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The map label for this gene is allD [H]

Identifier: 59711859

GI number: 59711859

Start: 1392841

End: 1393788

Strand: Direct

Name: allD [H]

Synonym: VF_1252

Alternate gene names: 59711859

Gene position: 1392841-1393788 (Clockwise)

Preceding gene: 59711858

Following gene: 59711863

Centisome position: 48.07

GC content: 34.6

Gene sequence:

>948_bases
ATGAAAGTTTCATACTATGAAGTAAAAGAACGATTAATAAGAAAATTCATAGCTTCAGGATTGGCTTGGGATGATGCAAA
TTGGGTTACTGATGTTTTAATTTCTTCAGAGCAACGCGGTGATAAATCCCACGGGATAAAACATGCAAAGAATATATTTG
ATGTGATTAATAGTGAATGTTATATAGCACAGGCTCCTATTATTCATGATGAAAGGTCAATAACGATACTGGATGGACAG
AATTCTATAGGACCAATAGTAGCGAAACAGGCTATTGATATAGCAATAAAAAAAGCGAAGAAATATGGAACAGCAGCAAT
ATCTTTACGTTCATCAAATCATTTATTTTCATTAAGTCATTACGTTAGATATATAGCTAACAATAATATGATTGGTTTTA
TATGCAGTAGTAGCTCCCCCGCAATGGCTGCTCCAAACTCATTAAATGCCACAATTGGGACTAACCCATTTGCATTCGGA
GCACCATCATCAAAAGATCCCATAGTCATAGATATGAGTTCGACAAATGTTGCACGAGGAAAAATAAAAGAATATAAGGA
TGCGGAATTAGATATTCCAGTATCTTGGGCATTGGATGAATATGGAAATCCAACCACTTGCGCAATTGAGGCACTTAAAG
GCACTTTATCTCCATTGGGAGGTTATAAAGGTTTTGCTTTAGGCTGTATGATCGATATATTCAGTTCAGTATTGTCTGGT
TCTGCTTTTTCGACGCAGATTACAGGTACAAGTCTTCATATGGAAGAGGCTGATGTAAATAAGAAAGGAGATTTTTTATT
TGTCCTCGATATCAGCAAGTTTATTCAATTAAGTGAGTTTAAAATTCGTATGGATGAATTTATTCACATTATAGAAAGTA
ATGGTGGGTATATTCCAGGAACCAATTATATCAATAATCAGTTCGCTGATATAGAAATACTGAATTGA

Upstream 100 bases:

>100_bases
TTGTCTTGGTTTCACATCATTATTGGATTCGGACATTCCTAAACTAATGGCTGACATGATATATAACCGAATTTATCATG
AACATAATTAGGAGCGAAGA

Downstream 100 bases:

>100_bases
TGAGTGGGGACTGAACGATGAGAATTAACTTACTGGGTATAGTAAGAAATAGATCAAATAGTGGCATGATGATAAAAAGC
CGCTAATTAATAGCGGCTTT

Product: ureidoglycolate dehydrogenase

Products: oxaloacetate; NADH; H+

Alternate protein names: (R)-sulfolactate dehydrogenase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKVSYYEVKERLIRKFIASGLAWDDANWVTDVLISSEQRGDKSHGIKHAKNIFDVINSECYIAQAPIIHDERSITILDGQ
NSIGPIVAKQAIDIAIKKAKKYGTAAISLRSSNHLFSLSHYVRYIANNNMIGFICSSSSPAMAAPNSLNATIGTNPFAFG
APSSKDPIVIDMSSTNVARGKIKEYKDAELDIPVSWALDEYGNPTTCAIEALKGTLSPLGGYKGFALGCMIDIFSSVLSG
SAFSTQITGTSLHMEEADVNKKGDFLFVLDISKFIQLSEFKIRMDEFIHIIESNGGYIPGTNYINNQFADIEILN

Sequences:

>Translated_315_residues
MKVSYYEVKERLIRKFIASGLAWDDANWVTDVLISSEQRGDKSHGIKHAKNIFDVINSECYIAQAPIIHDERSITILDGQ
NSIGPIVAKQAIDIAIKKAKKYGTAAISLRSSNHLFSLSHYVRYIANNNMIGFICSSSSPAMAAPNSLNATIGTNPFAFG
APSSKDPIVIDMSSTNVARGKIKEYKDAELDIPVSWALDEYGNPTTCAIEALKGTLSPLGGYKGFALGCMIDIFSSVLSG
SAFSTQITGTSLHMEEADVNKKGDFLFVLDISKFIQLSEFKIRMDEFIHIIESNGGYIPGTNYINNQFADIEILN
>Mature_315_residues
MKVSYYEVKERLIRKFIASGLAWDDANWVTDVLISSEQRGDKSHGIKHAKNIFDVINSECYIAQAPIIHDERSITILDGQ
NSIGPIVAKQAIDIAIKKAKKYGTAAISLRSSNHLFSLSHYVRYIANNNMIGFICSSSSPAMAAPNSLNATIGTNPFAFG
APSSKDPIVIDMSSTNVARGKIKEYKDAELDIPVSWALDEYGNPTTCAIEALKGTLSPLGGYKGFALGCMIDIFSSVLSG
SAFSTQITGTSLHMEEADVNKKGDFLFVLDISKFIQLSEFKIRMDEFIHIIESNGGYIPGTNYINNQFADIEILN

Specific function: Catalyzes the reduction of sulfopyruvate to (R)- sulfolactate. Involved in the biosynthesis of both coenzyme M (with (R)-sulfolactate) and methanopterin (with alpha- ketoglutarate) [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786727, Length=254, Percent_Identity=31.8897637795276, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790000, Length=254, Percent_Identity=29.1338582677165, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787020, Length=234, Percent_Identity=26.9230769230769, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17507179, Length=295, Percent_Identity=29.8305084745763, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17536633, Length=301, Percent_Identity=29.2358803986711, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553211, Length=295, Percent_Identity=31.1864406779661, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667912, Length=295, Percent_Identity=31.1864406779661, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667908, Length=295, Percent_Identity=31.1864406779661, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667904, Length=295, Percent_Identity=31.1864406779661, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24658010, Length=289, Percent_Identity=28.0276816608997, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648360, Length=301, Percent_Identity=26.2458471760797, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: 1.1.1.37; 1.1.1.82

Molecular weight: Translated: 34467; Mature: 34467

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSYYEVKERLIRKFIASGLAWDDANWVTDVLISSEQRGDKSHGIKHAKNIFDVINSEC
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHHCCCE
YIAQAPIIHDERSITILDGQNSIGPIVAKQAIDIAIKKAKKYGTAAISLRSSNHLFSLSH
EEEECCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHH
YVRYIANNNMIGFICSSSSPAMAAPNSLNATIGTNPFAFGAPSSKDPIVIDMSSTNVARG
HHHHHHCCCEEEEEECCCCCCEECCCCCCEEECCCCEECCCCCCCCCEEEEECCCCCCCC
KIKEYKDAELDIPVSWALDEYGNPTTCAIEALKGTLSPLGGYKGFALGCMIDIFSSVLSG
HHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
SAFSTQITGTSLHMEEADVNKKGDFLFVLDISKFIQLSEFKIRMDEFIHIIESNGGYIPG
CCCEEEECCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHEEHHHEEEEEECCCCCCCC
TNYINNQFADIEILN
CCCCCCCCCEEEEEC
>Mature Secondary Structure
MKVSYYEVKERLIRKFIASGLAWDDANWVTDVLISSEQRGDKSHGIKHAKNIFDVINSEC
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHHCCCE
YIAQAPIIHDERSITILDGQNSIGPIVAKQAIDIAIKKAKKYGTAAISLRSSNHLFSLSH
EEEECCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHH
YVRYIANNNMIGFICSSSSPAMAAPNSLNATIGTNPFAFGAPSSKDPIVIDMSSTNVARG
HHHHHHCCCEEEEEECCCCCCEECCCCCCEEECCCCEECCCCCCCCCEEEEECCCCCCCC
KIKEYKDAELDIPVSWALDEYGNPTTCAIEALKGTLSPLGGYKGFALGCMIDIFSSVLSG
HHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
SAFSTQITGTSLHMEEADVNKKGDFLFVLDISKFIQLSEFKIRMDEFIHIIESNGGYIPG
CCCEEEECCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHEEHHHEEEEEECCCCCCCC
TNYINNQFADIEILN
CCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-malate; NAD+

Specific reaction: (S)-malate + NAD+ = oxaloacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]