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The map label for this gene is glyA [H]

Identifier: 59711302

GI number: 59711302

Start: 758768

End: 760018

Strand: Direct

Name: glyA [H]

Synonym: VF_0695

Alternate gene names: 59711302

Gene position: 758768-760018 (Clockwise)

Preceding gene: 59711300

Following gene: 59711303

Centisome position: 26.19

GC content: 42.05

Gene sequence:

>1251_bases
ATGCTTAAACGTGATATGAACATTGCCGATTACGATGCAGACTTATTTGCAGCAATCCAGGAAGAAACAGTACGTCAAGA
AGAGCATATCGAGCTGATTGCTTCTGAAAACTACACTAGCCCACGAGTAATGGAAGCTCAAGGTTCACAATTAACGAACA
AATATGCTGAAGGTTACCCTGGCAAACGTTACTACGGTGGTTGTGAGTACGTAGATAAAGTTGAAACACTTGCTATTGAA
CGTGCATGTGAGTTGTTTGGTGCAGAGTACGCAAACGTACAGCCTCACTCTGGTTCACAAGCAAATAACGCAGTTTACAT
GGCACTACTAAATGCGGGTGATACTGTATTAGGTATGAGCCTTGCTCACGGTGGTCACTTAACTCACGGTTCTCCAGTAA
ACTTCTCTGGTAAACTTTACAACATCATTCCTTACGGTATTGATGAAAATGGTCAAATTGACTATGAAGAGATGGAAGCA
TTAGCAGTAGAACATAAGCCTAAGATGATCATTGGTGGTTTCTCTGCTTACTCTCAAGTATGTGATTGGGCACGTATGCG
TGAAATTGCAGACAAAGTAGGCGCTTACTTCTTCGTAGATATGGCTCACGTAGCAGGTCTTATTGCTGCAGGTGTTTACC
CTAACCCTGTGCCACATGCTCACGTTGTAACAACAACGACACACAAAACACTTGCTGGTCCTCGTGGTGGTCTTATCCTT
TCTAATGAAGGTGAAGATCTTTACAAGAAGTTAAACTCAGCAGTATTCCCGGGTGGTCAAGGTGGTCCTTTAATGCACGT
TATTGCTGGTAAAGCAGTTGCATTTAAAGAAGCGCTTGAACCTGAGTTCAAAGAATATCAAGCACGTGTTGTTGCTAATG
CAAAAGCAATGGTTGCTGAATTCTTAGCTCGTGGTTACAACATCGTTTCAGGTTCAACAGAGAACCACCTGTTCCTAGTT
GATTTAATTGATAAAGATATTACAGGTAAAGAAGCGGATGCTGCACTAGGTTCTGCTAACATTACAGTAAACAAGAACTC
AGTACCGAATGACCCTCGTAGCCCGTTTGTTACTTCTGGTATTCGTATTGGTTCTCCTTCAATTACGCGTCGTGGTTTTA
CTGAAGAAGATGCGAAAAACCTTGCAGGCTGGATGTGTGACATTCTAGATAACATGGGTGATGAGTCTGTGATTGAAGCG
ACAAAAGCAAAAGTATTAGAAATCTGTAAGCGTCTACCAGTTTACGCTTAA

Upstream 100 bases:

>100_bases
CAAATTATGTAGAATACATTGCAGTTAAGACTGGTGACGTAAACGTTTGCGTCATTGGGCTATTTTTGGGTTTTATATTT
ATCTGAGTCAGGAGATACAG

Downstream 100 bases:

>100_bases
TTTTTTACTTTATTAAAATGTTAAAGGCTAGCCTGTAATGGGCTAGCCTTTTTTATTGATAGTTTTAACTATTTCTTTTT
TATACGATTATTTTGTGTTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1 [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLKRDMNIADYDADLFAAIQEETVRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDKVETLAIE
RACELFGAEYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDENGQIDYEEMEA
LAVEHKPKMIIGGFSAYSQVCDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFTEEDAKNLAGWMCDILDNMGDESVIEA
TKAKVLEICKRLPVYA

Sequences:

>Translated_416_residues
MLKRDMNIADYDADLFAAIQEETVRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDKVETLAIE
RACELFGAEYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDENGQIDYEEMEA
LAVEHKPKMIIGGFSAYSQVCDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFTEEDAKNLAGWMCDILDNMGDESVIEA
TKAKVLEICKRLPVYA
>Mature_416_residues
MLKRDMNIADYDADLFAAIQEETVRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDKVETLAIE
RACELFGAEYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDENGQIDYEEMEA
LAVEHKPKMIIGGFSAYSQVCDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFTEEDAKNLAGWMCDILDNMGDESVIEA
TKAKVLEICKRLPVYA

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=45.273631840796, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=45.273631840796, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=45.273631840796, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=45.273631840796, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=44.3890274314214, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=45.8852867830424, Blast_Score=318, Evalue=4e-87,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=43.298969072165, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=81.294964028777, Blast_Score=690, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=44.3890274314214, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=44.3890274314214, Blast_Score=337, Evalue=9e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=428, Percent_Identity=43.9252336448598, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=44.2786069651741, Blast_Score=338, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=45.5, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=45.5, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45177; Mature: 45177

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRDMNIADYDADLFAAIQEETVRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDKVETLAIERACELFGAEYANVQPHSGSQANNAVYMALLNAGDTVLGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDENGQIDYEEMEALAVEHKPKMIIGGFSAYSQV
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
CDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAE
ECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FLARGYNIVSGSTENHLFLVDLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEECC
IRIGSPSITRRGFTEEDAKNLAGWMCDILDNMGDESVIEATKAKVLEICKRLPVYA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKRDMNIADYDADLFAAIQEETVRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDKVETLAIERACELFGAEYANVQPHSGSQANNAVYMALLNAGDTVLGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDENGQIDYEEMEALAVEHKPKMIIGGFSAYSQV
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
CDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAE
ECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FLARGYNIVSGSTENHLFLVDLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEECC
IRIGSPSITRRGFTEEDAKNLAGWMCDILDNMGDESVIEATKAKVLEICKRLPVYA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA