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The map label for this gene is luxO [H]

Identifier: 59711544

GI number: 59711544

Start: 1033510

End: 1034940

Strand: Direct

Name: luxO [H]

Synonym: VF_0937

Alternate gene names: 59711544

Gene position: 1033510-1034940 (Clockwise)

Preceding gene: 59711543

Following gene: 59711545

Centisome position: 35.67

GC content: 38.71

Gene sequence:

>1431_bases
ATGATACAAAAAAAATATTTATTAATGGTAGAAGATACTGCTTCCGTTGCTGCGTTATATCGCTCTTATTTAAACCCGTT
AGGTTTTGATATTGATGTCGTAGCTAAAGGTGTTGAAGCCATTGAGAAAATCAAATTAAGAACCCCTGATCTTGTGTTAC
TAGATTTGCGCCTGCCTGACATGACAGGTTTTGATGTGTTGGCTGAAATTCGTAAAGATAACCAAAGTATTCCTGTTGTA
TTAATGACGGCTCATGGCTCAATTGATGCTGCAGTAGAAGCCATGCAGTTAGGGGCTCAAGATTTCTTAATCAAACCATG
TGAAGCTGACCGTTTGAGGGTTACGGTAAATAACGCATTACGACGTGCACAAAAAGATCAGAAAGAATTCCAAGAGAATC
CAAAAGATACCAATAAACAGTATCAAGGATTTATTGGTAGTAGCTCTCAAATGCATCAGGTTTATCGCACCATAGATTCC
GCCGCACCAAGTAAAGCCACGGTATTTATTACTGGTGAATCGGGGACGGGTAAAGAGGTGTGTGCTGAAGCCATTCATGC
GGCTAGTAAGCGTGGAGACGGGCCTTTCATCGCTATTAACTGTGCGGCAATTCCAAAAGATTTAATTGAAAGTGAGCTTT
TTGGTCACGTTAAAGGCGCATTTACAGGTGCCTCTGTTGATCGAAAAGGTGCTGCCGAACAAGCAGATGGAGGTACGCTT
TTCCTCGATGAATTATGTGAAATGGATTTGGATTTACAGACCAAATTATTGCGATTTATTCAAACTGGTACTTTTCAAAA
AGTTGGTTCTTCTAAAATGAGCCGAGTTGATGTTCGATTTGTTTGTGCTACAAACCGAGACCCTTGGCTAGAAGTACAAG
CAGGCCGATTTAGAGAGGATTTATATTATCGTTTACATGTTATTCCTCTAACATTACCTCCTCTAAGAGACAGAGGAAAT
GATATTATTGAAATTGGTCACTCAATCCTTGGGCATTTTTCTCACGAGGAAGGTCGTGAGTTTATTACATTTAGTCCTGA
GGTTGTGGATAGGTTTTTGAATTACGATTGGCCTGGAAACGTACGACAATTACAAAATGTGATTAGGAATGTAGTCGTAT
TAAATAAAGGAAAAGAAGTCGAATTATCCATGCTGCCCCCTCCATTATCATTAGATAGTAAGAGTGGTGTAAATATTGCA
TCTAATGTATCCATAAGCGATTCAACTAAGCCTACAGAATCTATTCTGCCTAGTAATAGGATCTTAGATAGTAAAGATCA
AGAAACTATTATTCCGTTATGGCAATCTGAAAAACAGATCATTGAAAACGCAATTGAACTATGTGGTGGGAACATTCCAC
AAGCCGCTAAGCAATTAGAGGTTAGTCCTTCTACAATATATAGAAAACTACAGTCTTGGAATGAAAGATAA

Upstream 100 bases:

>100_bases
GCGCAAAGTCATCCTTTTTTGCATTTTGCAAATTGCGTTTTGCGAATCCTGATTGCATAATGCAAATATCAACCGCATTT
TTACCCTCAAGTTGGATTTG

Downstream 100 bases:

>100_bases
GGGGACTATCGTGAGTCAGTATGTTAATCAAATCGCTGTAAATCAATTAATGCAGGAAGTTGGAGATGAGAATATCCCTG
TGCTTTTTGGTATTTTTTGT

Product: autoinducer repressor protein LuxO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MIQKKYLLMVEDTASVAALYRSYLNPLGFDIDVVAKGVEAIEKIKLRTPDLVLLDLRLPDMTGFDVLAEIRKDNQSIPVV
LMTAHGSIDAAVEAMQLGAQDFLIKPCEADRLRVTVNNALRRAQKDQKEFQENPKDTNKQYQGFIGSSSQMHQVYRTIDS
AAPSKATVFITGESGTGKEVCAEAIHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTL
FLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMSRVDVRFVCATNRDPWLEVQAGRFREDLYYRLHVIPLTLPPLRDRGN
DIIEIGHSILGHFSHEEGREFITFSPEVVDRFLNYDWPGNVRQLQNVIRNVVVLNKGKEVELSMLPPPLSLDSKSGVNIA
SNVSISDSTKPTESILPSNRILDSKDQETIIPLWQSEKQIIENAIELCGGNIPQAAKQLEVSPSTIYRKLQSWNER

Sequences:

>Translated_476_residues
MIQKKYLLMVEDTASVAALYRSYLNPLGFDIDVVAKGVEAIEKIKLRTPDLVLLDLRLPDMTGFDVLAEIRKDNQSIPVV
LMTAHGSIDAAVEAMQLGAQDFLIKPCEADRLRVTVNNALRRAQKDQKEFQENPKDTNKQYQGFIGSSSQMHQVYRTIDS
AAPSKATVFITGESGTGKEVCAEAIHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTL
FLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMSRVDVRFVCATNRDPWLEVQAGRFREDLYYRLHVIPLTLPPLRDRGN
DIIEIGHSILGHFSHEEGREFITFSPEVVDRFLNYDWPGNVRQLQNVIRNVVVLNKGKEVELSMLPPPLSLDSKSGVNIA
SNVSISDSTKPTESILPSNRILDSKDQETIIPLWQSEKQIIENAIELCGGNIPQAAKQLEVSPSTIYRKLQSWNER
>Mature_476_residues
MIQKKYLLMVEDTASVAALYRSYLNPLGFDIDVVAKGVEAIEKIKLRTPDLVLLDLRLPDMTGFDVLAEIRKDNQSIPVV
LMTAHGSIDAAVEAMQLGAQDFLIKPCEADRLRVTVNNALRRAQKDQKEFQENPKDTNKQYQGFIGSSSQMHQVYRTIDS
AAPSKATVFITGESGTGKEVCAEAIHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTL
FLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMSRVDVRFVCATNRDPWLEVQAGRFREDLYYRLHVIPLTLPPLRDRGN
DIIEIGHSILGHFSHEEGREFITFSPEVVDRFLNYDWPGNVRQLQNVIRNVVVLNKGKEVELSMLPPPLSLDSKSGVNIA
SNVSISDSTKPTESILPSNRILDSKDQETIIPLWQSEKQIIENAIELCGGNIPQAAKQLEVSPSTIYRKLQSWNER

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=464, Percent_Identity=40.0862068965517, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=39.3162393162393, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790299, Length=480, Percent_Identity=39.375, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788905, Length=371, Percent_Identity=40.4312668463612, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=41.9161676646707, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082117, Length=301, Percent_Identity=43.8538205980066, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI87082152, Length=245, Percent_Identity=48.5714285714286, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1789087, Length=293, Percent_Identity=41.6382252559727, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1787583, Length=261, Percent_Identity=40.2298850574713, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=234, Percent_Identity=44.017094017094, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786524, Length=346, Percent_Identity=37.2832369942197, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=234, Percent_Identity=37.1794871794872, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=27.9742765273312, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=34.1880341880342, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789402, Length=116, Percent_Identity=32.7586206896552, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787487, Length=151, Percent_Identity=30.4635761589404, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790860, Length=124, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=30.7086614173228, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=145, Percent_Identity=26.8965517241379, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52910; Mature: 52910

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKKYLLMVEDTASVAALYRSYLNPLGFDIDVVAKGVEAIEKIKLRTPDLVLLDLRLPD
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
MTGFDVLAEIRKDNQSIPVVLMTAHGSIDAAVEAMQLGAQDFLIKPCEADRLRVTVNNAL
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHEECCCCCCCEEEEHHHHH
RRAQKDQKEFQENPKDTNKQYQGFIGSSSQMHQVYRTIDSAAPSKATVFITGESGTGKEV
HHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
CAEAIHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMSRVDVRFVCATNRDPWLEVQAGRFRED
EHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCCEEEECCCHHHHC
LYYRLHVIPLTLPPLRDRGNDIIEIGHSILGHFSHEEGREFITFSPEVVDRFLNYDWPGN
EEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCC
VRQLQNVIRNVVVLNKGKEVELSMLPPPLSLDSKSGVNIASNVSISDSTKPTESILPSNR
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCC
ILDSKDQETIIPLWQSEKQIIENAIELCGGNIPQAAKQLEVSPSTIYRKLQSWNER
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIQKKYLLMVEDTASVAALYRSYLNPLGFDIDVVAKGVEAIEKIKLRTPDLVLLDLRLPD
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
MTGFDVLAEIRKDNQSIPVVLMTAHGSIDAAVEAMQLGAQDFLIKPCEADRLRVTVNNAL
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHEECCCCCCCEEEEHHHHH
RRAQKDQKEFQENPKDTNKQYQGFIGSSSQMHQVYRTIDSAAPSKATVFITGESGTGKEV
HHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
CAEAIHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMSRVDVRFVCATNRDPWLEVQAGRFRED
EHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCCEEEECCCHHHHC
LYYRLHVIPLTLPPLRDRGNDIIEIGHSILGHFSHEEGREFITFSPEVVDRFLNYDWPGN
EEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCC
VRQLQNVIRNVVVLNKGKEVELSMLPPPLSLDSKSGVNIASNVSISDSTKPTESILPSNR
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCC
ILDSKDQETIIPLWQSEKQIIENAIELCGGNIPQAAKQLEVSPSTIYRKLQSWNER
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]