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The map label for this gene is iscA [H]

Identifier: 59711226

GI number: 59711226

Start: 676335

End: 676658

Strand: Direct

Name: iscA [H]

Synonym: VF_0619

Alternate gene names: 59711226

Gene position: 676335-676658 (Clockwise)

Preceding gene: 59711225

Following gene: 59711227

Centisome position: 23.34

GC content: 39.81

Gene sequence:

>324_bases
ATGGCCATTTCAGTCACCGAAGCAGCGGCAAGCCGTGTACAAAAATTTCTTGAAAACCGAGGAAAAGGCGTTGGTTTACG
TTTAGGCGTAAGAACCTCTGGTTGTTCGGGTATGGCTTATATTCTTGAATTTGTTGATGAGCTAAACGAAGAAGATCAAG
TTTTCGAACAGTTTGGTGTAAAAATCATTATCGATCCAAAAAGCTTAGCTTATATGGATGGCACTGAATTGGACTTTGCA
AAAGAAGGTCTAAATGAAGGCTTTAAATTCAACAACCCGAACGTAAAAAGTGAATGTGGGTGTGGTGAAAGCTTCAACGT
ATAG

Upstream 100 bases:

>100_bases
TTTATTATTTGTTTTATTAATAAAAGGTGCTTTATTATTGAGTAATGTCGTTAATAATTAATGACTCCCATGACCACAAA
CAAAAAGTAAAGGTTGCAGT

Downstream 100 bases:

>100_bases
AGCAAAACATTACTTATGAATCATTTTGAATTATTTGGGCTACCGAACCAGTTTGAACTGGATGGTGGCCTTCTTTCTCT
TCAATTTAGAGAGTTGCAAA

Product: Fe-S cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAISVTEAAASRVQKFLENRGKGVGLRLGVRTSGCSGMAYILEFVDELNEEDQVFEQFGVKIIIDPKSLAYMDGTELDFA
KEGLNEGFKFNNPNVKSECGCGESFNV

Sequences:

>Translated_107_residues
MAISVTEAAASRVQKFLENRGKGVGLRLGVRTSGCSGMAYILEFVDELNEEDQVFEQFGVKIIIDPKSLAYMDGTELDFA
KEGLNEGFKFNNPNVKSECGCGESFNV
>Mature_106_residues
AISVTEAAASRVQKFLENRGKGVGLRLGVRTSGCSGMAYILEFVDELNEEDQVFEQFGVKIIIDPKSLAYMDGTELDFAK
EGLNEGFKFNNPNVKSECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=39.622641509434, Blast_Score=102, Evalue=7e-23,
Organism=Homo sapiens, GI160420328, Length=96, Percent_Identity=38.5416666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=71.9626168224299, Blast_Score=166, Evalue=4e-43,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=43.8095238095238, Blast_Score=109, Evalue=4e-26,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=40, Blast_Score=81, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=38.6792452830189, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=38.6792452830189, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=34.9056603773585, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323001, Length=82, Percent_Identity=47.5609756097561, Blast_Score=85, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=42.4528301886792, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11707; Mature: 11576

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISVTEAAASRVQKFLENRGKGVGLRLGVRTSGCSGMAYILEFVDELNEEDQVFEQFGV
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCC
KIIIDPKSLAYMDGTELDFAKEGLNEGFKFNNPNVKSECGCGESFNV
EEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISVTEAAASRVQKFLENRGKGVGLRLGVRTSGCSGMAYILEFVDELNEEDQVFEQFGV
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCC
KIIIDPKSLAYMDGTELDFAKEGLNEGFKFNNPNVKSECGCGESFNV
EEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12775707 [H]