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The map label for this gene is rluB [H]

Identifier: 59711645

GI number: 59711645

Start: 1144323

End: 1145387

Strand: Direct

Name: rluB [H]

Synonym: VF_1038

Alternate gene names: 59711645

Gene position: 1144323-1145387 (Clockwise)

Preceding gene: 59711642

Following gene: 59711647

Centisome position: 39.49

GC content: 42.91

Gene sequence:

>1065_bases
ATGAGCGAAAAATTACAGAAAGTATTAGCACGAGCAGGTCTTGGTTCTCGTCGTGAGCTAGAAACCATGATTCAAGCTGG
TCGTGTTAGTGTTGATGGTCAGAGAGCAAAATTAGGCGACCGCCTAGAAAACGTGGATGCTAAAATCCGTATTGATGGCC
ATGTCGTATCGGTTAAAGAGTCAACTGATGTGATCTGTCGAGTACTTGCCTATCATAAACCTGAAGGTGAGCTATGTACA
CGTCATGACCCTGAAGGTCGTCGTACTGTATTTGATCGTTTACCAAAAATTCGTGATTCTCGTTGGGTATCAGTTGGCCG
TTTGGATGCTAATACCGCTGGTTTATTGCTTTTCACTACAGATGGTGAATTAGCAAACCGTTTGATGCACCCAAGTCGTA
AAGTTGAACGTGAATACATGTGTCGTGTATTTGGTGAAATTAACGAGAAAATGATTCGTAATCTAGTTACTGGTGTAAAA
CTAGAAGACGGTGAAGCGCGTTTTGAAGATGTAATGTACGCCGGCGGTGAAGGTATGAACCATACTTTCTACGTTGTTAT
TAATGAAGGCCGTAACCGTGAAGTACGTCGTTTATGGGATTCGCAAGGTGTAACGGTAAGTCGTCTAAAACGTGTTCGTT
ACGGTGATATCTTCCTTGAGAAAGCTTTACCACGTGGTGGTTGGATGGAGCTTGAACTTAAAGAAGTTAACTACCTACGT
GAGATGGTTGAATTACCGCCAGAGAAAGAAAGTCTACTTGATGTAGATAAAGAGTCTCGTAAGCGTGCTAAATCAAAAAC
TCAAAAGATCCGTCGTGCTGTTAAGCGTTATGATGAGCGTTCAACAGAGGAGCGTAATACTCCAGCGGGTCGTCGTGGCC
GCAACAATAAAAAACCAGTTGGTAGTTCACTGCCTTCAGCAAAACGTGGCTCTAATAATGGGGATAGCCAACCTTCTGGT
AAGCCACAATCTAAAGGTAAAAAACCAGCAGCTGGTCAAAAACCAGTAAGAAGTAAGTTTTCACCAAACGGTAAAGCGCC
AAAGTCTAAACCTAGACAGCGTTAA

Upstream 100 bases:

>100_bases
TAGAATTTGATAGTGATTAAGAGTGAAGGATTATCTATTCATCCTCTTGATTAATAAGAAGTTAATTGACGCCTGTGAAG
GCGACATAAAAAGGTAAAGA

Downstream 100 bases:

>100_bases
TTGCATCGTTAAAAACACTTAGATGAATAATAAAAGGGTTGTACCGTAAGGTAGCAACCCTTTTTTGTTCGTCTCTTTAA
AAATTTATTTTGGCTGAGCA

Product: 23S rRNA pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSEKLQKVLARAGLGSRRELETMIQAGRVSVDGQRAKLGDRLENVDAKIRIDGHVVSVKESTDVICRVLAYHKPEGELCT
RHDPEGRRTVFDRLPKIRDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRVFGEINEKMIRNLVTGVK
LEDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
EMVELPPEKESLLDVDKESRKRAKSKTQKIRRAVKRYDERSTEERNTPAGRRGRNNKKPVGSSLPSAKRGSNNGDSQPSG
KPQSKGKKPAAGQKPVRSKFSPNGKAPKSKPRQR

Sequences:

>Translated_354_residues
MSEKLQKVLARAGLGSRRELETMIQAGRVSVDGQRAKLGDRLENVDAKIRIDGHVVSVKESTDVICRVLAYHKPEGELCT
RHDPEGRRTVFDRLPKIRDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRVFGEINEKMIRNLVTGVK
LEDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
EMVELPPEKESLLDVDKESRKRAKSKTQKIRRAVKRYDERSTEERNTPAGRRGRNNKKPVGSSLPSAKRGSNNGDSQPSG
KPQSKGKKPAAGQKPVRSKFSPNGKAPKSKPRQR
>Mature_353_residues
SEKLQKVLARAGLGSRRELETMIQAGRVSVDGQRAKLGDRLENVDAKIRIDGHVVSVKESTDVICRVLAYHKPEGELCTR
HDPEGRRTVFDRLPKIRDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRVFGEINEKMIRNLVTGVKL
EDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLRE
MVELPPEKESLLDVDKESRKRAKSKTQKIRRAVKRYDERSTEERNTPAGRRGRNNKKPVGSSLPSAKRGSNNGDSQPSGK
PQSKGKKPAAGQKPVRSKFSPNGKAPKSKPRQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=299, Percent_Identity=65.5518394648829, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790453, Length=252, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 40188; Mature: 40056

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGLGSRRELETMIQAGRVSVDGQRAKLGDRLENVDAKIRIDGHVVSVKE
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEECC
STDVICRVLAYHKPEGELCTRHDPEGRRTVFDRLPKIRDSRWVSVGRLDANTAGLLLFTT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCEEEEECCCCCCCEEEEEEC
DGELANRLMHPSRKVEREYMCRVFGEINEKMIRNLVTGVKLEDGEARFEDVMYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
CEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EMVELPPEKESLLDVDKESRKRAKSKTQKIRRAVKRYDERSTEERNTPAGRRGRNNKKPV
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCC
GSSLPSAKRGSNNGDSQPSGKPQSKGKKPAAGQKPVRSKFSPNGKAPKSKPRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGLGSRRELETMIQAGRVSVDGQRAKLGDRLENVDAKIRIDGHVVSVKE
CHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEECC
STDVICRVLAYHKPEGELCTRHDPEGRRTVFDRLPKIRDSRWVSVGRLDANTAGLLLFTT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCEEEEECCCCCCCEEEEEEC
DGELANRLMHPSRKVEREYMCRVFGEINEKMIRNLVTGVKLEDGEARFEDVMYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
CEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EMVELPPEKESLLDVDKESRKRAKSKTQKIRRAVKRYDERSTEERNTPAGRRGRNNKKPV
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCC
GSSLPSAKRGSNNGDSQPSGKPQSKGKKPAAGQKPVRSKFSPNGKAPKSKPRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA