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The map label for this gene is ushA [H]

Identifier: 59711385

GI number: 59711385

Start: 849809

End: 851467

Strand: Direct

Name: ushA [H]

Synonym: VF_0778

Alternate gene names: 59711385

Gene position: 849809-851467 (Clockwise)

Preceding gene: 59711379

Following gene: 59711389

Centisome position: 29.33

GC content: 38.28

Gene sequence:

>1659_bases
ATGGAACGTCTTTCTCTAAAAACAGCTATTGCAGCTGCATTGGTAACAACTTTAGCTGGTTGTGCGACAACGTCTGAACC
TGCTTGGCAAGAAGATACAACATATAAAATCACAGTATTGCATACCAATGATAACCATGGTCGTTTTTGGCAGAACAAAT
ATGGTGAGTATGGAATGGCTGCTCGTAAAACGTTGATCGATGACATTCGAACTGAAGTGCAAGCAGAAGGGGGCAGTGTG
CTTCTTTTATCTGGTGGTGATATTAACACTGGTGTACCAGAATCAGATTTACAAGATGCAGAACCTGATTTCAAAGGCAT
GAATAAAATTGGTTATGATGCGATTGCATTAGGTAACCACGAATTTGATAATCCACTGGATGTACTGCAAAAACAAATTG
ATTGGGCTGAGTTCCCAATGTTATCTGCCAATATCTATGATAAAAAAACTGGCGAACGTATGTATCAAGCATACGAAATC
TTTGAAAAACAAGGTATTAAAATCGCTGTAGTTGGTTTAACAACAGAAGATACAGCAAAAATTGGTAACCCTGAGTACAT
CGGTAGTCTTGAATTCCGCGATCCTAAATTGGAAGCAAAGAAAGTGATTGCTGAATTAAATGAAACTGAAAAACCTGACC
TTATTTTTGCCGTAACTCATATGGGACACTATGAAAATGGTGAGCGTGGTATTAATGCTCCAGGTGATGTTGCTCTAGCG
CGTTCTTTGGATATGGGTGAGTTAGATATGATTGTGGGTGGTCACTCGCAAGAGCCTGTATGTATGGAAGGCCCTAACTT
AATTAAGAAAAACTTTAAGCCGGGTGATGAGTGTAAACCAGACCAGCAAAATGGTACATGGATTGTTCAAGCTCACGAAT
GGGGTAAATACGTAGGTAAAGCTGATTTTGAGTTCCGTAATGGTGAATTAGAAATGGTTAGCTATGATCTTATTCCTGTT
AACTTGAAGAAAAAAGTAAATATCGATGGTAAAAAACAACGTGTATTTATTGAAGATGAAATTGAACAAGATGCAGAGTT
ACTTGCTTTCTTAAAACCGTTCCAAGAAAAAGGACAAGGCCAACTTAATGTTAAGATTGCTTCAACTAATGGCAAATTAG
AAGGTGATCGTAATGTTGTTCGTTTTGAACAAACTAACTTAGGTCGCTTGATTGCTACATCACATATGGTTCGTGCAAAA
GCTGATTTTGCAGTAATGAACTCTGGTGGTGTACGTGATTCAATTGAAGCGGGTGATGTAACTTACAAAGACGTATTAAC
GGTTCAACCATTTGGCAATATCGTAACTTATGCGGATATGACGGGTAAAGAAGTGTTAGATTACCTTAACGTAGTTGCGA
CTAAGCCTGTTGATTCTGGTGCCTATGCTCAATTTGCTGGCATTAAAATGACTGTTGAAAATGGTAAAGTATCAAATGTA
TTTATTGGTGGTAAGCAATTACGCCTAGATGAAACATATCGTTTTACAGTACCAAGCTATAACGCTGCTGGTGGCGATGG
ATATCCAAAAATTTCAGATCACCCAGGCTATGTAAATACTGGTTTCGTTGATGCTGAAGTGTTGAAAGATTTCCTAGAAA
CAAACAGCCCAATCGACGTAAATACATTTGCTCCGCGTGGTGAAATTGTTTACAAATAA

Upstream 100 bases:

>100_bases
GACGTAATTCTTTTAAACTTGGTCAGGTTATTTGACCAGTTAAAGAGATAACATTCTTACTGGTTATAATTTATCCCCCT
TTGAATAATGGTGAAACATG

Downstream 100 bases:

>100_bases
CAGTAAGTTTACTGGTTAATAAAAAGCCTCCAGATATTTGGAGGCTTTTTTTATGCACTGCGTTTTTGATGCTTTTCTCT
GTTCTCTAATTTCTTTTCTG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MERLSLKTAIAAALVTTLAGCATTSEPAWQEDTTYKITVLHTNDNHGRFWQNKYGEYGMAARKTLIDDIRTEVQAEGGSV
LLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAEFPMLSANIYDKKTGERMYQAYEI
FEKQGIKIAVVGLTTEDTAKIGNPEYIGSLEFRDPKLEAKKVIAELNETEKPDLIFAVTHMGHYENGERGINAPGDVALA
RSLDMGELDMIVGGHSQEPVCMEGPNLIKKNFKPGDECKPDQQNGTWIVQAHEWGKYVGKADFEFRNGELEMVSYDLIPV
NLKKKVNIDGKKQRVFIEDEIEQDAELLAFLKPFQEKGQGQLNVKIASTNGKLEGDRNVVRFEQTNLGRLIATSHMVRAK
ADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFGNIVTYADMTGKEVLDYLNVVATKPVDSGAYAQFAGIKMTVENGKVSNV
FIGGKQLRLDETYRFTVPSYNAAGGDGYPKISDHPGYVNTGFVDAEVLKDFLETNSPIDVNTFAPRGEIVYK

Sequences:

>Translated_552_residues
MERLSLKTAIAAALVTTLAGCATTSEPAWQEDTTYKITVLHTNDNHGRFWQNKYGEYGMAARKTLIDDIRTEVQAEGGSV
LLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAEFPMLSANIYDKKTGERMYQAYEI
FEKQGIKIAVVGLTTEDTAKIGNPEYIGSLEFRDPKLEAKKVIAELNETEKPDLIFAVTHMGHYENGERGINAPGDVALA
RSLDMGELDMIVGGHSQEPVCMEGPNLIKKNFKPGDECKPDQQNGTWIVQAHEWGKYVGKADFEFRNGELEMVSYDLIPV
NLKKKVNIDGKKQRVFIEDEIEQDAELLAFLKPFQEKGQGQLNVKIASTNGKLEGDRNVVRFEQTNLGRLIATSHMVRAK
ADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFGNIVTYADMTGKEVLDYLNVVATKPVDSGAYAQFAGIKMTVENGKVSNV
FIGGKQLRLDETYRFTVPSYNAAGGDGYPKISDHPGYVNTGFVDAEVLKDFLETNSPIDVNTFAPRGEIVYK
>Mature_552_residues
MERLSLKTAIAAALVTTLAGCATTSEPAWQEDTTYKITVLHTNDNHGRFWQNKYGEYGMAARKTLIDDIRTEVQAEGGSV
LLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAEFPMLSANIYDKKTGERMYQAYEI
FEKQGIKIAVVGLTTEDTAKIGNPEYIGSLEFRDPKLEAKKVIAELNETEKPDLIFAVTHMGHYENGERGINAPGDVALA
RSLDMGELDMIVGGHSQEPVCMEGPNLIKKNFKPGDECKPDQQNGTWIVQAHEWGKYVGKADFEFRNGELEMVSYDLIPV
NLKKKVNIDGKKQRVFIEDEIEQDAELLAFLKPFQEKGQGQLNVKIASTNGKLEGDRNVVRFEQTNLGRLIATSHMVRAK
ADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFGNIVTYADMTGKEVLDYLNVVATKPVDSGAYAQFAGIKMTVENGKVSNV
FIGGKQLRLDETYRFTVPSYNAAGGDGYPKISDHPGYVNTGFVDAEVLKDFLETNSPIDVNTFAPRGEIVYK

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=545, Percent_Identity=22.9357798165138, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786687, Length=548, Percent_Identity=62.956204379562, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=491, Percent_Identity=21.5885947046843, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922446, Length=512, Percent_Identity=27.5390625, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24654424, Length=512, Percent_Identity=27.5390625, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922444, Length=549, Percent_Identity=24.9544626593807, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28573524, Length=529, Percent_Identity=25.5198487712665, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24641187, Length=550, Percent_Identity=22.5454545454545, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076508, Length=516, Percent_Identity=25.968992248062, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652512, Length=515, Percent_Identity=25.631067961165, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19921980, Length=515, Percent_Identity=25.631067961165, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI221329836, Length=540, Percent_Identity=22.962962962963, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 60985; Mature: 60985

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLSLKTAIAAALVTTLAGCATTSEPAWQEDTTYKITVLHTNDNHGRFWQNKYGEYGMA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCCCCCHH
ARKTLIDDIRTEVQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNH
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
EFDNPLDVLQKQIDWAEFPMLSANIYDKKTGERMYQAYEIFEKQGIKIAVVGLTTEDTAK
CCCCHHHHHHHHCCHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHC
IGNPEYIGSLEFRDPKLEAKKVIAELNETEKPDLIFAVTHMGHYENGERGINAPGDVALA
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
RSLDMGELDMIVGGHSQEPVCMEGPNLIKKNFKPGDECKPDQQNGTWIVQAHEWGKYVGK
ECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
ADFEFRNGELEMVSYDLIPVNLKKKVNIDGKKQRVFIEDEIEQDAELLAFLKPFQEKGQG
CCEEEECCEEEEEEEEEEEECCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
QLNVKIASTNGKLEGDRNVVRFEQTNLGRLIATSHMVRAKADFAVMNSGGVRDSIEAGDV
EEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEECCCCCCCCCCCCCE
TYKDVLTVQPFGNIVTYADMTGKEVLDYLNVVATKPVDSGAYAQFAGIKMTVENGKVSNV
EHHHEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEE
FIGGKQLRLDETYRFTVPSYNAAGGDGYPKISDHPGYVNTGFVDAEVLKDFLETNSPIDV
EECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
NTFAPRGEIVYK
EECCCCCCEEEC
>Mature Secondary Structure
MERLSLKTAIAAALVTTLAGCATTSEPAWQEDTTYKITVLHTNDNHGRFWQNKYGEYGMA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCCCCCHH
ARKTLIDDIRTEVQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNH
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
EFDNPLDVLQKQIDWAEFPMLSANIYDKKTGERMYQAYEIFEKQGIKIAVVGLTTEDTAK
CCCCHHHHHHHHCCHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHC
IGNPEYIGSLEFRDPKLEAKKVIAELNETEKPDLIFAVTHMGHYENGERGINAPGDVALA
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
RSLDMGELDMIVGGHSQEPVCMEGPNLIKKNFKPGDECKPDQQNGTWIVQAHEWGKYVGK
ECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
ADFEFRNGELEMVSYDLIPVNLKKKVNIDGKKQRVFIEDEIEQDAELLAFLKPFQEKGQG
CCEEEECCEEEEEEEEEEEECCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
QLNVKIASTNGKLEGDRNVVRFEQTNLGRLIATSHMVRAKADFAVMNSGGVRDSIEAGDV
EEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEECCCCCCCCCCCCCE
TYKDVLTVQPFGNIVTYADMTGKEVLDYLNVVATKPVDSGAYAQFAGIKMTVENGKVSNV
EHHHEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEE
FIGGKQLRLDETYRFTVPSYNAAGGDGYPKISDHPGYVNTGFVDAEVLKDFLETNSPIDV
EECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
NTFAPRGEIVYK
EECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA