWQSAVGDMTVKYEEYFLKLKAGQ
CCCCCHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]