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The map label for this gene is carB3 [H]

Identifier: 59711706

GI number: 59711706

Start: 1213593

End: 1214444

Strand: Reverse

Name: carB3 [H]

Synonym: VF_1099

Alternate gene names: 59711706

Gene position: 1214444-1213593 (Counterclockwise)

Preceding gene: 59711707

Following gene: 59711705

Centisome position: 41.91

GC content: 37.79

Gene sequence:

>852_bases
ATGAAAATCAAACCATTTCTATTTGGCCTTATTGTTTTAGCAAACAATGCAATTGCGGGCTCTCTTACTCAAAATATTGA
AGCTATCGAGCTATCAAGCGGAGGTTACATTGGGGTTTCAGTACTGAATACAGAAACAAACAAAACATGGGCATATAAAG
GCGACCAACGCTTTCCTATGATGAGTACATTTAAAACCCTTGCCTGTGCAAAAATGCTTTACGACTCAAGTGTCGGTGGC
ATTGATAAATACAAAACCACCTCTATAACTAAAGAACAAATCATGCCTTGGTCACCCGTTACAGAACCTTTAATCGGTAA
TATGATTACGACTCAAAAAGCGTGTGAAGCAACCATGCTAATGAGTGATAATACTGCTGCGAATATTGTTCTAAATGAAA
TTGGTGGGCCAAGTTCTTTAACGCAATTCTTGCGTGCTATGGGTGATACAAAAACTCGCCTTGATCGCATTGAACCAGAA
TTAAATGAATCTAAAAATGGCGATAAACGAGATACAACAACACCCGTTGCTATGTCGCAAACACTCAACGCTTTGTTATT
TGGAAACACTTTAAACTCACAGGATGAACAAACATTAAAATCTTGGATGATGAACAACAAAGTGTCTGATCCACTACTGC
GCTCTATTTTACCTAATGACTGGTCTATTGCTGATCGCTCAGGTGCTGGTGGTTTTGGTTCGCGTGGTATCACTGCCGCT
ATCTGGAACGACAAACACCAACCTATAATCATTAGTATTTACTTAACTCAAACAAAGCTTGATATGGCTGAACGTAATCA
AGTTATTGTTGAAGTTGGTAATGCTATTTTCAAAGAATTTAAAATTAATTAA

Upstream 100 bases:

>100_bases
TACTTGGGAATGAAAGTATGCTTCCAAGATACAAACGAAAGTTTGACTAATTTTAATATTAATATATATAGCTGTATAAA
AATTATCTTTAGGACTTCTG

Downstream 100 bases:

>100_bases
GAACAAAATTATGATCATTTTTACTACCAACTTTGGTGATATTCACATTGAGCTAAACAAAGAGAAAGCCCCTGTTAGCT
CAAAGAACTTTTTAAAATAT

Product: beta-lactamase

Products: NA

Alternate protein names: Carbenicillinase 3 [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKIKPFLFGLIVLANNAIAGSLTQNIEAIELSSGGYIGVSVLNTETNKTWAYKGDQRFPMMSTFKTLACAKMLYDSSVGG
IDKYKTTSITKEQIMPWSPVTEPLIGNMITTQKACEATMLMSDNTAANIVLNEIGGPSSLTQFLRAMGDTKTRLDRIEPE
LNESKNGDKRDTTTPVAMSQTLNALLFGNTLNSQDEQTLKSWMMNNKVSDPLLRSILPNDWSIADRSGAGGFGSRGITAA
IWNDKHQPIIISIYLTQTKLDMAERNQVIVEVGNAIFKEFKIN

Sequences:

>Translated_283_residues
MKIKPFLFGLIVLANNAIAGSLTQNIEAIELSSGGYIGVSVLNTETNKTWAYKGDQRFPMMSTFKTLACAKMLYDSSVGG
IDKYKTTSITKEQIMPWSPVTEPLIGNMITTQKACEATMLMSDNTAANIVLNEIGGPSSLTQFLRAMGDTKTRLDRIEPE
LNESKNGDKRDTTTPVAMSQTLNALLFGNTLNSQDEQTLKSWMMNNKVSDPLLRSILPNDWSIADRSGAGGFGSRGITAA
IWNDKHQPIIISIYLTQTKLDMAERNQVIVEVGNAIFKEFKIN
>Mature_283_residues
MKIKPFLFGLIVLANNAIAGSLTQNIEAIELSSGGYIGVSVLNTETNKTWAYKGDQRFPMMSTFKTLACAKMLYDSSVGG
IDKYKTTSITKEQIMPWSPVTEPLIGNMITTQKACEATMLMSDNTAANIVLNEIGGPSSLTQFLRAMGDTKTRLDRIEPE
LNESKNGDKRDTTTPVAMSQTLNALLFGNTLNSQDEQTLKSWMMNNKVSDPLLRSILPNDWSIADRSGAGGFGSRGITAA
IWNDKHQPIIISIYLTQTKLDMAERNQVIVEVGNAIFKEFKIN

Specific function: Hydrolyzes both carbenicillin and oxacillin [H]

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 31128; Mature: 31128

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKPFLFGLIVLANNAIAGSLTQNIEAIELSSGGYIGVSVLNTETNKTWAYKGDQRFPM
CCCCHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCEEEEEEEECCCCCEEEECCCCCCCH
MSTFKTLACAKMLYDSSVGGIDKYKTTSITKEQIMPWSPVTEPLIGNMITTQKACEATML
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCHHHHHHHHHEEEE
MSDNTAANIVLNEIGGPSSLTQFLRAMGDTKTRLDRIEPELNESKNGDKRDTTTPVAMSQ
ECCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
TLNALLFGNTLNSQDEQTLKSWMMNNKVSDPLLRSILPNDWSIADRSGAGGFGSRGITAA
HHHHEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IWNDKHQPIIISIYLTQTKLDMAERNQVIVEVGNAIFKEFKIN
EECCCCCCEEEEEEEECCHHHHHHCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MKIKPFLFGLIVLANNAIAGSLTQNIEAIELSSGGYIGVSVLNTETNKTWAYKGDQRFPM
CCCCHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCEEEEEEEECCCCCEEEECCCCCCCH
MSTFKTLACAKMLYDSSVGGIDKYKTTSITKEQIMPWSPVTEPLIGNMITTQKACEATML
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCHHHHHHHHHEEEE
MSDNTAANIVLNEIGGPSSLTQFLRAMGDTKTRLDRIEPELNESKNGDKRDTTTPVAMSQ
ECCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
TLNALLFGNTLNSQDEQTLKSWMMNNKVSDPLLRSILPNDWSIADRSGAGGFGSRGITAA
HHHHEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IWNDKHQPIIISIYLTQTKLDMAERNQVIVEVGNAIFKEFKIN
EECCCCCCEEEEEEEECCHHHHHHCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1650733 [H]