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The map label for this gene is slt [H]

Identifier: 59711165

GI number: 59711165

Start: 606364

End: 608298

Strand: Direct

Name: slt [H]

Synonym: VF_0558

Alternate gene names: 59711165

Gene position: 606364-608298 (Clockwise)

Preceding gene: 59711161

Following gene: 59711166

Centisome position: 20.93

GC content: 36.38

Gene sequence:

>1935_bases
ATGAAATATACACAATATAAGTTAGGGATGATGACGGCATTGTGCATAGGTATGAATGTACAAGTTGTTGATGCGTCACC
TGTTGATACATGGCGAGCACAATACCAAGAAGCAAAAACGCTACTTAATGAAAAAAAATACGATGAATATAAAAAAGTAA
GGGCTGAACTTGATGGTTATTCATTAGCACCTTATTTGGATTACCGTGAATTAAGAATGGATTTAAGCTCTAAAACGCCT
GATGATATTCGTTTGTTCAAACATAATCATCAGTCATTACCTATTGGTAATTCTCTTTCATACCAATACTTAGTCATTCT
TGGTTTAAACGAGCGTTGGGCTGAATTTATTGATTATTTCCCCAACGAACCAAGCAGCAAAAACCTACAGTGTTATTACT
ATCAAGCAAAAAATAAACTGGGTGATAAAGCCACAGCTTGGAAAGGTGCGGAGCAATTATGGTTAACAGGTAGCTCTGTT
ACTTATGAATGTGATGATTTATTTCAAGATTGGGCTGAAGCTGGGAAACTCAGTGATGATTTAATTATTGAACGTATGCT
TTTAGTTTATAAAGCTCGTAATAATAATTTGTTTTCTTATTTAGAAAAAATGTTGAAAGCAGATGCCGCAAAAGAAAAAG
CACAGCATATTGTCACTTTGTTTAATGATCCTGATTTGTTAGAAAGTTTTTCAAAGAGTAATAAAAAAACAGAGTTTTCT
AAGCAATTAACGCTTATTTCTATGGAAAGAGAAGCTCGTAAAAACCCTAAAAAAGCGATAGAAATATTGCCTGACATTGC
TAAAAAGCAGCTGTTTACTCAAGAAGAGTTGGTTCAGGTTAAACGACGCATTGTTAGTAGCATTATGTCAACTGAGTCAG
CAGAACTTGCACAATGGAGAGATAAAGAGCTAGCTGCTAACCCTGAAGATTCATTTATTGAGCGCCGAATTCGTGTTGCT
ATTCGTGCAGCTGATTGGGATGACGTAAGCAACTGGATTGAGTATTTAACACCAACAGCTAAAAACTCATTACGTTGGCA
GTTCTGGACGGCACAGATTGAAAGTAAAGAAGGTGAGCAAGATAAAGCTGATGCTCGTTTAAAAAAATTACTTGGTCAGC
GTAATTTTTATAGTGTTGCTGCGGCAAATATTTTAGGTGAACCTATTCAATATCCAATGAATACCGCCCCTAGAAATATT
AAGCCGATTAAAGATAAATACAGTGTAGAGCTTGCACGTATTAAAGAATTAATTGCGATTGATGAGATCCCTACAGCCAA
AAGTGAGTGGGAATATTTGCTAAATCGAGCATCGAAAACTGAAGTGAAAGAATTAGCAAGTTATGCTGCTCAGCATCGTT
GGCATCACTTTACTGTTCTGGCGACCATCAAAGGAAGAATGTGGGGATATTTGAGCTTACGTTTCCCTATAGCACATCAG
TGGTGGTTTAATCATTACAGTAAGGAACTTGGTTTAGATAAAGTGACGTTAATGTCGTTATCTCGACAAGAAAGTGCATT
GTATGCAGAAGCTCAATCACCGGTAGGTGCTCGTGGTTTGATGCAAATTATGCCAGCAACAGCGAAATATACCGCGAAGA
AGATTGATTACGATAAATACGAAGGTGTTGATAGTTTGAATGATGTTGATGTGAATATTCACATTGGTTCAAATTACCTT
AAGGGTTTATTAGATGATTATGATGGAAACCGTATTTTTGCTCTTGCAGGTTATAATGCCGGCCCTCATCGTGTTAAGCG
TTGGAGAGCGGTTTCTGATGAAAAGCTAGATGCTTATGCTTTTATTGAAGCTATTCCATTCAAGGAAACTAGAGGGTATG
TACAAAACATTTTAATGTTTGAAACTTACTATCGTAGTTTATTGGGTGAAAAAGGTGCTTTTTTATCCGAGAAAGAGCTG
AATTTAAAGTATTGA

Upstream 100 bases:

>100_bases
ATATAAGTAAACTAAGGTAAGAATTCGGTAGATGTATAAGTGAGTCTCAGATCACTACGCCGCAGTCAAAGGTTGGAAAG
TTTAGGTGTTAAAGAAATCA

Downstream 100 bases:

>100_bases
TAAGTTTAAATTCTAATAGCAATTGGTATTAAGCTTAAATAAATATAAAGTAGGGGACTTAATTGTCCCCTTTTTTACAA
TTGTATGAGGATACTATGTC

Product: lytic murein transglycosylase, soluble

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MKYTQYKLGMMTALCIGMNVQVVDASPVDTWRAQYQEAKTLLNEKKYDEYKKVRAELDGYSLAPYLDYRELRMDLSSKTP
DDIRLFKHNHQSLPIGNSLSYQYLVILGLNERWAEFIDYFPNEPSSKNLQCYYYQAKNKLGDKATAWKGAEQLWLTGSSV
TYECDDLFQDWAEAGKLSDDLIIERMLLVYKARNNNLFSYLEKMLKADAAKEKAQHIVTLFNDPDLLESFSKSNKKTEFS
KQLTLISMEREARKNPKKAIEILPDIAKKQLFTQEELVQVKRRIVSSIMSTESAELAQWRDKELAANPEDSFIERRIRVA
IRAADWDDVSNWIEYLTPTAKNSLRWQFWTAQIESKEGEQDKADARLKKLLGQRNFYSVAAANILGEPIQYPMNTAPRNI
KPIKDKYSVELARIKELIAIDEIPTAKSEWEYLLNRASKTEVKELASYAAQHRWHHFTVLATIKGRMWGYLSLRFPIAHQ
WWFNHYSKELGLDKVTLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVDSLNDVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSDEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGAFLSEKEL
NLKY

Sequences:

>Translated_644_residues
MKYTQYKLGMMTALCIGMNVQVVDASPVDTWRAQYQEAKTLLNEKKYDEYKKVRAELDGYSLAPYLDYRELRMDLSSKTP
DDIRLFKHNHQSLPIGNSLSYQYLVILGLNERWAEFIDYFPNEPSSKNLQCYYYQAKNKLGDKATAWKGAEQLWLTGSSV
TYECDDLFQDWAEAGKLSDDLIIERMLLVYKARNNNLFSYLEKMLKADAAKEKAQHIVTLFNDPDLLESFSKSNKKTEFS
KQLTLISMEREARKNPKKAIEILPDIAKKQLFTQEELVQVKRRIVSSIMSTESAELAQWRDKELAANPEDSFIERRIRVA
IRAADWDDVSNWIEYLTPTAKNSLRWQFWTAQIESKEGEQDKADARLKKLLGQRNFYSVAAANILGEPIQYPMNTAPRNI
KPIKDKYSVELARIKELIAIDEIPTAKSEWEYLLNRASKTEVKELASYAAQHRWHHFTVLATIKGRMWGYLSLRFPIAHQ
WWFNHYSKELGLDKVTLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVDSLNDVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSDEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGAFLSEKEL
NLKY
>Mature_644_residues
MKYTQYKLGMMTALCIGMNVQVVDASPVDTWRAQYQEAKTLLNEKKYDEYKKVRAELDGYSLAPYLDYRELRMDLSSKTP
DDIRLFKHNHQSLPIGNSLSYQYLVILGLNERWAEFIDYFPNEPSSKNLQCYYYQAKNKLGDKATAWKGAEQLWLTGSSV
TYECDDLFQDWAEAGKLSDDLIIERMLLVYKARNNNLFSYLEKMLKADAAKEKAQHIVTLFNDPDLLESFSKSNKKTEFS
KQLTLISMEREARKNPKKAIEILPDIAKKQLFTQEELVQVKRRIVSSIMSTESAELAQWRDKELAANPEDSFIERRIRVA
IRAADWDDVSNWIEYLTPTAKNSLRWQFWTAQIESKEGEQDKADARLKKLLGQRNFYSVAAANILGEPIQYPMNTAPRNI
KPIKDKYSVELARIKELIAIDEIPTAKSEWEYLLNRASKTEVKELASYAAQHRWHHFTVLATIKGRMWGYLSLRFPIAHQ
WWFNHYSKELGLDKVTLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVDSLNDVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSDEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGAFLSEKEL
NLKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=636, Percent_Identity=33.6477987421384, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74588; Mature: 74588

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTQYKLGMMTALCIGMNVQVVDASPVDTWRAQYQEAKTLLNEKKYDEYKKVRAELDGY
CCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLAPYLDYRELRMDLSSKTPDDIRLFKHNHQSLPIGNSLSYQYLVILGLNERWAEFIDYF
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHC
PNEPSSKNLQCYYYQAKNKLGDKATAWKGAEQLWLTGSSVTYECDDLFQDWAEAGKLSDD
CCCCCCCCEEEEEEECCCCCCCCHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCHH
LIIERMLLVYKARNNNLFSYLEKMLKADAAKEKAQHIVTLFNDPDLLESFSKSNKKTEFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCHHHH
KQLTLISMEREARKNPKKAIEILPDIAKKQLFTQEELVQVKRRIVSSIMSTESAELAQWR
HHHHHEEEHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHC
DKELAANPEDSFIERRIRVAIRAADWDDVSNWIEYLTPTAKNSLRWQFWTAQIESKEGEQ
CCCCCCCCCHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCC
DKADARLKKLLGQRNFYSVAAANILGEPIQYPMNTAPRNIKPIKDKYSVELARIKELIAI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DEIPTAKSEWEYLLNRASKTEVKELASYAAQHRWHHFTVLATIKGRMWGYLSLRFPIAHQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCHHHH
WWFNHYSKELGLDKVTLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCHHH
EGVDSLNDVDVNIHIGSNYLKGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSDEKLDAYA
CCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHH
FIEAIPFKETRGYVQNILMFETYYRSLLGEKGAFLSEKELNLKY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKYTQYKLGMMTALCIGMNVQVVDASPVDTWRAQYQEAKTLLNEKKYDEYKKVRAELDGY
CCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLAPYLDYRELRMDLSSKTPDDIRLFKHNHQSLPIGNSLSYQYLVILGLNERWAEFIDYF
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHC
PNEPSSKNLQCYYYQAKNKLGDKATAWKGAEQLWLTGSSVTYECDDLFQDWAEAGKLSDD
CCCCCCCCEEEEEEECCCCCCCCHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCHH
LIIERMLLVYKARNNNLFSYLEKMLKADAAKEKAQHIVTLFNDPDLLESFSKSNKKTEFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCHHHH
KQLTLISMEREARKNPKKAIEILPDIAKKQLFTQEELVQVKRRIVSSIMSTESAELAQWR
HHHHHEEEHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHC
DKELAANPEDSFIERRIRVAIRAADWDDVSNWIEYLTPTAKNSLRWQFWTAQIESKEGEQ
CCCCCCCCCHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCC
DKADARLKKLLGQRNFYSVAAANILGEPIQYPMNTAPRNIKPIKDKYSVELARIKELIAI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DEIPTAKSEWEYLLNRASKTEVKELASYAAQHRWHHFTVLATIKGRMWGYLSLRFPIAHQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCHHHH
WWFNHYSKELGLDKVTLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCHHH
EGVDSLNDVDVNIHIGSNYLKGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSDEKLDAYA
CCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHH
FIEAIPFKETRGYVQNILMFETYYRSLLGEKGAFLSEKELNLKY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]